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Yaoundé, Cameroon

Kazeminia S.M.,Payame Noor University | Kazeminia S.M.,Laboratory of Animal Physiology | Kalaee S.E.V.,Payame Noor University | Nasri S.,Payame Noor University
Journal of Mazandaran University of Medical Sciences | Year: 2014

Background and purpose: Imbalances in sex steroid hormone levels are strongly associated with diabetes and this may negatively impact upon sexual function. The aim of this study was to investigate the protective role of alcoholic extract of palm pollen (L.Phoenix dactylifera) on testosterone, LH and FSH levels in adult male diabetic rats. Material and Methods: In this experimental study, thirty adult male Wistar rats (250±23 gr) were selected and randomly divided into three groups (n=10): (i) control; (ii) diabetic and (iii) treatment. Diabetes was induced by a single injection of streptozotocin (55 mg/kg, i.p). The treatment group received 0.2 mg/kg alcoholic extract of pollen of Phoenix dactylifera daily (gavages) for four weeks. At the end of experiments, the rats were anesthetized by injection of pentobarbital sodium (60 mg/kg i.p.) and sacrificed. Blood samples were taken from the left ventricle and testicular tissues were weighed and prepared for histological examination upon removal. Blood serum was separated and immediately assayed for LH, FSH and testosterone by ELISA method. The comparisons were carried out using one-way analysis of variance (ANOVA) followed by post-hoc Tukey test (package of SPSS, version 19) Results: The findings revealed that the mean of testosterone level in the diabetic group declined significantly (P<0.05) and testicular and epididymis weight in diabetic group significantly reduced in comparison with the control group (P<0.05). In addition, in the diabetic group, disintegration of tubular cells, vacuolization of spermatogonia cells were seen in most of seminiferous tubules. Also, spermatozoa were rarely seen in seminiferous tubules in comparison with control group.The LH and FSH level did not show any significant difference between groups. However, in treatment group, the testosterone level significantly increased in comparison with the diabetic group (P<0.05). Histopathological findings of the treatment group were similar to control group. Conclusion: According to the results, it is suggested that the extract of pollen of Phoenix dactylifera may improve and protect testis structure diabetic rats and may have a regulatory effect on diabetes-induced change of the level of testosterone hormone in diabetic male rats. Source

Habara M.,Nippon Veterinary and Life Science University | Tamanuki M.,Nippon Veterinary and Life Science University | Ishikawa S.,Nippon Veterinary and Life Science University | Takemitsu H.,Nippon Veterinary and Life Science University | And 6 more authors.
Asian Journal of Animal and Veterinary Advances | Year: 2015

G-protein-coupled receptors (GPRs) 40 and 120 are members of the Free Fatty Acid (FFA) receptor group and are termed FFAR1 and FFAR4, respectively. The aim of this study was to clone cat GPR40 and GPR120 cDNAs in several tissues. There was high sequence homology to other mammalian GPR40 and GPR120, with encoding 320 and 361 amino acid residues, respectively. Cat GPR40 encoded extra 21 amino acid residues in the C-terminal cytoplasmic region. Quantitative RT-PCR revealed expression of GPR40 mRNA in the duodenum, liver and pancreas. The GPR120 mRNA was expressed in adipose tissues, cerebral cortex and colon. In conclusion, GPR40 and GPR120 were well conserved and were expressed in cat tissues with different distribution patterns. © 2015 Academic Journals Inc. Source

Adachi A.A.,Kinki University | Fujioka A.,Kinki University | Nagano M.,Kinki University | Masumoto K.-H.,Kinki University | And 4 more authors.
Zoological Science | Year: 2013

