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Gitsopoulos T.K.,Greek National Agricultural Research Foundation | Vasilakoglou I.,Technological Educational Institute of Larissa | Tsoktouridis G.,Laboratory for the Conservation and Evaluation of Native and Floricultural Species
Biotechnology and Biotechnological Equipment | Year: 2013

The versatile rhizomatous terrestrial form of the perennial weed Persicaria amphibia is reported to cause severe infestations in potato crops, which are mainly cultivated as a monoculture in the area of Kato Nevrokopi located at the Greek-Bulgarian boundaries. This weed cannot be easily eradicated from potato crops in Northern Greece and therefore, the accurate taxonomic identification of P. amphibia has a high economical importance. The generic classification of P. amphibia has been unstable, mostly due to different morphological interpretations with other Persicaria species. Molecular studies based on DNA sequence data from the rbcL and matK plastid regions supported 100% homology with P. amphibia. Further analysis using the psbK-psbI, atpF-atpH, rpoC1 and rpoB plastid regions identified distinct differences with other Persicaria species suggesting the application of psbK-psbI and atpF-atpH as potential molecular markers for identification and phylogenetic analysis of Persicaria taxa. Morphology description and ecology characteristics concerning the terrestrial form of P. amphibia are provided. A multidisciplinary approach with both molecular and morphological information is described in this study and provides potential useful tools for the identification and taxonomy of Persicaria species. © Biotechnol. & Biotechnol. Source

Tsoktouridis G.,Alexander Technological Educational Institute of Thessaloniki | Tsoktouridis G.,Laboratory for the Conservation and Evaluation of Native and Floricultural Species | Koutinas N.,Alexander Technological Educational Institute of Thessaloniki | Osmantzikidis I.,Alexander Technological Educational Institute of Thessaloniki | And 6 more authors.
Journal of Environmental Protection and Ecology | Year: 2014

Advance in genomics and genetic research in eucalypts is increasing and becomes valuable in resolving problems related to the identification of Eucalyptus taxa, their genetic diversity, phylogeny and phylogeography. Numerous molecular studies have been proved extremely useful in DNA barcoding many Eucalyptus species and hybrids, aiming to determine the accurate genetic classification and origin of eucalypts. Fifteen species of Eucalyptus have been investigated to find genetic differences, using the clpP molecular marker of chloroplast DNA. A total of 390 bp of the clpP intergenic region were amplified by polymerase chain reaction (PCR) for 15 Eucalyptus species and nucleotide differences were determined. A conservative plastid region of the first 83 nucleotides and three variable regions were identified for the 46 Eucalyptus species included in our study. A phylogenetic tree has been constructed based on DNA sequences generated in this study and another 31 available at GenBank. Source

Tsoktouridis G.,Laboratory for the Conservation and Evaluation of Native and Floricultural Species | Grigoriadou K.,Laboratory for the Conservation and Evaluation of Native and Floricultural Species | Doua E.,Laboratory for the Conservation and Evaluation of Native and Floricultural Species | Nikolaidou A.,Directorate of Research | And 2 more authors.
Propagation of Ornamental Plants | Year: 2013

In vitro mass propagation was achieved for four diverse genotypes of the wild carnation Dianthus petraeus, originating from four different geographical areas in Greece. Adventitious shoot proliferation was induced on Murashige and Skoog (MS) medium supplemented with 0.5, 1, and 2 mg l-1 of TDZ in combination with 0, 0.1 and 0.5 mg l-1 of NAA, using explant material from in vitro maintained stock microshoots. Hyperhydricity of the proliferated microshoots was observed and overcome by transferring the hyperhydrous microshoots to full-strength MS medium without growth regulators for four weeks prior to in vivo acclimatization. The effect of plant growth regulators was investigated and the mean number of adventitious shoots was recorded throughout all the developmental stages of micropropagation. The supplement of 0.1 mg l-1 NAA in the growth medium is recommended for all D. petraeus genotypes but treatments with different amounts of TDZ produced variable amounts of adventitious shoots (~17-24) per explant, depending on the genotype. A significant number of proliferated shoots was observed after the exclusion of TDZ and NAA during the hyperhydricity reduction treatment and a well developed root system formed using either NAA or IBA. The regenerated plantlets with well-developed root and shoot system were successfully acclimatized in greenhouse and exterior nursery conditions. Source

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