Seropédica, Brazil
Seropédica, Brazil

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Baldotto L.E.B.,Federal University of Viçosa | Baldotto M.A.,UFV Campus Florestal | Canellas L.P.,Laboratorio Of Solos | Bressan-Smith R.,Laboratorio Of Genetica E Melhoramento Of Plantas | Olivares F.L.,Laboratorio Of Biologia Celular E Tecidual
Revista Brasileira de Ciencia do Solo | Year: 2010

In vitro propagation of pineapple produces uniform and disease-free plantlets, but requires a long period of acclimatization before transplanting to the field. Quicker adaptation to the ex vitro environment and growth acceleration of pineapple plantlets are prerequisites for the production of a greater amount of vigorous, well-rooted planting material. The combination of humic acids and endophytic bacteria could be a useful technological approach to reduce the critical period of acclimatization. The aim of this study was to evaluate the initial performance of tissue-cultured pineapple variety Vitória in response to application of humic acids isolated from vermicompost and plant growth-promoting bacteria (Burkholderia spp.) during greenhouse acclimatization. The basal leaf axils were treated with humic acids while roots were immersed in bacterial medium. Humic acids and bacteria application improved shoot growth (14 and 102%, respectively), compared with the control; the effect of the combined treatment was most pronounced (147%). Likewise, humic acids increased root growth by 50%, bacteria by 81% and the combined treatment by 105%. Inoculation was found to significantly increase the accumulation of N (115%), P (112%) and K (69%) in pineapple leaves. Pineapple growth was influenced by inoculation with Burkholderia spp., and further improved in combination with humic acids, resulting in higher shoot and root biomass as well as nutrient contents (N 132%, P 131%, K 80%) than in uninoculated plantlets. The stability and increased consistency of the host plant response to bacterization in the presence of humic substances indicate a promising biotechnological tool to improve growth and adaptation of pineapple plantlets to the ex vitro environment.


Carneiro R.F.V.,UFPI | Martins M.A.,Laboratorio Of Solos | Vasquez H.M.,Laboratorio Of Zootecnia | Detmann E.,Federal University of Viçosa
Archivos de Zootecnia | Year: 2010

This work, carried out under greenhouse, had as objective to evaluate the response of the Stylosanthes to mycorrhizal inoculation and levels of phosphorus in soil no sterilized (natural conditions). A randomized blocks design was used in a 4 x 3 factorial scheme (four phosphorus levels: 0, 60, 120 and 240 mg/dm3 of soil; three microbiology treatments: control; inoculation with Glomus clarum and inoculation with native inoculum), with three repetitions. Two cuts of the aerial part were accomplished every 60 days. It was analyzed the production of dry matter of the aerial part and root, the accumulations of crude protein, P, K, Ca, Mg and S, percentage of mycorrhizal colonization and density of spores. The results demonstrated that the increase of the doses of P increased significantly the studied variables. Those results were evidenced by the presence of the mycorrhizal inoculation, with prominence for the presence of the species Glomus clarum, mainly for the first cut. Was estimated a reduction of 82.8 and 71.3% in the external requirement of P, for the establishment of the Stylosanthes in function of the inoculation with the Glomus clarum and native inoculum, respectively. In the second cut, effect of the microbiology treatments was verified, only for the accumulated amounts of Ca and Mg. The mycorrhizal colonization and the density of spores were not affected for the microbiology treatments.


Carneiro R.F.V.,CPCE UFPI | Martins M.A.,Laboratorio Of Solos | Araujo A.S.F.,CCA DEAS UFPI | Nunes L.A.P.L.,CCA DEAS UFPI
Archivos de Zootecnia | Year: 2011

This work, carried out under greenhouse, had for objective to evaluate the response of the intercropped Stylosanthes and Andropogon grass to arbuscular mycorrhizal inoculation and levels of phosphorus in soil no sterilized (natural conditions). A randomized blocks design was used in a 4 x 3 factorial scheme (four phosphorus levels: 0, 60, 120 and 240 mg dm-3 of soil) and three microbiology treatments (control; inoculation with Glomus clarum and inoculation with native inoculum) with three repetitions. Were accomplished two cuts of the aerial part, each 60 days. It was analyzed the production of dry matter of the aerial part and root, the accumulations of crude protein, P, K, Ca, Mg, and S, percentage of mycorrhizal colonization in both plants and density of spores. The results demonstrated that the increase of the doses of P increased significantly the studied variables. Those results were evidenced with the arbuscular mycorrhizal inoculation, specially for the species Glomus clarum, mainly for the first cut. The participation of the legume in the dry matter total of the mixture was increased by the arbuscular mycorrhizal inoculation in the smallest levels of P. The mycorrhizal colonization and the density of spores were not affected by the microbiology treatments.


