Langfang, China
Langfang, China

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Dong X.,Center Laboratory | Niu L.,Center Laboratory | Wang D.,Center Laboratory | He Y.,Center Laboratory
MATEC Web of Conferences | Year: 2015

Being applied widely, electromagnetic wave is closely related with our life. But this material has brought pollutions to the environment as well as influences on functions of organisms. In order to explore the influence of electromagnetic radiation on nervous system, this paper takes adult mice on 35th day as research objects, designs a water maze experiment and explores features of escaping latency of mice in the control group and in the group with radiation. In this research, methods of building a GHz TEM cell and a simulation model of mouse head with AutoCAD 2010 and XFDTD are provided, verifying that the simulation model meets the needs of the experiment. It concludes that the electromagnetic radiation causes memory deterioration of mice, and exerts its certain influence on nervous system. © Owned by the authors, published by EDP Sciences, 2015.

Chang Z.,Center Laboratory | Zhang Z.,Center Laboratory | Liu F.,Center Laboratory | Wang J.,Center Laboratory | And 5 more authors.
Chinese Journal of Endemiology | Year: 2014

Objective: To study the feasibility of DNA barcoding in identification of host animals with plague. Methods: Seven species were collected, including Lasiopodomys brandti, Spermophilus dauricus, Ochotona daurica, Phodopus campbelli, Allactaga sibirica, Meriones unguiculatus and Rhombomys opimus in the epidemic focus of Lasiopodomys brandti, Inner Mongolia, China. Liver was extracted and mitochondrial cytochrome oxidase subunit I gene (CO I) was analyzed. The GC content and genetic distance by Kimura-2-parameter(K2P) were calculated using MEGA5.0, and the phylogenetic trees were constructed with Neighbor-Joining (NJ) method. Results: Sixty-one CO I gene sequence fragments were barcoded, and each sequence was 657 bp in length. The GC% of Allactaga sibirica, Lasiopodomys brandti, Meriones unguiculatus, Ochotona daurica, Phodopus campbelli, Rhombomys opimus and Spermophilus dauricus were (46.945 ± 0.109)%, (43.836 ± 0.000)%, (40.313 ± 0.084)%, (47.514 ± 0.073)%, (41.838 ± 0.073)%, (40.335 ± 0.000)% and (39.467 ± 0.032)%, respectively, and the differences between them were significant(all P < 0.05), except the difference between Rhombomys opimus and Meriones unguicalatus (P > 0.05). The 1st codon GC contents of the seven species were (57.534 ± 0.000)%, (55.251 ± 0.000)%, (54.338 ± 0.000)%, (57.078 ± 0.000)%, (55.308 ± 0.057)%, (53.881 ± 0.000)% and (56.164 ± 0.000)%, respectively, and the differences between them were significant (all P < 0.05), except the difference between Lasiopodomys brandti and Phodopus campbelli (P> 0.05); the 3rd codon GC content of the seven species were (40.835 ± 0.328)%, (33.790 ± 0.000)%, (24.136 ± 0.253)%, (43.455 ± 0.218)%, (27.740 ± 0.207)%, (24.201 ± 0.000)% and (19.772 ± 0.097)%, respectively, and the differences were significant (all P < 0.05) among species, except the difference between Rhombomys opimus and Meriones unguicalatus (P > 0.05). The average intraspecific genetic distance (K2P) was 0.003 and the average interspecific genetic distance was 0.234 8. A NJ tree showed 7 major clusters. Conclusion: CO I sequencing of DNA barcoding can be used to identify host animals with plague in the epidemic focus of Lasiopodomys brandti, and can provide stronger evidences in identification of species based on morphology.

Ma Z.-D.,Center Laboratory | Liu Y.-X.,Center Laboratory | Li Y.-H.,Center Laboratory | Li J.-Y.,Center Laboratory
Chinese Journal of Endemiology | Year: 2013

Objective: To find out the cut-off value of rose bengal plate agglutination test(RBPT) in rapid diagnosis of brucellosis. Methods: From May to June 2009, 398 people who came to the outpatient department of Inner Mongolia Center for Endemic Disease Control and Research were diagnosed brucellosis by RBPT and tube agglutination test (SAT). Tube agglutination test as a gold standard, rose bengal plate agglutination test, its authenticity and reliability were evaluated. Results: Taking positive predictive value 100.0% as the selection standard, the cut-off value of RBPT was "++" which could be used to diagnose brucellosis. The sensitivity was 83.3% ; the specificity was 100.0% ; the Youden index was 0.832; and the compliance rate was 89.9%. Conclusion: The cut-off value "++" of RBPT to diagnosis brucellosis is worthy of clinical promotion.

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