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Gdoura N.,University of Sfax | Abdelmouleh A.,University of Sfax | Chaabouni K.,Laboratory of Biochemistry and Biophysics | Ayadi F.M.,Laboratory of Biochemistry and Biophysics | And 3 more authors.
Environmental Chemistry Letters | Year: 2011

Fish, a widely claimed healthy food for humans, could also pose problems to health due to (1) accumulation of pollutants, especially heavy metals, (2) presence of marine toxins such as tetrodotoxin, ciguatoxin or okadaic acid and (3) metabolic production of large amount of uric acid which may crystallize in kidney or in articulations. As tuna fish represents a large part of the traditional food in some countries, the possible impact of its consumption on public health was investigated, with special attention to biochemical and histological alterations related to the genital function. For that purpose, white or red muscle from tuna fish was added for 60 days to the diet of male rats, and attention was paid to biochemical and histological alterations related to the genital function. Feeding rats with white muscle, and much more markedly with red muscle known to be more metabolically active, resulted in (1) an elevated uric acid level in blood, (2) an increase in lead level in testis, (3) an atrophy of the genital tract, including testes, epididymis, prostate and seminal vesicles, (4) a lowering of plasma testosterone level, (5) a decrease in spermatozoids number and motility, (6) an oxidative stress in testes including an increase in lipids peroxidation level and enhanced superoxide-dismutase, catalase and glutathione-peroxidase activities. We conclude that consumption of tuna fish meat, especially the dark one, should be reduced. © 2010 Springer-Verlag.

Abdulazeez M.A.,Ahmadu Bello University | Ibrahim S.,Ahmadu Bello University | Ameh D.A.,Ahmadu Bello University | Ayo J.O.,Ahmadu Bello University | Carvalho L.J.C.,Laboratory of Biochemistry and Biophysics
Romanian Biotechnological Letters | Year: 2013

The anticancer activity of leaf extracts of Peristrophe bicalyculata was evaluated. Results showed that the methanolic ethylacetate fraction with an IC50 of 15.60 ± 0.52 μg/ml was potentially very toxic against human mouth epidermal carcinoma (KB) cells. After partial purification of the methanolic ethylacetate fraction, seven fractions were obtained; five of which were inactive (IC50 values above 500 μg/ml) and one moderately toxic (IC50 = 235 ± 33.05 μg/ml), hence not considered. The IC50 value of the most cytotoxic fraction (fraction 5) (3.50 ± 0.21 μg/ml) was not different from that of cisplastin (3.32 ± 0.09 μg/ml), but was significantly (P<0.05) higher than the value obtained for doxorubicin (0.43 ± 0.02 μg /ml) and vincristine (0.05 ± 0.01μg/ml). The partially-purified fraction induced apoptosis in KB cells after 24 and 48 hours in a dose-dependent manner. The percentage of apoptotic cells in cultures treated with this fraction was significantly (P<0.05) higher than that in control cultures, but lower than cultures treated with cisplastin. The GC-MS analysis of the fraction indicated an abundance of andrographolide 2(3H)-furanone and aromadendrene oxide, both of which possess anticancer properties. The results provide scientific evidence supporting the anticancer activity of the methanolic ethylacetate fraction of the plant. In conclusion, the methanolic ethylacetate fraction of Peristrophe bicalyculata is a potential source of chemotherapeutic agent. © 2013 University of Bucharest.

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