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Kubica M.,Inflammation Research Center | Kubica M.,Ghent University | Hildebrand F.,VIB | Hildebrand F.,Laboratory for Molecular Bacteriology Rega Institute | And 17 more authors.
Experimental Dermatology | Year: 2014

Caspase-14, an important proteinase involved in filaggrin catabolism, is mainly active in terminally differentiating keratinocytes, where it is required for the generation of skin natural moisturizing factors (NMFs). Consequently, caspase-14 deficient epidermis is characterized by reduced levels of NMFs such as urocanic acid and 2-pyrrolidone-5-carboxylic acid. Patients suffering from filaggrin deficiency are prone to develop atopic dermatitis, which is accompanied with increased microbial burden. Among several reasons, this effect could be due to a decrease in filaggrin breakdown products. In this study, we found that caspase-14-/- mice show enhanced antibacterial response compared to wild-type mice when challenged with bacteria. Therefore, we compared the microbial communities between wild-type and caspase-14-/- mice by sequencing of bacterial 16S ribosomal RNA genes. We observed that caspase-14 ablation leads to an increase in bacterial richness and diversity during steady-state conditions. Although both wild-type and caspase-14-/- skin were dominated by the Firmicutes phylum, the Staphylococcaceae family was reduced in caspase-14-/- mice. Altogether, our data demonstrated that caspase-14 deficiency causes the imbalance of the skin-resident bacterial communities. © 2014 John Wiley & Sons A/S.

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