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Storelli N.,University of Geneva | Storelli N.,Laboratory of Applied Microbiology | Saad M.M.,University of Geneva | Frigaard N.-U.,Copenhagen University | And 4 more authors.
EuPA Open Proteomics | Year: 2014

Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea. © 2013 The Authors.


Trivellone V.,Station de Recherche Agroscope Changins Wadenswil ACW | Trivellone V.,Swiss Federal Institute of forest | Trivellone V.,University of Neuchatel | Filippin L.,Crea Vit Centro Of Ricerca Per La Viticoltura | And 2 more authors.
European Journal of Plant Pathology | Year: 2016

The most important Grapevine Yellows (GY) phytoplasma diseases in Europe are Flavescence dorée (FD) and Bois noir (BN); they are spread in vineyard by two proven vectors, Scaphoideus titanus Ball and Hyalesthes obsoletus Signoret, respectively. Other potential vectors of GY have been identified, which are thought to play a secondary role. The GY control strategies are not always effective and an in-depth study on the ecological cycle of the pathogens at regional scale would be of paramount importance. This study was carried out in 48 representative sites of wine-growing area South of Swiss Alps, with the aim to identify known and potential vectors and to characterize the FD and BN phytoplasmas isolates. Out of 167 Auchenorrhyncha species recorded, 27 were known or potential vectors of phytoplasmas and five of those tested positive for phytoplasmas. S. titanus was infected by 16SrV-D subgroup phytoplasma and no clear relationship between its population density and disease outbreaks was observed. Orientus ishidae harboured 16SrV-C and 16SrV-D subgroups suggesting its potential role in spreading 16SrV-C phytoplasma isolates from arboreal plants to grapevine and FD-D from grapevine to grapevine. H. obsoletus was infected by BN phytoplasmas, tuf-types a and b, however it was collected with relatively low abundance. Reptalus panzeri and R. cuspidatus tested positive to tuf-type b, but only R. cuspidatus was common and abundant in the investigated vineyards. To define the range of alternative vectors using a detailed approach on regional scale provides background information to get a more clear vision on the spreading of phytoplasmas in the vineyards. © 2016 Koninklijke Nederlandse Planteziektenkundige Vereniging


Mouloud G.,Laboratory of Applied Microbiology | Mouloud G.,Laboratory of Plant Biotechnology | Daoud H.,Laboratory of Applied Microbiology | Bassem J.,Instituto Valenciano Of Investigaciones Agrarias | And 2 more authors.
Applied Biochemistry and Biotechnology | Year: 2013

A bacteriocin-producing strain (9,000 AU/ml) was isolated from the rhizosphere of Algerian healthy plants Ononis angustissima Lam. and identified as Bacillus clausii strain GM17. The bacteriocin, called Bac-GM17, was purified fromthe culture supernatant after heat treatment, ammonium sulfate precipitation, Sephadex G-50 chromatography andMono Q fast-performance liquid chromatography (FPLC). Based on matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis, the purified Bac-GM17 is amonomer protein with a molecular mass of 5,158.11 Da. The N-terminal sequencing allowed for the straightforward identification of its first 20 residues, which were of pure bacteriocin. It also revealed that this bacteriocin contained a unique sequence, namely DWTCSKWSCLVCDDCSVELT, which suggests the identification of a novel compound. Bac-GM17 was extremely heat stable (20 min at 120 °C) and was stable within the pH range (3-9). It was found to be resistant to the proteolytic action of trypsin, pepsin, papain, pronase E, and proteinase K. It was also noted to display a bactericidal mode of action against Agrobacterium tumefaciens C58 and a fungistatic mode of action against Candida tropicalis R2 CIP203. © Springer Science+Business Media New York 2013.


Toho K.,Laboratory of Applied Microbiology | Abe N.,Laboratory of Applied Microbiology | Yamada T.,Tohoku University | Ito Y.,Laboratory of Applied Microbiology | And 3 more authors.
Letters in Organic Chemistry | Year: 2011

(γ-L-Glutamyl)putrescine and N1- and N8- (γ-L-glutamyl)spermidines, possible regioisomeric products of the polyamine glutamyl transferase of bacteria such as Escherichia coli and Pseudomonas aeruginosa, were synthesized from N-Boc-protected putrescine and 1,3-propanediamine. © 2011 Bentham Science Publishers Ltd.


Thang V.H.,Laboratory of Applied Microbiology | Thang V.H.,Hanoi University of Science and Technology | Kanda K.,Laboratory of Applied Microbiology | Kobayashi G.,Laboratory of Applied Microbiology
Applied Biochemistry and Biotechnology | Year: 2010

In this work, acetone-butanol-ethanol (ABE) fermentation characteristics of cassava starch and cassava chips when using Clostridium saccharoperbutylacetonicum N1- 4 was presented. The obtained results in batch mode using a 1-L fermenter showed that C. saccharoperbutylacetonicum N1-4 was a hyperamylolytic strain and capable of producing solvents efficiently from cassava starch and cassava chips, which was comparable to when glucose was used. Batch fermentation of cassava starch and cassava chips resulted in 21.0 and 19.4 g/L of total solvent as compared with 24.2 g/L of total solvent when using glucose. Solvent productivity in fermentation of cassava starch was from 42% to 63% higher than that obtained in fermentation using corn and sago starches in the same condition. In fermentation of cassava starch and cassava chips, maximum butanol concentration was 16.9 and 15.5 g/L, respectively. Solvent yield and butanol yield (based on potential glucose) was 0.33 and 0.41, respectively, for fermentation of cassava starch and 0.30 and 0.38, respectively for fermentation using cassava chips. © Humana Press 2009.

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