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Oslo, Norway

Martin Caballero J.,Laboratory Animal Unit | Garzon A.,Cancer Vaccines Unit | Garzon A.,Azurebio S.L. | Gonzalez-Cintado L.,CSIC - National Center for Biotechnology | And 16 more authors.
PLoS ONE | Year: 2012

Cervical cancer is caused by persistent high-risk human papillomavirus (HR-HPV) infection and represents the second most frequent gynecological malignancy in the world. The HPV-16 type accounts for up to 55% of all cervical cancers. The HPV-16 oncoproteins E6 and E7 are necessary for induction and maintenance of malignant transformation and represent tumor-specific antigens for targeted cytotoxic T lymphocyte-mediated immunotherapy. Therapeutic cancer vaccines have become a challenging area of oncology research in recent decades. Among current cancer immunotherapy strategies, virus-like particle (VLP)-based vaccines have emerged as a potent and safe approach. We generated a vaccine (VLP-E7) incorporating a long C-terminal fragment of HPV-16 E7 protein into the infectious bursal disease virus VLP and tested its therapeutic potential in HLA-A2 humanized transgenic mice grafted with TC1/A2 tumor cells. We performed a series of tumor challenge experiments demonstrating a strong immune response against already-formed tumors (complete eradication). Remarkably, therapeutic efficacy was obtained with a single dose without adjuvant and against two injections of tumor cells, indicating a potent and long-lasting immune response. © 2012 Martin Caballero et al.

Midtlyng P.J.,Laboratory Animal Unit
Aquaculture Research | Year: 2011

Since 1987, the use of antibacterial drugs in Norwegian fish farming has been drastically reduced from about 48tonnes to approximately 1tonne annually, varying slightly from year to year. The 649kg active substance prescribed in 2007 corresponds to approximately 0.03% of the produced biomass being treated once with an antibacterial drug. This is an exceptional figure, particularly when compared with antibacterial drug use in terrestrial animal production or humans. It is therefore highly unlikely that this limited use of antibacterial drugs in farmed fish poses a significant risk to human medicine via the development of antibacterial drug resistance. Among the factors contributing to this favourable situation are: (a) a unique government-industry initiative adopted in the early 1990s to facilitate vaccination against classical furunculosis; (b) development of high-quality vaccines by the pharmaceutical industry; © the continuing predominance of vaccination strategies for disease control among fish farmers; (d) adoption of 'all-in-all-out' production systems, with mandatory fallowing periods between year classes; and (e) zoning and the spatial re-arrangement of marine production sites to minimize horizontal spread of infections. In Norway, targeted and flexible governance has allowed the rapid implementation of mass vaccination as well as zoo-sanitary measures. The limited use, however, also tends to limit the availability of licensed veterinary drugs in the marketplace. This creates a dilemma that should be addressed as a strategic issue for further long-term development of sustainable industrialized aquaculture. © 2011 Blackwell Publishing Ltd.

Ignjatovic D.,Vestfold Hospital | Aasland K.,Laboratory Animal Unit | Pettersen M.,Vestfold Hospital | Sund S.,Forde Central Hospital | And 3 more authors.
American Journal of Surgery | Year: 2010

Aim: To determine the effect of a single dose of bevacizumab on adhesion formation in the rat cecum abrasion model. Methods: The cecum and parietal peritoneum of 38 male Wistar rats were abraded to promote adhesion formation. The rats were randomized into 2 groups: group 1 received bevacizumab (2.5 mg/kg) intraperitoneally, and group 2 received saline. On day 30 animals were killed, adhesions scored, and histopathological samples taken. Results: There was no wound dehiscence; there were 2 incision hernias (5.3%), 1 per group. Thirty-seven animals developed adhesions (97.4%). Adhesion grade and severity scores were significantly different between groups 1 and 2 at 2.7:1.6 (P = .018) and 3.8:2.7 (P = .007), respectively. There was no difference in adhesion square area (27.7:25.0%; P = .16), location (P = 1.00), or number (2.1:1.3; P = .06). Histopathology confirmed the statistical difference between groups (P = .049), and a highly significant correlation between results was shown (r = .758; P = .0001). Conclusion: A single dose of intraperitoneal bevacizumab significantly reduces grade and severity of abdominal adhesions in the cecum abrasion rat model. © 2010 Elsevier Inc.

Aasland K.E.,Laboratory Animal Unit | Skjerve E.,Center for Epidemiology and Biostatistics | Smith A.J.,Laboratory Animal Unit
Laboratory Animals | Year: 2010

A large number of methods for obtaining blood from mice have been published. In our facility, saphenous venepuncture is considered the method of choice for bleeding mice and is believed to have a number of welfare advantages when performed correctly. The aim of this study was to compare levels of haemolysis and plasma glucose in blood samples obtained by saphenous venepuncture and tail vein incision. The results indicate that saphenous venepuncture is the preferred method.

Chen Y.,University of Oslo | Eraker-Aasland Hansen K.,Laboratory Animal Unit | Spasojevic M.,Vestfold Hospital | Naesgaard J.-M.,Vestfold Hospital | And 2 more authors.
Surgical Laparoscopy, Endoscopy and Percutaneous Techniques | Year: 2014

INTRODUCTION: Pain and adhesions represent the challenge in hernia surgery. AIM: To investigate mesh fixation and adhesion prevention with a collagen-fibrin sealant. MATERIALS AND METHODS: Twenty-seven male Sprague-Dawley rats were operated twice, to create and repair 2 ventral hernias. Mesh fixation was with collagen-fibrin sealant on 1 side (group I), whereas an additional peritoneal suture was added in group II. On day 60 animals were killed and mesh migration, integration and number, grade and location of adhesions noted. RESULTS: Migration occurred in 12 (44.4%) in group 1 and 3 (11.1%) in group 2, P=0.023. Adhesions developed to 18 (33.3%) meshes. There was no difference in adhesion grade or area for mesh center or edge between the groups (P=0.735 and P=0.829, respectively). Median adhesion grade for mesh center was 1 and edge 3 (range, 0 to 4), P=0.005 and P=0.001, respectively. Granuloma formation was noted in 8 (18.6%) animals; only with suture-fixed mesh. CONCLUSIONS: Mesh fixation with fibrin sealant is not satisfactory, however, adhesion prevention seems to be; adhesions to the edge of the mesh are most severe. © 2014 by Lippincott Williams and Wilkins.

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