The mammalian circadian oscillator is composed of interacting positive and negative transcription events. The clock proteins PER1 and PER2 play essential roles in a negative limb of the feedback loop that generates the circadian rhythm in mammals. In addition, the proteins CLOCK and BMAL1 (also known as ARNTL) form a heterodimer that drives the Per genes via the E-box consensus sequences within their promoter regions. In the present study, we demonstrate that Id2 is involved in stabilization of the amplitudes of the circadian oscillations by suppressing transcriptional activation of clock genes Clock and Bmal1. Id2 shows dynamic oscillation in the SCN, with a peak in the late subjective night. Under constant dark conditions (DD), Id2-/- mice showed no apparent difference in locomotor activity, however, under constant light conditions (LL), Id2-/- mice exhibit aberrant locomotor activity, with lower circadian oscillation amplitudes, although the free running periods in Id2-/- mice show no differences from those in either wild type or heterozygous mice. Id2-/- animals also exhibit upregulation of Per1 in constant light, during both the subjective night and day. In wild type mice, Id2 is upregulated by constant light exposure during the subjective night. We propose that Id2 expression in the SCN contributes to maintenance of dynamic circadian oscillations. © 2013 Zoological Society of Japan. Source

Anestis A.,Laboratory of Animal Physiology | Portner H.O.,Alfred Wegener Institute for Polar and Marine Research | Karagiannis D.,Aristotle University of Thessaloniki | Angelidis P.,Aristotle University of Thessaloniki | And 2 more authors.
Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology | Year: 2010

In the context of climate change the present work aimed to illustrate whether the energetic and metabolic pattern of mussels Mytilus galloprovincialis will be affected by increase in the temperature of seawater. Moreover we examined whether an outbreak of Marteilia sp. infestation as a result of increase in sea water temperature will impair the energetic balance of mussels. M. galloprovincialis was acclimated at 18 °C, 24 °C, 26 °C and 28 °C for 30 days and the energetic pattern of its tissues was estimated by determining the factor Scope for Growth (SFG), while the metabolic pattern of mussels was estimated by determining the activities of pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK). The decrease in PK activity and the decrease in the ratio PK/PEPCK indicated an activation of anaerobic component of metabolism during acclimation of mussels at temperature 24 °C. Αt temperatures higher than 24 °C the values of SFG turned negative probably associated with a significant reduction in clearance rate. Compared to the non infected mussels, the SFG values of infected mussels were significantly lower (P < 0.05). These differences were attributed to the higher filtration rate and the lower absorption efficiency detected in the infected mussels. Also the degree of SFG reduction is dependent on the intensity levels of infection by Marteilia sp. © 2009 Elsevier Inc. All rights reserved. Source

Kamgang R.,Institute of Medical Research and Medicinal Plants Studies IMPM | Kamgang R.,Laboratory of Animal Physiology | Foyet A.,Laboratory of Animal Physiology | Essame J.-L.,Institute of Medical Research and Medicinal Plants Studies IMPM | And 2 more authors.
Indian Journal of Pharmacology | Year: 2012

Objectives: To investigate the effect of Kalanchoe crenata methanolic fraction (MEKC) on proteinuria, glucosuria, and some other biochemical parameters in adriamycin-induced renal impairment in rats. Materials and Methods: Ether anesthetized rats received three intravenous injections (days 0, 14, and 28) of 2 mg/kg body weight of adriamycin. Repeated doses of the extract (0, 50, and 68 mg/kg b.w.) and losartan (10 mg/kg b.w.) were administered orally once daily, for 6 weeks, to these rats. Kidney functions were assessed through biochemical parameters. Results: MEKC decreased proteinuria and also the urinary excretion of creatinine, glucose, and urea significantly in diseased rats. A decrease in serum levels of creatinine, urea, potassium, alkaline phosphatase, conjugate bilirubin, and alanine transaminase level was also recorded in nephropathic rats, but plasma levels of uric acid and glucose remained unchanged. Moreover, the plant extract markedly (P < 0.05) increased plasma sodium and decreased (P < 0.01) the urinary sodium and potassium levels. Conclusions: The results indicated that the treatment with the methanolic fraction of K. crenata may improve proteinuria and all other symptoms due to adriamycin-induced nephropathy and, more than losartan, could ameliorate kidney and liver functions. K. crenata could be a potential source of new oral antinephropathic drug. Source

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