Studies on the feeding habits of Lutzomyia (Lutzomyia) longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae: Phlebotominae) populations from endemic areas of American Visceral Leishmaniasis in Northeastern Brazil
Afonso M.M.D.S.,Vetores das Leishmanioses Do Instituto Oswaldo Cruz |
Duarte R.,Laboratorio Of Pesquisa E Servicos Em Saude Publica |
Caranha L.,Laboratorio Of Vetores |
Rangel E.F.,Vetores das Leishmanioses Do Instituto Oswaldo Cruz
Journal of Tropical Medicine | Year: 2012
The aim of this study was to identify potential blood feeding sources of L. (L.) longipalpis specimens from populations in Northeastern Brazil, endemic areas of American Visceral Leishmaniasis (AVL) and its correlation with the transmission of L. (L.) i. chagasi. The ELISA technique was applied using bird, dog, goat, opossum, equine, feline, human, sheep, and rodent antisera to analyze 609 females, resulting in an overall positivity of 60. In all municipalities, females showed higher positivity for bird followed by dog antiserum and sand fly specimens were also positive for equine, feline, human, sheep, goat, opossum, and rodent antisera. The finding for 17 combinations of two or three types of blood in some females corroborates the opportunistic habit of this sand fly species. The results demonstrating the association between L. (L.) longipalpis and opossum suggest the need for further evaluation of the real role of this synanthropic mammal in the eco-epidemiology of AVL. Copyright © 2012 Margarete Martins dos Santos Afonso et al.
Wermelinger E.D.,Laboratorio Of Vetores |
Benigno C.V.,Laboratorio Of Vetores |
Machado R.N.M.,Laboratorio Of Vetores |
Cabello P.H.,Instituto Oswaldo Cruz IOC |
And 2 more authors.
Brazilian Journal of Biology | Year: 2012
This study observed the mosquito population in a rural eutrophised dam. Larvae of L3 and L4 stages and pupae were dipped out during twelve month collections and the reared to the adult stage for identification. The collections were done along nine metres from the edge of the dam divided in three parts (P1, P2 and P3), each part being 3 m long. P1 did not have vegetation (grass) along its edge, which would reach or sink into the water to promote some shade on the marginal water. A total of 217 adults of four species was identified with the following constancies and frequencies: Culex quinquefasciatus (Say, 1823) (83% and 40.6%), Anopheles (Nyssorhynchus) evansae (Brèthes, 1926) (92% and 26.7%), Anopheles (Nyssorhynchus) rangeli (Gabaldon, Cova Garcia and Lopez, 1940) (83% and 14.3%) and Culex nigripalpus (Theobald, 1901) (33% and 18.4%). C. quinquefasciatus, A. evansae, A. rangeli and C. nigripalpus were more frequent in the quarters Nov./Dec./Jan. (85.7%), May/June/July (75%), Aug./Sept./Oct. (29.4%) and Aug./Sept./Oct. (23.5%) particularly in the months of December (88.4%) Sept. tember (48.94), (38.3) and August (47.62) respectively. The presence of C. quinquefasciatus and the high incidence of Daphinia sp. and also the levels of Organic Nitrogen (0.28 mg/L) and of total Phosphorus (0.02 mg/L) are indications of the eutrophication of the dam. There was a difference regarding the total of Anopheles (A. avansae + A. rangeli) and Culex species (C. quinquefasciatus + C. nigripalpis) between P1 and P2 (χ2 = 0.0097), P1 and P3 (χ2 = 0.0005), but not between P2 and P3 (χ2 = 0.2045).The high C. quinquefasciatus constancy and frequency were confirmed to be a good biological indicator for a eutrophised environment and A. evansae showed a good potential for this environment. Vegetation can be an important factor for anopheline population dynamic also in eutrophic breeding sites.
Terzian A.C.B.,Laboratorio Of Pesquisa Em Virologia |
Mondini A.,Laboratorio Of Pesquisa Em Virologia |
Mondini A.,Laboratorio Of Vetores |
De Moraes Bronzoni R.V.,Laboratorio Of Pesquisa Em Virologia |
And 3 more authors.
Vector-Borne and Zoonotic Diseases | Year: 2011
Arboviruses are frequently associated with outbreaks in humans and represent a serious public health problem. Among the Brazilian arboviruses, Mayaro virus, Dengue virus (DENV), Yellow Fever virus, Rocio virus, Saint Louis Encephalitis virus (SLEV), and Oropouche virus are responsible for most of human cases. All these arboviruses usually produce undistinguishable acute febrile illness, especially in the acute phase of infection. In this study we investigated the presence of arboviruses in sera of 519 patients presenting acute febrile illness, during a dengue outreak in São José do Rio Preto City (São Paulo, Brazil). A multiplex-nested RT-polymerase chain reaction assay was applied to detect and identify the main Brazilian arboviruses (Flavivirus, Alphavirus, and Orthobunyavirus genera). The molecular analysis showed that 365 samples were positive to DENV-3, 5 to DENV-2, and 8 to SLEV. Among the positive samples, one coinfection was detected between DENV-2 and DENV-3. The phylogenetic analysis of the SLEV envelope gene indicated that the virus circulating in city is related to lineage V strains. These results indicated that during that large DENV-3 outbreak in 2006, different arboviruses cocirculated causing human disease. Thus, it is necessary to have an efficient surveillance system to control the dissemination of these arboviruses in the population. © Copyright 2011, Mary Ann Liebert, Inc. 2011.
