Burgos L.,Laboratorio Of Toxicidade Genetica |
Lehmann M.,Laboratorio Of Toxicidade Genetica |
de Andrade H.H.R.,Federal University of Rio Grande do Sul |
de Abreu B.R.R.,Laboratorio Of Toxicidade Genetica |
And 3 more authors.
Ecotoxicology and Environmental Safety
The water eutrophication process by phosphorus and nitrogen allows cyanobacteria blooms which promote, among other effects, the generation and release of the metabolite 2-methylisoborneol (2-MIB) in the environment. This substance has been shown to be recalcitrant to conventional water treatment, degrading water quality. Considering the limited number of studies on the biological effects of 2-MIB in eukaryotic organisms, the present study assessed the genotoxicity of 2-MIB using the in vitro comet assay and cytokinesis block-micronucleus (CBMN-Cytome) assay on Chinese Hamster Ovary (CHO) cells and the in vivo Drosophila melanogaster Somatic Mutation and Recombination Test (SMART). The results showed that 2-MIB (125, 250 and 500. μg/mL) was unable to induce gene and chromosome mutations or events associated with mitotic recombination in the SMART. Similarly, four different concentrations (7.5, 15, 30 and 60. μg/mL) of 2-MIB did not induce increments in frequencies of micronuclei, nuclear buds, and nucleoplasmatic bridges in the CBMN-Cytome assay. In the comet assay, the positive results were restricted to the highest dose, 60. μg/mL of 2-MIB. The results obtained may help evaluate the genotoxic profile of extracellular algal products. © 2013 Elsevier Inc. Source