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Mar del Plata, Argentina

Crupkin A.C.,Laboratory Ecotoxicologia | Carriquiriborde P.,CONICET | Mendieta J.,CONICET | Panzeri A.M.,University of the Sea | And 6 more authors.
Pesticide Biochemistry and Physiology

Short-term responses at the antioxidant enzymatic systems, together with genotoxic effects were studied in the freshwater fish Australoheros facetus, exposed to endosulfan (ES) (0.02, 0.5, 5, 10μg/L) for 24h. Brain was the most responsive organ, showing inhibition of the enzymatic systems together with an increase of hydrogen peroxide (H2O2) content. Concentration-dependent inhibition was observed for superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) with IC25 values of 0.012, 0.017, 0.018μg/L, respectively. In liver, a similar behavior was observed for SOD with IC25 values of 2.22μg/L. In addition, increased thiobarbituric acid reactive substances (TBARs) at 5μg/L and H2O2 at 5 and 10μg/L were observed. No effects were evidenced on ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), GR and CAT activities. In gills, only H2O2 decreased at 0.5 and 5μg/L ES. Genotoxic effects were detected by the increase of the frequency of both, nuclear abnormalities (NA) at 0.02μg/L and micronucleus (MN) at 5μg/L. Environmentally realistic concentrations of ES exerted toxic responses in A. facetus, encouraging the further field validation of the observed pattern (tissue specificity, sensitiveness and concentration-response relationship) as a potential suit of biomarkers for assessing acute sublethal effects in A. facetus under short-term pulsed exposure to ES. © 2013 Elsevier Inc. Source

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