Laboratory Limbach

Heidelberg, Germany

Laboratory Limbach

Heidelberg, Germany
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Belavy D.L.,Charité - Medical University of Berlin | Seibel M.J.,University of Sydney | Seibel M.J.,Concord Hospital Medical Center | Roth H.J.,Laboratory Limbach | And 4 more authors.
Journal of Endocrinological Investigation | Year: 2012

Background and aim: There is limited data on the effects of inactivity (prolonged bed-rest) on parameters of endocrine and metabolic function; we therefore aimed to examine changes in these systems during and after prolonged (56-day) bed-rest in male adults. Subjects and methods: Twenty healthy male subjects underwent 8 weeks of strict bed-rest and 12 months of follow-up as part of the Berlin Bed Rest Study. Subjects were randomized to an inactive group or a group that performed resistive vibration exercise (RVE) during bed-rest. All outcome parameters were measured before, during and after bed-rest. These included body composition (by whole body dual X-ray absorptiometry), SHBG, testosterone (T), estradiol (E 2), PRL, cortisol (Q, TSH and free T 3 (FT 3). Results: Serum SHBG levels decreased in inactive subjects but remained unchanged in the RVE group (p<0.001). Serum T concentrations increased during the first 3 weeks of bed-rest in both groups (p<0.0001), while E 2 levels sharply rose with re-mobilization (p<0.0001 ). Serum PRL decreased in the control group but increased in the RVE group (p=0.021). C levels did not change over time (p≥0.10). TSH increased whilst FT 3 decreased during bed-rest (p all ≤0.0013). Conclusions: Prolonged bed-rest has significant effects on parameters of endocrine and metabolic function, some of which are related to, or counteracted by physical activity. ©2012, Editrice Kurtis.


Scharnholz B.,University of Heidelberg | Gilles M.,University of Heidelberg | Marzina A.,University of Mannheim | Kommer M.,University of Mannheim | And 9 more authors.
Psychoneuroendocrinology | Year: 2014

This study compared features of the metabolic syndrome between healthy controls and depressed patients without activation of the hypothalamus-pituitary-adrenal (HPA) system. After exclusion of non-suppressors to 1. mg dexamethasone, we included 20 depressed inpatients and 34 healthy controls in the analyses. We assessed HPA system activity (diurnal saliva cortisol profile, cortisol excretion), normetanephrine excretion as well as fasting glucose, lipid profile and blood pressure. With regard to body composition, we measured waist circumference as well as visceral fat and adrenal volume by magnetic resonance (MR) imaging. Five depressed patients (25%) and five healthy controls (15%) fulfilled the criteria of the metabolic syndrome according NCEP-ATP-III. Depression was significantly related with fasting glucose and negatively associated with mean blood pressure (BP) and, by trend, with low HDL-cholesterol. We conclude that depressed patients may have modest metabolic disturbances even in the complete absence of activation of stress-responsive systems. Hence some metabolic disturbances in depressed patients may not be explicable by HPA activation. Additional factors are required to mediate the link between affective and metabolic disorders. © 2013 Elsevier Ltd.


Chase D.J.,Johnson and Johnson GmbH | Schenkel B.P.,Laboratory Limbach | Fahr A.-M.,Laboratory Limbach | Eigner U.,U.S. Department of Agriculture
Journal of Clinical Microbiology | Year: 2010

This study compared the safety of a new tampon with a four-winged apertured film cover over its nonwoven cover to improve leakage performance with that of a commercial tampon with a nonwoven cover only. Healthy women (evaluable, n = 69) were randomized to crossover between test and reference tampons in two consecutive menstrual cycles. Qualitative and quantitative analyses of vaginal cultures were conducted pre-, mid-, and postmenstrually for a broad panel of microorganisms, and colposcopy was performed. Similar to previous studies, prevalence and mean colony counts of the majority of microorganisms generally increased midmenstrually and returned or began to return postmenstrually. In contrast to most previous studies, Lactobacillus species remained at similar levels throughout the cycles with both tampons. Neither tampon was associated with clinically significant microbiological changes or abnormalities or with vaginal/cervical epithelial integrity changes on colposcopy. Microbiological and colposcopic evaluations indicate that the apertured film-covered tampon is safe. Copyright © 2010, American Society for Microbiology. All Rights Reserved.


Ulrich E.,Laboratory Limbach | Veldenzer A.,Laboratory Limbach | Fahr A.-M.,Laboratory Limbach | Holfelder M.,Laboratory Limbach
Clinical Laboratory | Year: 2012

We evaluated the performance of a PCR-based dipstick assay used in our routine laboratory for the direct detection of Methicillin resistant Staphylococcus aureus (MRSA). 2941 clinical swab specimens were evaluated. Sensitivity and specificity were 94.1% and 98.3%, respectively.The PCR assay combines low instrumentation costs and minor hands-on-time with reliable results for MRSA identification.

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