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Sanz J.C.,Laboratorio Regional Of Salud Publica | Sanz J.C.,CIBER ISCIII | Culebras E.,Hospital Clinico San Carlos | Rios E.,Hospital Clinico San Carlos | And 5 more authors.
Journal of Clinical Microbiology | Year: 2010

Pneumotest-Latex (Statens Seruminstitut) was evaluated for direct serogrouping of Streptococcus pneumoniae strains in clinical samples from patients with invasive disease. The technique was accurate to its level of discrimination for 62 of 67 clinical samples (92.5%). Pneumotest-Latex would be a useful alternative for direct serogrouping of pneumococci in clinical samples. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Source


Sanz J.C.,Laboratorio Regional Of Salud Publica | Rodriguez-Avial I.,Servicio de Microbiologia | Rios E.,Servicio de Microbiologia | Ramos B.,Laboratorio Regional Of Salud Publica | Cercenado E.,Hospital General Universitario Gregorio Maranon
Infection | Year: 2014

Purpose: The aim of this study was to describe the clinical and microbiological characteristics of recurrent invasive pneumococcal disease (RIPD) cases identified in the Region of Madrid between January 2007 and December 2011. Methods: Streptococcus pneumoniae serotyping was performed by Pneumotest-Latex and Quellung reaction. Molecular typing was carried out by pulsed-field gel electrophoresis (PFGE). A relapse was defined as any case of RIPD caused by strains with similar PFGE profile. Re-infections were defined by detection of recurrent episodes caused by strains with different PFGE patterns. Results: During the study period, 2,929 S. pneumoniae strains isolated from 2,858 patients with invasive pneumococcal disease (IPD) were studied. In 61 patients (2.1 %), 132 episodes of RIPD were detected (two episodes in 52 patients, three in 8 and four in 1). Twelve patients had relapses, 47 had re-infections and two had re-infections followed by relapses. Common risk factors to developing RIPD were HIV (42.6 %) and haematological malignancies (16.4 %). The most frequent serotypes were 8 (16 episodes) and 19A (15 episodes). Fourteen strains that were resistant to levofloxacin were also resistant to erythromycin. The proportion of strains co-resistant to erythromycin and levofloxacin was significantly higher in relapses (11/29) than in re-infections (3/103). Conclusions: The occurrence of repeated episodes of IPD in the same patient over the time is not an exceptional issue. Some underlying conditions that may favour these recurrences, mainly immunosuppression, need to be considered in patients having an episode of IPD. © 2013 Springer-Verlag. Source


Rodriguez-Avial I.,Servicio de Microbiologia Clinica | Ramos B.,Laboratorio Regional Of Salud Publica | Rios E.,Servicio de Microbiologia Clinica | Cercenado E.,Hospital General Universitario Gregorio Maranon | And 4 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2011

Among 1,349 Streptococcus pneumoniae invasive isolates, 45 (3.3%) were levofloxacin resistant. Serotype distribution was as follows: 8 (n = 32 isolates), 19A (n = 4 isolates), 7F (n = 3 isolates), 9V (n = 2 isolates), 10A (n = 1 isolate), 19F (n = 1 isolate), 6B (n = 1 isolate), and nontypeable (n = 1 isolate). Levofloxacin-resistant isolates had dual mutations in the gyrA and parC genes. Serotype 8 strains corresponded to a capsular switching of the Sweden15A-25 clone. Levofloxacin resistance was also detected among multiresistant (ST27619A, Spain9V-ST156, ST88 19F, and ST15426B) and among usually antibiotic- susceptible (Netherlands7FST191, ST120119A, and ST263910A) clones. Copyright © 2011, American Society for Microbiology. All Rights Reserved. Source


Rodriguez M.A.G.,Direccion General de Atencion Primaria | Gonzalez A.V.,Direccion General de Atencion Primaria | Gavin M.A.O.,Direccion General de Atencion Primaria | Martinez F.M.,Direccion General de Atencion Primaria | And 3 more authors.
Vaccine | Year: 2011

To ascertain the factors linked to invasive pneumococcal disease (IPD) caused by the different serotypes in the period 2007-2009, following the conjugate vaccine's inclusion in the childhood vaccination schedule, a total of 2013 IPD cases were reviewed. The mean annual incidence in this period was 10.74 cases per 100,000 inhabitans and the lethality was 8.8%. Overall serotype distribution displayed certain peculiarities, such as the high frequency of serotype 5. Serotype 3, male gender, sepsis and presence of risk factors were significantly associated with lethality. Vaccinated children under 5 years of age had a higher risk of disease due to serotype 19A. Serotype 8 was associated with the presence of underlying risk factors. © 2011 Elsevier Ltd. Source


Garcia-Comas L.,Subdireccion General de Promocion de la Salud Y Prevencion | Ordobas M.,Subdireccion General de Promocion de la Salud Y Prevencion | Sanz J.C.,Laboratorio Regional Of Salud Publica | Ramos B.,Laboratorio Regional Of Salud Publica | And 2 more authors.
Enfermedades Infecciosas y Microbiologia Clinica | Year: 2016

Introduction Seroprevalence surveys enable the level of endemicity of hepatitis A (HAV) to be assessed. The aim of this study was to estimate the seroprevalence (SP) antibody against HAV by age group, and compare it with those obtained in previous surveys. Methods Observational cross-sectional study. The target population consists of residents from 2 to 60 years old in the Community of Madrid. Two-stage cluster sampling was performed with stratification of first stage units. After signing the informed consent, a serum sample was extracted from each participant and sociodemographic data were collected by a questionnaire. Results SP antibodies to hepatitis A is 46.8% (95% CI 44.6 to 49.0). The SP increases with age. It is higher in the population from more endemic countries and people with less education and lower social class. In relation to the previous survey, SP increased in the population under 30 years old, and a decline after that age is observed. If only the autochthonous population and from countries with very low endemicity is observed, the increase is statistically significant in the 2-5 years age group. Conclusions Our region has a very low level of endemicity thus, following the recommendations of WHO, vaccination should be targeted at specific risk groups. © 2015 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. Source

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