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Busch F.,Ludwig Maximilians University of Munich | Mobasheri A.,University of Nottingham | Shayan P.,Investigating Institute of Molecular Biological System Transfer | Lueders C.,Laboratory for Tissue Engineering | And 2 more authors.
Journal of Biological Chemistry | Year: 2012

Background: Resveratrol has been proposed to have beneficial health effects due to its anti-inflammatory properties. Results: Resveratrol suppressed IL-1β-induced activation of NF-κB and PI3K in a dose- and time-dependent manner. Conclusion: Anti-inflammatory effects of resveratrol may be mediated at least in part through inhibition/deacetylation of PI3K and NF-κB. Significance: Activated Sirt-1 plays an essential role in anti-inflammatory effects of resveratrol. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

Reichardt A.,Laboratory for Tissue Engineering | Arshi A.,RWTH Aachen | Schuster P.,RWTH Aachen | Polchow B.,Laboratory for Tissue Engineering | And 5 more authors.
Journal of Biomaterials and Tissue Engineering | Year: 2012

Polymers composed of polyglycolide (PGA) or polylactide (PLA) have been studied in multiple research projects for orthopedic applications, cardiovascular tissue engineering (TE) and drug delivery systems. In a recent TE study we generated and analyzed custom-made nonwovens composed of PGA, PLA, a composite structure of PLA/PGA, and the copolymer PLG regarding the behavior of cells when seeded onto these structures. The fabrication of the individual nonwovens was optimized for this study. PGA nonwovens revealed notable cell compatibility, with large amounts of extracellular matrix proteins. Unfortunately, rapid shrinking induced by labored hydrolytic degradation and failing resistibility to cells was detected. Incorporation of additional, long-living material, e.g., PLA was essential for the fabrication of an applicable TE substitute. The best results, combining cell compatibility and longevity of the cell-matrix construct, were achieved using the PLA/PGA composite. The durability of PLA/PGA composite structures makes them promising for use in such regions of increased load, as the heart and particularly for the tissue engineering of cardiovascular structures such as heart valves. © 2012 American Scientific Publishers. All rights reserved.

Lueders C.,Laboratory for Tissue Engineering | Jastram B.,TU Berlin | Hetzer R.,Laboratory for Tissue Engineering | Schwandt H.,TU Berlin
European Journal of Cardio-thoracic Surgery | Year: 2014

Three-dimensional (3D) printing technologies have reached a level of quality that justifies considering rapid manufacturing for medical applications. Herein, we introduce a new approach using 3D printing to simplify and improve the fabrication of human heart valve scaffolds by tissue engineering (TE). Custom-made human heart valve scaffolds are to be fabricated on a selective laser-sintering 3D printer for subsequent seeding with vascular cells from human umbilical cords. The scaffolds will be produced from resorbable polymers that must feature a number of specific properties: the structure, i.e. particle granularity and shape, and thermic properties must be feasible for the printing process. They must be suitable for the cell-seeding process and at the same time should be resorbable. They must be applicable for implementation in the human body and flexible enough to support the full functionality of the valve. The research focuses mainly on the search for a suitable scaffold material that allows the implementation of both the printing process to produce the scaffolds and the cell-seeding process, while meeting all of the above requirements. Computer tomographic data from patients were transformed into a 3D data model suitable for the 3D printer. Our current activities involve various aspects of the printing process, material research and the implementation of the cell-seeding process. Different resorbable polymeric materials have been examined and used to fabricate heart valve scaffolds by rapid manufacturing. Human vascular cells attached to the scaffold surface should migrate additionally into the inner structure of the polymeric samples. The ultimate intention of our approach is to establish a heart valve fabrication process based on 3D rapid manufacturing and TE. Based on the computer tomographic data of a patient, a custom-made scaffold for a valve will be produced on a 3D printer and populated preferably by autologous cells. The long-term goal is to support the growth of a new valve by a 3D structure resorbed by the human body in the course of the growth process. Our current activities can be characterized as basic research in which the fundamental steps of the technical process and its feasibility are investigated. © The Author 2014. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery.

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