Villeurbanne, France
Villeurbanne, France

Time filter

Source Type

Zinoubi K.,University of Monastir | Braham Y.,University of Monastir | Barhoumi H.,University of Monastir | Benounis M.,University of Khenchela | Jaffrezic N.,Laboratory of Analytical science
Sensor Letters | Year: 2016

In this work, an electrochemical sensor for the detection of trace amount of heavy metal ions was developed. The sensing proprieties of the (Au-NPs/cysteine composite matrix towards Cu2+, Pb2+ and Cd2+ ions was investigated using differential pulse anodic stripping voltammetry (DPASV). As a result, the modified gold electrode demonstrates a good stability with high responses of 82.5 μA/pPb, 44.7 μA/pCu and 11.92 μA/pCd for different tested metal ions. Consequently, a wide linear response range from 10-8 to 10-5 mol/L and a lower detection limit about 5.10-8 mol/L were obtained. The electrochemical developed sensor was applied for the determination of Cu(II), Pb(II) and Cd(II) concentrations in real environmental water samples. Copyright © 2016 American Scientific Publishers All rights reserved.


Bekir K.,University of Monastir | Bousimma F.,University of Monastir | Barhoumi H.,University of Monastir | Fedhila K.,University of Monastir | And 6 more authors.
Environmental Science and Pollution Research | Year: 2015

In this report, we describe a new immunosensor designed for the detection and the quantification of Pseudomonas aeruginosa bacteria in water. The developed biosensing system was based on the immobilization of purified polyclonal anti P. aeruginosa antibodies on electropolymerized poly(pyrrole-3-carboxylic acid)/glassy carbon electrode. The building of the immunosensor step by step was evaluated by electrochemical measurements such as cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical signature of the immunosensor was established by the change of the charge transfer resistance when the bacteria suspended in solution became attached to the immobilized antibodies. As a result, stable and high sensitive impedimetric immunosensor was obtained with a sensitivity of 0.19 kΩ/decade defined in the linear range from 101 to 107 CFU/mL of cellular concentrations. A low detection limit was obtained for the P. aeruginosa bacteria and a high selectivity when other bacteria were occasioned as well as Escherichia coli. The developed immunosensor was applied in detecting pathogenic P. aeruginosa in well-water. © 2015, Springer-Verlag Berlin Heidelberg.


Ben Ali Hassine C.,Laboratory of Interfaces and Advanced Materials LIMA | Barhoumi H.,Laboratory of Interfaces and Advanced Materials LIMA | Maaref A.,Laboratory of Interfaces and Advanced Materials LIMA | Jaffrezic Renault N.,Laboratory of Analytical science
Sensor Letters | Year: 2016

In this study we report the development of copper ion sensor based on the electrochemical modification of a glassy carbon electrode by aurintricarboxylic acid ammonium salt (Aluminon). The deposited Aluminon layer was characterized by cyclic voltammetry and electrochemical impedance spectroscopy methods. The sensitivity of the developed ion sensor towards some metallic ions was evaluated by square-wave voltammetry. Among some tested metal ions the modified GC electrode shows a high and stable response towards the copper ions with a sensitivity of 23.8 μAM-1 and detection limit around 3.16 10-7 M. In addition, UV-Visible spectrophotometry technique was carried out to confirm the best affinity between ATA and Cu2+ ions. An association constant of the hostguest complex of 20.104 L mol-1 was obtained. As a result, a good selective copper ions sensor was obtained with low detection limit. © 2016 American Scientific Publishers.

Loading Laboratory of Analytical science collaborators
Loading Laboratory of Analytical science collaborators