Laboratory of Clinical Analysis

Santo André, Brazil

Laboratory of Clinical Analysis

Santo André, Brazil
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Mateus V.,Polytechnic Institute of Lisbon | Mateus V.,University of Lisbon | Rocha J.,University of Lisbon | Alves P.,University of Coimbra | And 5 more authors.
Basic and Clinical Pharmacology and Toxicology | Year: 2017

Erythropoietin is a potent stimulator of erythroid progenitor cells, which is able to inhibit NF-kB activation, due to its pleiotropic properties, thus promoting an anti-inflammatory effect. As inflammatory bowel disease is a chronic disease with reduced quality of life, and the current pharmacotherapy only induces or maintains the patient in remission, there is a crucial need of new pharmacological approaches. The main objective of this study was to evaluate the effect of erythropoietin in the TNBS-induced colitis model in mice with a normal intestinal flora. Mice with TNBS-induced colitis were treated with a daily dose of erythropoietin at 500 IU/kg bw/day and 1000 IU/Kg bw/day IP during 4 days. As to clinical symptoms/signs, erythropoietin attenuated the decreased body-weight and reduced diarrhoea and oedema of the anus registered in the non-treated mice group in a dose-dependent manner. The anti-inflammatory properties of erythropoietin in the TNBS-induced colitis were confirmed by suppression of pro-inflammatory mediators, such as TNF-α, IL-1β and MPO, as well as a significant increase in the anti-inflammatory cytokine, IL-10, was promoted. These treated mice also presented a reduction in haemoglobin faecal and ALP, suggesting a beneficial effect of erythropoietin in the haemorrhagic focus and destruction of the enterocyte associated with the colon injury induced by TNBS, respectively. The histopathological score was reduced after treatment with erythropoietin, decreasing the severity and extension of the colitis. Furthermore, renal and hepatic biomarkers, as well as haematocrit concentration, remained stabilized after treatment. In conclusion, erythropoietin reduces the inflammatory response associated with TNBS-induced colitis in mice. © 2016 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society)


Bono Battistoni M.F.,National University of Santa | Orcellet V.,National University of Santa | Peralta J.L.,National University of Santa | Marengo R.,National University of Santa | And 7 more authors.
Veterinary Parasitology: Regional Studies and Reports | Year: 2016

An Argentinian Dogo which suffered from anorexia, lymphadenopathy, cachexia and paresis of the hind limbs was diagnosed with trypanosomiasis in Argentina in 2013. In this study, we describe the clinical profile and its evolution as well as the molecular method employed to identify and quantify Trypanosoma evansi. © 2016 Elsevier B.V.


Tofoli G.R.,Universidade São Francisco | Cereda C.M.S.,University of Campinas | Araujo D.R.,Federal University of ABC | Franz-Montan M.,University of Campinas | And 6 more authors.
Journal of Pharmacy and Pharmacology | Year: 2012

Objectives The pharmacokinetics of commercial and liposome-encapsulated mepivacaine (MVC) injected intra-orally in healthy volunteers was studied. Methods In this double blind, randomized cross-over study, 15 volunteers received, at four different sessions, 1.8 ml of the following formulations: 2% MVC with 1: 100 000 epinephrine (MVC 2%EPI), 3% MVC (MVC 3%), 2% and 3% liposome-encapsulated MVC (MVC 2%LUV and MVC 3%LUV). Blood samples were collected pre dose (0 min) and at 15, 30, 45, 60, 90, 120, 180, 240, 300, 360 min after injections. Liquid chromatography-tandem mass spectrometry was used to quantify plasma MVC concentrations. Results Pharmacokinetic analysis showed that the maximum plasma concentration (Cmax) and the areas under the curves (AUC 0-360 and AUC 0-∞) after MVC 2%LUV and MVC 2%EPI injections were smaller (P < 0.05) than the equivalent figures for MVC 3% and MVC 3%LUV. The time to maximum plasma concentration (Tmax) and the half-life of elimination (t1/2beta) obtained after the treatment with MVC 2%LUV, MVC 2%EPI, MVC 3% and MVC 3%LUV presented no statistically significant differences (P > 0.05). Cmax, AUC 0-360 and AUC 0-∞ after injection of the 2% formulations (MVC 2%LUV and MVC 2%EPI) did not exhibit statistically significant differences (P > 0.05). The pharmacokinetics of MVC 2%LUV were comparable to the pharmacokinetics of MVC 2%EPI. Conclusion The liposomal formulation of 2% MVC exhibits similar systemic absorption to the local anesthetic with vasoconstrictor. © 2011 Royal Pharmaceutical Society.