Alevi K.C.C.,São Paulo State University |
Rodas L.A.C.,Superintendencia de Controle de Endemias |
Tartarotti E.,Federal University of Mato Grosso do Sul |
Azeredo-Oliveira M.T.V.,São Paulo State University |
Guirado M.M.,Laboratorio Of Vetores
Genetics and Molecular Research | Year: 2015
To complement the epidemiological data and assist in the prophylaxis of Chagas disease in the State of São Paulo, we examined entomological lifting conducted in 40 municipalities of the Western region of the state from 2004 to 2008, highlighted the main vector species in this region, and reanalyzed the cytogenetic characteristics of Rhodnius neglectus from 3 different Brazilian states (Formoso/GO, Frutal/MG, Guaíra/SP, and Pitangueiras/SP). The municipalities of Castilho and Santo Antônio do Acaranguá registered the highest relative amounts of notifications. The main species notified in Western São Paulo were Triatoma sordida and R. neglectus. We collected a large number of T. sordida in 2005 and noted the absence of notification of infected insects in 2008. We observed no variation in chromosomal characteristics of R. neglectus of different states. These data are complementary to the survey presented from 1990 to 1999, as the vector species were the same (T. sordida and R. neglectus), with emphasis on T. sordida. We corroborate the future colonization domiciliary initially proposed for T. sordida in the region and underscore the importance of vector control programs in the prophylaxis of Chagas disease. Furthermore, we observed that the populations of R. neglectus in Brazil showed no intraspecific variation and we corroborated the chromosomal patterns originally described. These data are important for understanding the evolution of these hematophagous insects, which are vectors of Chagas disease. © FUNPEC-RP.
The presence of culicidae species in medium-sized cities in the State of São Paulo, Brazil and the risk of west nile fever and other arbovirus infection [Presença de culicídeos em município de porte médio do estado de são paulo e risco de ocorrência de febre do nilo ocidental e outras arboviroses]
Dibo M.R.,Servico Regional 8 |
Dibo M.R.,Laboratorio Of Vetores |
de Menezes R.M.T.,Laboratorio Of Entomologia Medica |
Ghirardelli C.P.,Laboratorio Of Entomologia Medica |
And 2 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2011
Introduction: The objectives for this study were to measure the diversity of Culicidae species, describe their abundance and seasonal variation in São José do Rio Preto, SP, and discuss the risk of arbovirus infections. Methods: The collection of larval and adult mosquitoes was conducted monthly from 2006 to 2007 in an urban area and four sections of forested land. In the urban area, larvae were collected from sites where oviposition by Culex mosquitoes was most likely to occur. At two of the four sites in the forested land, adult mosquitoes were collected with the use of CDC traps at night, and a Nasci aspirator was used in the daytime at the two other collection sites. Results: In the urban area, 34 Culicidae species were identified out of a total sample of 8,683 specimens; of these specimens, 80.7% were Culex quinquefasciatus, 9.6% were Culex coronator, 3.2% were Aedes albopictus, and 1.1% were Ochlerotatus fluviatilis. The abundance of Cx. quinquefasciatus larvae was negatively related to rainfal. In the woods, 2,268 mosquitoes were collected, representing 10 genera and 46 species. The most abundant mosquito species were Aedeomyia squamipennis, Culex coronator, Culex (Mel.) Melanoconion section, Culex declarator, Ochlerotatus scapularis, Anopheles triannulatus, Culex bidens/interfor and Culex habilitator/pseudojhantinosoma. Conclusions: The abundance of Cx. quinquefasciatus in the urban area and the presence of other Culicidae species in urban areas and forested land point to the possibility of the transmission of West Nile virus and other arbovirus infections in São José do Rio Preto and other cities. Thus, the enacting of measures aimed at the surveillance of these arbovirus infections is essential.
Favaro E.A.,Laboratorio Of Virologia |
Dibo M.R.,Laboratorio Of Vetores |
Pereira M.,Diretoria de Combate a Vetores |
Chierotti A.P.,Laboratorio Of Vetores |
And 2 more authors.
Revista de Saude Publica | Year: 2013
OBJECTIVE: To evaluate the most productive types of properties and containers for Aedes aegypti and the spatial distribution of entomological indices. METHODS: Between December 2006 and February 2007, the vector's immature forms were collected to obtain entomological indices in 9,875 properties in the Jaguare neighborhood of Sao Jose do Rio Preto, SP, Southeastern Brazil. In March and April 2007, a questionnaire about the conditions and characteristics of properties was administered. Logistic regression was used to identify variables associated with the presence of pupae at the properties. Indices calculated per block were combined with a geo-referenced map, and thematic maps of these indices were obtained using statistical interpolation. RESULTS: The properties inspected had the following Ae. aegypti indices: Breteau Index = 18.9, 3.7 larvae and 0.42 pupae per property, 5.2 containers harboring Ae. aegypti per hectare, 100.0 larvae and 11.6 pupae per hectare, and 1.3 larvae and 0.15 pupae per inhabitant. The presence of yards, gardens and animals was associated with the presence of pupae. CONCLUSIONS: Specific types of properties and containers that simultaneously had low frequencies among those positive for the vector and high participation in the productivity of larvae and pupae were notidentified. The use of indices including larval and pupal counts does not provide further information beyond that obtained from the traditional Stegomyia indices in locations with characteristics similar to those of São José do Rio Preto. The indices calculated per area were found to be more accurate for the spatial assessment of infestation. The Ae. aegypti infestation levels exhibited extensive spatial variation, indicating that the assessment of infestation in micro areas is needed.