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Souza-Santos L.P.,Federal University of Pernambuco | De Lima L.C.M.,Federal University of Pernambuco | Navarro D.M.A.F.,Laboratory of Chemical Ecology
Crustaceana | Year: 2013

The present study assessed the efficiency of two transesterification methods for the identification of fatty acids in the copepod Tisbe biminiensis Volkmann-Rocco, 1973. In the first method, the samples were esterified by basic catalysis with KOH in methanol, and in the second method they were esterified by acid catalysis with BF3. Fatty acids were identified by comparison of retention times and co-injection with standard Mix C4-C24, using gas chromatography and gas chromatography-mass spectrometry. Twenty-nine fatty acids were detected in samples transesterified by basic catalysis or acid catalysis. The predominant fatty acids were C18 (19.18%), C16 (13.02%), and C12 (11.39%) for samples transesterified by acid catalysis; and C13 (25.24%), C21 (11.32%), and C18 (10.65%) for samples transesterified by basic catalysis. The results show that fatty acid analysis for copepods may be affected by the transesterification method. Sample transesterification by acid catalysis was efficient to identify monosaturated and polyunsaturated fatty acids, whereas transesterification by basic catalysis was efficient to identify saturated fatty acids. Essential fatty acids were present in the copepod, which suggests that T. biminiensis is a useful food to be used in larviculture. © 2013 Koninklijke Brill NV, Leiden.

Urzua A.,Laboratory of Chemical Ecology | Espinoza J.,Laboratory of Chemical Ecology | Olguin A.,Laboratory of Chemical Ecology | Santander R.,Laboratory of Chemical Ecology
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas | Year: 2013

Three phenolic aristolactams, aristolactam AII (3), velutinam (4) and piperolactam A (5), were identified from the leaves and stems of Aristolochia chilensis Bridges ex Lindl. The structures of these compounds were elucidated using a combination of HPLC-DAD, GC-MS and NMR experiments. © 2013 Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas.

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