Entity

Time filter

Source Type

San José de las Lajas, Cuba

Lobaina L.I.,Laboratorios Of Investigaciones Del Sida | Noa E.,Laboratorios Of Investigaciones Del Sida | Dubed M.,Laboratorios Of Investigaciones Del Sida | Navea L.M.,Laboratorios Of Investigaciones Del Sida
Biotecnologia Aplicada | Year: 2013

This work was aimed at validating the viral inactivation property during the storage step of the production process of Melagenina® Plus, which is a Cuban biological product for the treatment of vitiligo. The product was challenged at storage with high loads of four viral models, corresponding to three enveloped and one non-enveloped virus, two of them RNA and the other two DNA viruses: the human immunodeficiency virus type 1 (HIV-1), the bovine viral diarrhea virus (BVDV), the porcine herpes virus type 1 (PHV-1) and the canine parvovirus (CPV). The viral titer was determined using the Reed-Muench method based on the viral cytopathic effect, and reduction factors were calculated as the difference of viral loads at the beginning and the end of the step. Enveloped viruses were inactivated between days 1 to 3, and the enveloped virus (CPV) was achieved after 21 days. The viral load showed a very highly significant decrease (p < 0.0001), being conditioned to storage temperature (30 ± 5 °C) and ethanol concentration (71 % minimum). The reduction factors achieved on this step (1:5.0 log for HIV-1; 3.5 log for BVDV; 4.24 log for PHV-1 and 5.8 log for CPV) characterized the adequate level of safety of the Melagenina® Plus production process. Source


Lobaina L.I.,Laboratorios Of Investigaciones Del Sida | Noa E.,Laboratorios Of Investigaciones Del Sida | Dubed M.,Laboratorios Of Investigaciones Del Sida | Navea L.M.,Laboratorios Of Investigaciones Del Sida
Biotecnologia Aplicada | Year: 2013

This work was aimed at validating the viral inactivation property during the storage step of the production process of Melagenina® Plus, which is a Cuban biological product for the treatment of vitiligo. The product was challenged at storage with high loads of four viral models, corresponding to three enveloped and one non-enveloped virus, two of them RNA and the other two DNA viruses: the human immunodeficiency virus type 1 (HIV-1), the bovine viral diarrhea virus (BVDV), the porcine herpes virus type 1 (PHV-1) and the canine parvovirus (CPV). The viral titer was determined using the Reed-Muench method based on the viral cytopathic effect, and reduction factors were calculated as the difference of viral loads at the beginning and the end of the step. Enveloped viruses were inactivated between days 1 to 3, and the enveloped virus (CPV) was achieved after 21 days. The viral load showed a very highly significant decrease (p < 0.0001), being conditioned to storage temperature (30 ± 5 °C) and ethanol concentration (71 % minimum). The reduction factors achieved on this step (1:5.0 log for HIV-1; 3.5 log for BVDV; 4.24 log for PHV-1 and 5.8 log for CPV) characterized the adequate level of safety of the Melagenina® Plus production process. Source


Barthelemy L.L.,Laboratorios Of Investigaciones Del Sida | Zaldivar L.Y.M.,Laboratorios Of Investigaciones Del Sida | Romero E.N.,Laboratorios Of Investigaciones Del Sida | de Armas M.B.,Laboratorios Of Investigaciones Del Sida | And 4 more authors.
Revista Cubana de Investigaciones Biomedicas | Year: 2010

By the classic biological assays it has been possible to determine the use of HIV-1 strains co-receptor, which have been classified in R5, X4 or R5/X4, characteristic related to the NIS or IS phenotype and to the clinical course. By bio-information methods the amino acid changes in the region of V3 loop of the env using the co-receptor. In present paper are showed the results of a characterization study of eleven strains from HIV-1 infected subjects, five seropositive and six with a fast progression to AIDS. Authors compared the results of phenotype study conducted by two classic assays, use of co-receptors and ability to induce syncytia in MT2 and three bio-information methods, rule 11/25, point matrices in specific locations (PSSM) and the geno2pheno program. The five strains from asymptomatic seropositive patients coincided according all methods used as R5/NIS. Five of the six strains from fast progression patients were classified as R5/X4 and IS by biological assays; whereas two were classified as R5/X4 by Geno 2 Pheno, one by PSSM and all were pure X4 by the rule 11/25. The assays used in the study allowed us to characterize the isolated strains and to relate the phenotype to the infection course, thus, we considered that using biological assays or bio-information methods it is possible to conduct characterization studies, its usefulness depends on the pursued objectives. Source

Discover hidden collaborations