Laboratorio Of Tossicologia

Alessandria del Carretto, Italy

Laboratorio Of Tossicologia

Alessandria del Carretto, Italy
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Bianchi V.,Laboratorio Of Tossicologia | Pacifici R.,Instituto Superiore Of Sanita | Palmi I.,Instituto Superiore Of Sanita | Pichini S.,Instituto Superiore Of Sanita | And 11 more authors.
Biochimica Clinica | Year: 2010

This document presents a comprehensive overview of biochemistry and physiology of transferrin (Tf) and carbohydrate-deficient transferrin (CDT) and on the role of CDT as alcohol abuse marker. It discusses the characteristics of the available analytical methodologies for CDT determination and finally presents a set of recommendations. CDT measurements should be performed only in accredited laboratories, having a quality system in place and regularly performing IQC and participating in EQAS. Unequivocal patient identification and a documented chain of custody are needed. Non hemolysed serum is the preferred sample and, if it has to be stored for more than 8 h, freezing at -20°C is recommended. Immunological methods are acceptable only as first level testing. A confirmation of positive findings with analytical methods based on different physicochemical principles is recommended. The result should be expressed as percentage of disialo-Tf on total Tf. Each laboratory should establish its own decision limit based on the characteristics of the analytical method used and on the scope for which the measurement is performed. The employed analytical method should be indicated in the final report.


Crespi V.,Laboratorio Of Tossicologia | Maio R.C.,Laboratorio Of Tossicologia | Veronesi G.,University of Insubria | Gianfagna F.,University of Insubria | And 3 more authors.
Medicina del Lavoro | Year: 2015

Background: Previous reports revealed poor performance in identifying drugs of abuse users through first-level workplace drug testing (WDT), based on urine samples. In a cross-sectional study, we evaluated: (i) the effect of creatinine normalization of drug values from diluted urine samples (creatinine levels ≤20 mg/dL) on the prevalence of drug users; (ii) the independent procedure-related predictors of positivity and dilution. Methods: Workers' urine samples were collected at the workplace or at our certified laboratory between 2008 and 2012. All samples were analysed for drugs of abuse by immuno-enzymatic method in our laboratory, according to the Italian WDT law. Detectable drugs of abuse concentrations lower than the positive cutoff values were normalized based on mean levels of urinary creatinine. Detectable concentrations of drugs were confirmed by GC/MS. Multivariate logistic regression was used to detect independent procedure-related predictors of positive and diluted urine samples. Results: Of the 3080 urine samples screened, 51 (1.7%) were found positive for some drugs of abuse (26 cannabinoids and 16 cocaine) and 116 (3.8%) were diluted. Seventeen out of 23 diluted urine samples with detectable concentrations of cannabinoids or cocaine were found positive after urine creatinine normalization and GC/MS confirmed both negative and positive results. This increased the percentage of positivity for cannabinoids and cocaine from 1.35% to 2.09% (+55%, p=0.0005), which is closer to the expected prevalence of drug users based on Italian self-reported surveys. Collection of samples in the laboratory was an independent predictor of positivity (OR=2.33, 95%CI 1.27-4.28) and diluted urine sample (OR=1.65, 95%CI 1.04-2.61). Conclusions: Efficacy of first-level WDT could be improved by well-controlled pre-Analytical procedures and urine creatinine normalization of detected concentrations of drugs of abuse.


Rota C.,Laboratorio Of Tossicologia | Biondi M.,Laboratorio Of Tossicologia | Trenti T.,Laboratorio Of Tossicologia
Clinical Chemistry and Laboratory Medicine | Year: 2012

Background: Aution Max AX-4030, a test strip analyzer recently introduced to the market, represents an upgrade of the Aution Max AX-4280 widely employed for urinalysis. This new instrument model can allocate two different test strips at the same time. In the present study the two instruments have been compared together with the usage of Uriflet 9UB and the recently produced Aution Sticks 10PA urine strips, the latter presenting an additional test area for the measurement of urinary creatinine. Methods: Imprecision and correlation between instruments and strips have been evaluated for chemical-physical parameters. Accuracy was evaluated for protein, glucose and creatinine by comparing the semi-quantitative results to those obtained by quantitative methods. The well-known interference effect of high ascorbic acid levels on urine glucose test strip determination was evaluated, ascorbic acid influence was also evaluated on protein and creatinine determination. Results: The two instruments have demonstrated comparable performances: precision and correlation between instruments and strips, evaluated for chemical-physical parameters, were always good. Furthermore, accuracy was always very good: results of protein and glucose semi-quantitative measurements resulted to be highly correlated with those obtained by quantitative methods. Moreover, the semi-quantitative measurements of creatinine, employing Aution Sticks 10PA urine strips, were highly comparable with quantitative results. Conclusions: 10PA urine strips are eligible for urine creatinine determination with the possibility of correcting urinalysis results for urinary creatinine concentration, whenever necessary and calculating the protein creatinine ratio. Further studies should be carried out to evaluate effectiveness and appropriateness of the usage of creatinine semi-quantitative analysis. © 2011 by Walter de Gruyter.


PubMed | Laboratorio Of Tossicologia
Type: Comparative Study | Journal: Clinical chemistry and laboratory medicine | Year: 2012

Aution Max AX-4030, a test strip analyzer recently introduced to the market, represents an upgrade of the Aution Max AX-4280 widely employed for urinalysis. This new instrument model can allocate two different test strips at the same time. In the present study the two instruments have been compared together with the usage of Uriflet 9UB and the recently produced Aution Sticks 10PA urine strips, the latter presenting an additional test area for the measurement of urinary creatinine.Imprecision and correlation between instruments and strips have been evaluated for chemical-physical parameters. Accuracy was evaluated for protein, glucose and creatinine by comparing the semi-quantitative results to those obtained by quantitative methods. The well-known interference effect of high ascorbic acid levels on urine glucose test strip determination was evaluated, ascorbic acid influence was also evaluated on protein and creatinine determination.The two instruments have demonstrated comparable performances: precision and correlation between instruments and strips, evaluated for chemical-physical parameters, were always good. Furthermore, accuracy was always very good: results of protein and glucose semi-quantitative measurements resulted to be highly correlated with those obtained by quantitative methods. Moreover, the semi-quantitative measurements of creatinine, employing Aution Sticks 10PA urine strips, were highly comparable with quantitative results.10PA urine strips are eligible for urine creatinine determination with the possibility of correcting urinalysis results for urinary creatinine concentration, whenever necessary and calculating the protein creatinine ratio. Further studies should be carried out to evaluate effectiveness and appropriateness of the usage of creatinine semi-quantitative analysis.

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