Levis A.,SS Antonio and Biagio Hospital | Stacchini A.,San Giovanni Battista Hospital | Ciriello M.M.,SS Antonio and Biagio Hospital | Geuna M.,Ordine Mauriziano Hospital | And 14 more authors.
European Journal of Haematology | Year: 2011

Objectives:Morphology and cytogenetics are currently used to define prognosis in myelodysplastic syndromes (MDS). However, these parameters have some limits. Flow cytometry has been recently included in the diagnostic panel for MDS, and its prognostic significance is under evaluation. Methods: Marrow aspirates from 424 MDS patients were analyzed by flow cytometry to evaluate the impact of bone marrow cell immunophenotype on overall survival (OS) and leukemia-free survival (LFS). The immature compartment of myeloblasts was analyzed by the quantitative expression of CD34 (<3% vs. ≥3%), CD117, and CD11b -/CD66b - (<5% vs. ≥5%); myeloid maturation was analyzed by the expression of CD11b +/CD66b ++ (<15% vs. ≥15%) and CD11b +/CD66b + (<25% vs. ≥25%). Results:  In univariate analysis, the expression of immaturity markers (CD34 +, CD117 +, and CD11b -/CD66b -) was associated with shorter LFS and OS (P<0.0001); higher expression of differentiation markers (CD11b +/CD66b ++ and CD11b +/CD66b +) was associated with longer LFS (P<0.0001 and P=0.0002, respectively) and OS (P<0.0001). In multivariate analysis, expression of CD34 + (P=0.007), CD117 + (P=0.013), and CD11b +/CD66b ++ (P=0.023) retained independent prognostic value for OS, while only the expression of CD34 + was a prognostic factor for LFS (P=0.0003). Two different risk groups were defined according to the presence of 0-1 or ≥2 of these factors with significant different LFS and OS (P<0.0001). This score showed prognostic value in predicting survival even in subanalysis according to IPSS and WHO subgroups. Conclusions: Flow cytometric analysis in MDS may provide meaningful prognostic information. Blast percentage expressed as CD117 + or CD34 + cells and the quantitative assessment of myeloid maturation showed prognostic value for survival. © 2011 John Wiley & Sons A/S.


Rampelotto R.F.,Federal University of Santa Maria | Bertazzo T.,Federal University of Santa Maria | Weis G.L.,Hospital Universitario Of Santa Maria Husm | de Jesus P.R.O.,Hospital Universitario Of Santa Maria Husm | And 5 more authors.
Scientia Medica | Year: 2017

AIMS: This article reports a case of pyonephrosis caused by Salmonella sp. in a patient with polycystic kidney disease undergoing hemodialysis treatment. CASE DESCRIPTION: An elderly male patient previously diagnosed with polycystic kidney disease undergoing standard hemodyalitic treatment presented uronephrosis, evolving to pyonephrosis caused by Salmonella sp., and was successfully treated with ciprofloxacin. CONCLUSIONS: The polycystic kidney disease may have contributed to the bacteria's attachment to the kidney, due to increase of permeability of the intestinal mucosa, easier bacterial translocation to bloodstream and its subsequent accommodation in the infected organ.


Miranda D.O.,Federal University of Uberlandia | Silva D.A.O.,Federal University of Uberlandia | Fernandes J.F.C.,Federal University of Uberlandia | Queirs M.G.J.,Federal University of Uberlandia | And 7 more authors.
Clinical and Developmental Immunology | Year: 2011

Allergic rhinitis (AR) is a public health problem with high prevalence worldwide. We evaluated levels of specific IgE, IgA, and IgG4 antibodies to the Dermatophagoides pteronyssinus (Dpt) house dust mite and to its major allergens (Der p1 and Der p2) in serum and saliva samples from allergic and nonallergic children. A total of 86 children were analyzed, from which 72 had AR and 14 were nonallergic healthy children. Serum IgE and serum/salivary IgG4 levels to Dpt, Der p1, and Der p2 were higher in allergic children whereas serum/salivary IgA levels to all allergens were higher in nonallergic children. IgE levels positively correlated with IgG4 and IgA to all allergens in allergic children, while IgA levels negatively correlated with IgG4 to Dpt and Der p1 in nonallergic children. In conclusion, mite-specific IgA antibodies predominate in the serum and saliva of nonallergic children whereas mite-specific IgE and IgG4 are prevalent in allergic children. The presence of specific IgA appears to have a key role for the healthy immune response to mucosal allergens. Also, specific IgA measurements in serum and/or saliva may be useful for monitoring activation of tolerance-inducing mechanisms during allergen specific immunotherapeutic procedures, especially sublingual immunotherapy. Copyright © 2011 Diego O. Miranda et al.


