Santos N.,Centro Hospitalar Sao Joao |
Iraola V.,Laboratorios Leti |
Placido J.L.,Centro Hospitalar Sao Joao
European Annals of Allergy and Clinical Immunology | Year: 2014
Background. Tetranychus urticae is a phytophagus mite found in the leaves of numerous plants. High sensitization rates have been demonstrated, however, provocation tests have only been performed in an occupational setting. Objective. To assess accuracy of skin prick tests and clinical relevance of T. urticae sensitization by means of conjunctival provocation tests (CPT) in a population without occupational exposure and to evaluate possible environmental risk factors for T. urticae allergy. Methods. Patients ≥ 18 years old sensitized to T. urticae (n = 12) and a non-sensitized control group (n = 12) were invited to perform CPT with T. urticae and fulfill a questionnaire including demographic data, questions on environmental exposure to T. urticae and allergy symptoms/diagnosis. A single-blinded placebo-controlled CPT with T. urticae (Leti®) was performed with increasing concentrations (0.002, 0.02, 0.2 and 2 mg/mL) and considered positive if conjunctival hyperemia, palpebral edema or lacrimation were observed in the tested eye. Results. Of T. urticae sensitized patients (mean wheal 4.4±1.5 mm), 9 had a positive CPT, including 3 monosensitized. A good diagnostic accuracy was found for skin prick tests: AUC = 0.952, sensitivity = 100%, specificity = 80%, positive likelihood ratio = 5 and negative likelihood ratio = 0 for a 3 mm wheal. No differences were found between allergic and non-allergic subjects regarding atopy, allergic disease or farming activities. Conclusions. A high prevalence of allergy to Tetranychus urticae was found in the north of Portugal. Future studies with a larger number of patients are needed to evaluate its relation to clinical symptoms and the impact of environmental factors. © 2014, EDRA LSWR. All Rights Reserved.
Morales M.,Laboratorios LETI |
Lopez-Matas M.A.,Laboratorios LETI |
Moya R.,Laboratorios LETI |
Carnes J.,Laboratorios LETI
Food Chemistry | Year: 2014
Non-specific lipid-transfer proteins (nsLTPs) are a family of pan-allergens present in foods and pollen. However, sequence homology among them is limited. The objective of this study was to evaluate the IgE-mediated cross-reactivity between nsLTPs from different sources and evaluate the allergenic properties of LTPs from peach (Pru p 3) and pellitory (Par j 1/Par j 2), major fruit and pollen allergens. Both proteins were purified and characterised. Cross-reactivity studies among nsLTPs from different foods and pollens were performed by immunoblot inhibition using sera specific to peach or pellitory pollen. Cross-reactivity with Pru p 3 was observed in hazelnut, onion, corn, peanut and apple while in pollens, none of the extracts was inhibited with Par j 1/2. In conclusion, Pru p 3 did not inhibit LTPs from most fruits. Therefore, although Pru p 3 covers the largest number of epitopes, diagnosis with only this allergen may not detect all LTP sensitivities. © 2014 Elsevier Ltd. All rights reserved.
Chan W.T.,CSIC - Biological Research Center |
Balsa D.,Laboratorios LETI |
Espinosa M.,CSIC - Biological Research Center
FEMS Microbiology Reviews | Year: 2015
Type II (proteic) toxin-antitoxin (TA) operons are widely spread in bacteria and archaea. They are organized as operons in which, usually, the antitoxin gene precedes the cognate toxin gene. The antitoxin generally acts as a transcriptional self-repressor, whereas the toxin acts as a co-repressor, both proteins constituting a harmless complex. When bacteria encounter a stressful environment, TAs are triggered. The antitoxin protein is unstable and will be degraded by host proteases, releasing the free toxin to halt essential processes. The result is a cessation of cell growth or even death. Because of their ubiquity and the essential processes targeted, TAs have been proposed as good candidates for development of novel antimicrobials. We discuss here the possible druggability of TAs as antivirals and antibacterials, with focus on the potentials and the challenges that their use may find in the 'real' world. We present strategies to develop TAs as antibacterials in view of novel technologies, such as the use of very small molecules (fragments) as inhibitors of protein-protein interactions. Appropriate fragments could disrupt the T:A interfaces leading to the release of the targeted TA pair. Possible ways of delivery and formulation of Tas are also discussed. © FEMS 2015.
Laboratorios Leti | Date: 2014-08-27
Laboratorios Leti | Date: 2016-01-21
Laboratorios Leti | Date: 2010-12-28
Bleaching preparations and other substances for laundry use; cleaning, polishing, scouring and abrasive preparations; soaps, bath gels, skin creams, perfumery, essential oils, cosmetics, hair lotions; dentifrices; repairing balm for the skin and lips.
Laboratorios Leti | Date: 2010-02-16
Pharmaceutical and veterinary preparations, namely, pharmaceutical preparations for the treatment, prevention and/or diagnosis of parasitic diseases. Education services, namely, arranging and conducting of colloquiums, conferences, congresses, seminars, symposiums, workshops and/or any type of similar event in the field of parasitic diseases; organization of exhibitions for educational and scientific purposes in such field. Scientific research for medical purposes, namely, research and development of preparations for the treatment, prevention and/or diagnosis of parasitic diseases.
Laboratorios Leti | Date: 2010-09-14
Chemicals used in industry, science and photography, chemicals for use in agriculture, horticulture and forestry except fungicides, herbicides, insecticides and parasiticides; unprocessed artificial resins, unprocessed plastics; soil fertilizers; fire extinguishing compositions; tempering chemicals for use in soldering; chemical products for the fresh-keeping and preserving of food; tanning substances, namely, tanning agents for use in the manufacture of leather; adhesives used in industry. Bleaching preparations, detergents, soap and pre-soak for laundry use; cleaning, polishing, scouring and abrasive preparations; soaps; perfumery, essential oils, cosmetics, hair lotions; dentifrices. Pharmaceutical preparations for the treatment of allergies; sanitary preparations for medical use; dietetic food adapted for medical use, food for babies; medical plasters; materials for dressings, namely, bandages, gauze; material for stopping teeth and dental wax; all purpose disinfectants; preparations for destroying vermin; fungicides, herbicides.
Laboratorios Leti | Date: 2011-05-31
Chemical preparations for scientific or research purposes, diagnostic preparations for scientific or research use. Pharmaceutical and veterinary preparations, namely, preparations containing proteins for the treatment, prevention and/or diagnosis of parasitic diseases.
Laboratorios Leti | Date: 2012-01-31
Chemical preparations for scientific or research purposes, diagnostic preparations for scientific or research purposes. Pharmaceutical and veterinary products, namely, preparations for the treatment, prevention and/or diagnosis of parasitic pathologies. Scientific research for medical purposes, namely, research and development of preparations for the treatment, prevention and/or diagnosis of parasitic pathologies.