Florianópolis, Brazil
Florianópolis, Brazil

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Oliveira S.Q.D.,Laboratorio Of Quimica Farmaceutica | Almeida M.T.R.D.,Laboratorio Of Quimica Farmaceutica | Maraslis F.,Laboratorio Of Quimica Farmaceutica | Silva I.T.,Laboratorio Of Virologia Aplicada | And 4 more authors.
Phytochemistry Letters | Year: 2012

Investigation of the crude ethanolic extract obtained from the aerial parts of Dodonaea viscosa led to the isolation of three new ent-labdane diterpenes (1-3). The structures were established on the basis of their ESI-MS, UV, IR and NMR spectral data. The crude extract, fractions and compounds were evaluated for in vitro antiviral activity against Herpes Simplex Virus type 1 (HSV-1). The crude extract showed promising antiherpes activity expressed by a selectivity index of 6.2. © 2012 Phytochemical Society of Europe.


Fongaro G.,Laboratorio Of Virologia Aplicada | Hernandez M.,Leon Institute of Technology | Hernandez M.,University of Valladolid | Garcia-Gonzalez M.C.,Leon Institute of Technology | And 2 more authors.
Food and Environmental Virology | Year: 2016

The use of propidium monoazide (PMA) coupled with real-time PCR (RT-qPCR or qPCR for RNA or DNA viruses, respectively) was assessed to discriminate infectious enteric viruses in swine raw manure, swine effluent from anaerobic biodigester (AB) and biofertilized soils. Those samples were spiked either with infectious and heat-inactivated human adenovirus-2 (HAdV-2) or mengovirus (vMC0), and PMA-qPCR/RT-qPCR allowed discriminating inactivated viruses from the infective particles, with significant reductions (>99.9 %). Then, the procedure was further assayed to evaluate the presence and stability of two non-cultivable viruses (porcine adenovirus and rotavirus A) in natural samples (swine raw manure, swine effluent from AB and biofertilized soils); it demonstrated viral inactivation during the storage period at 23 °C. As a result, the combination of PMA coupled to real-time PCR can be a promising alternative for prediction of viral infectivity in comparison to more labour-intensive and costly techniques such as animal or tissue-culture infectivity methods, and for those viruses that do not have currently available cell culture techniques. © 2016 Springer Science+Business Media New York

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