Sebastia C.,Laboratory of Clinical Analysis | Soriano J.M.,University of Valencia | Iranzo M.,University of Valencia | Rico H.,University of Valencia
Journal of Food Processing and Preservation | Year: 2010

The effect of three different periods of storage life (0, 15 and 30 days) on the microbiological quality of several foods elaborated with the sous vide cook-chill procedure in a restaurant was studied. These foods are grouped in three categories: meat (breast chicken, foie gras, pork loin and entrecote of veal), fish (codfish, gilt head sea bream, hake and salmon) and vegetable (broccoli, courgette, potato and carrot). Total plate counts in the products studied were in the range of <1.00-3.74 log10 cfu/g. Coagulase negative staphylococci were isolated in the three categories, Enterococcus spp. were isolated from foie gras and Pantoea agglomerans from salmon and carrot. Staphylococcus aureus, Escherichia coli, Salmonella spp., Listeria monocytogenes and Clostridium perfringens were not detected in any of the samples studied. As a consequence of our study, the restaurant has developed and implemented a traceability system to achieve food safety. © 2010 Wiley Periodicals, Inc.


Bello S.R.,Anhembi Morumbi University | Batestusso R.S.,Anhembi Morumbi University | De Oliveira C.G.B.,Laboratory of Clinical Analysis | Fonseca F.L.A.,Laboratory of Clinical Analysis | And 2 more authors.
Journal of Obstetrics and Gynaecology Research | Year: 2010

Aim: The purpose of this research was to assess the effects of the flower essences She Oak and Bush Fuchsia on behavioral anxiety in ovariectomized (OVX) rats. Methods: For four weeks, OVX rats received the flower essences She Oak, Bush Fuchsia or a combination of the two. After flower therapy, the animals were subjected to an elevated plus maze (EPM) behavioral anxiety-test. Cortisol blood level was also evaluated. Results: OVX rats treated with the flower essence She Oak became less anxious and had more entries in the EPM open arms. On the other hand, OVX rats treated with the Bush Fuchsia essence spent more time in the EPM closed arms. This finding is similar to those obtained with controls. In addition, OVX rats that received She Oak and Bush Fuchsia in combination presented the same results as those receiving the Bush Fuchsia alone. Conclusions: Our results suggest that the flower essence She Oak could have an anxiolytic effect in OVX rats, but that the combination therapy of the She Oak and Bush Fuchsia could avoid the effects of the She Oak. © 2010 Japan Society of Obstetrics and Gynecology.


Braggion G.F.,São Judas Tadeu University | Ornelas E.,São Judas Tadeu University | Cury J.C.S.,São Judas Tadeu University | Lima N.E.A.,São Judas Tadeu University | And 4 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2016

Introduction. Sarcopenia is a process characterized by reduction in protein mass and muscle strength with increasing age, especially in the postmenopausal period, resulting in functional limitations and with great impact on the physical autonomy of the elderly. Objective. To evaluate the effects of diets with vegetable proteins (VP) or animal proteins (AP) associated with resistance training (RT) on the structural and biochemical parameters of the medial gastrocnemius muscle in Wistar rats with sarcopenia. Methods. An experimental model with ovariectomized rats was used to induce sarcopenia and resistance training. The histochemical technique was used for the typing of muscle fibers, the cross-sectional area of myocytes, and volume densities of myocytes and interstitium; the technique of Picrosirius stain was used to highlight the collagen fibers. Results. The VP diet was not able to minimize the effects of sarcopenia in the medial gastrocnemius of sedentary animals and when associated with RT, it promoted maintenance of the CSA, attenuating the atrophy of type IIB fibers in the medial gastrocnemius. The AP diet in sedentary animals protected the type I fibers. When combined with RT, the AP promoted muscle remodeling, with reduction in volume density of type I and IIA fibers, and increase of IIB fibers, together with an increase in collagen volume density. Conclusion. The data suggest a tendency to better results of hypertrophy in animal groups that consumed the AP diet, even the sedentary animals, although more evident in those trained. © 2016 Glaucia Figueiredo Braggion et al.

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