Barroso A.A.F.,Laboratorio Of Solos | Gomes G.E.,Laboratorio Of Solos | Lima A.E.O.,Laboratorio Of Solos | Palacio H.A.Q.,IFCE Campus Iguatu | de Lima C.A.,Laboratorio Of Solos
Revista Brasileira de Engenharia Agricola e Ambiental | Year: 2011

The semiarid region is characterized as a region of high temperatures and as well as high temporal and spatial variability of rainfall. The deficiency of water resources is the main limiting factor in agricultural production. This study aimed to evaluate the quality of surface and groundwater for irrigation in South Central, Ceará, Brazil. Water samples were taken at 23 different points, eight representative of surface and underground waters and fifteen from the cities of Jucás, Cariús and Quixelô. The collection period was from April to June 2010 and analysis were performed at the Laboratory of Soil, Water and Plant Tissue of FCE Iguatu Campus. The parameters investigated were: calcium, magnesium, sodium, potassium, chloride, carbonate, bicarbonate, sulphate, electrical conductivity and sodium adsorption ratio. The software Qualigraf was used to perform the classification of samples through the Piper diagram to compare and classify different groups of waters in relation to the dominant ions. The results showed that the sources had higher risk of groundwater salinity and sodicity for use in irrigation and according to the Piper diagram sodic waters prevailed with 87.0% when considering the cations and the 78.3% as choride when anions were considered.


Pinto L.K.A.,Laboratorio Of Tecnologia Of Alimentos Lta | Martins M.L.L.,LTA | De Resende E.D.,LTA | Thiebaut J.T.L.,Laboratorio Of Engineering Agricola | Martins M.A.,Laboratorio Of Solos
Revista Brasileira de Fruticultura | Year: 2013

This study was carried out with the objective to evaluate the effect of controlled atmospheres containing different concentration of oxygen on the activity of β-galactosidase and pectinmethylesterase enzymes, on the skin color and pulp firmness in 'Golden' papaya. The fruits were kept for 36 days under the following controlled atmospheres: 1% of O2 and 0.03% CO2 with ethylene absorber, 3% of O2 and 0.03% of CO2 with ethylene absorber, 5% O2 and 0.03% of CO2 with ethylene absorber and environmental atmosphere without ethylene absorber. Relative humidity was set at the range 85-95% and temperature maintained at 13°C. Fruit stored under atmospheres of 1% of O2 and 0.03% CO2 had a β-galactosidase and pectimethylesterase activities delayed compared with the fruits storage under the other atmospheres evaluated. There was also, a delay in the development of color and pulp softening in fruits stored under atmospheres of 1% of O2 and 0.03% O2.


de Figueiredo C.C.,University of Brasilia | Resck D.V.S.,Embrapa Cerrados | Carneiro M.A.C.,Laboratorio Of Solos
Revista Brasileira de Ciencia do Solo | Year: 2010

Soil organic matter can be analyzed on the basis of the different fractions. Changes in the levels of organic matter, caused by land use, can be better understood by alterations in the different compartments. The aim of this study was to evaluate the effect of different management systems on the labile and stable organic matter of a dystrophic Red Latosol (Oxisol). The following properties were determined: total organic C and total N (TOC and TN), particulate organic C and particulate N (POC and PN), organic C and N mineral-associated (MOC and NM) and particulate organic C associated with aggregate classes (POCA). Eight treatments were used: seven with soil management systems and one with native Cerrado as a reference. The experiment was designed to study the dynamics of systems of tillage and crop rotation, alternating in time and space. The experimental design was a randomized block design with three replications. The soil samples were collected from five depths: 0-5, 5-10, 10-20, 20-30 and 30-40 cm. Changes in organic C by land use occurred mainly in the fraction of particulate organic matter (> 53 mm). Proper management of grazing promoted increased levels of particulate organic matter by association with larger aggregates (2-8 mm), demonstrating the importance of the formation of this aggregate class for C protection in pasture.


Bernardes T.G.,Instituto Federal Of Educacao | da Silveira P.M.,Laboratorio Of Solos | Carvalho M.T.M.,Laboratorio Of Solos | Madari B.E.,Laboratorio Of Solos | Carvalho M.C.S.,Laboratorio Of Solos
Revista Ceres | Year: 2015

New nitrogen fertilizers are available in the market actually, however, does not have results on the efficiency of the Cerrado conditions. With that objective of this study was to evaluate the effect of urea including stabilized and controlled release urea on yield of irrigated common beans (Phaseolus vulgaris L) in no-tillage system. The experiment was conducted in the winter crop, at Embrapa Arroz e Feijão, in Santo Antônio de Goiás, State of Goiás, Brazil. The experimental design was randomized blocks, with five replicates. Treatments consisted of five N sources (urea, urea + NBPT, urea + polymer, ammonium sulphate, and ammonium nitrate) and a control (without N) being applied 20 kg ha-1 of N at sowing and 80 kg ha-1 onf N in topdressing. We evaluated the chlorophyll content in leaves of common beans, the leaf N content and dry mass weight (MSPA) in the flowering of common beans, the number of pods per plant, number of grains per pod, mass of 100 grains, grain yield and final stand of the common beans. The sources of nitrogen fertilizer did not influence, leaf N content, the mass of MSPA and the relative chlorophyll index of common beans. The use of polymerized urea and urea with urease inhibitor, did not produce increases in the number of grains per pod, number of pods per plant, mass of 100 grains and common beans yield compared to traditional sources of N, urea, ammonium sulfate and ammonium nitrate.


Baldotto L.E.B.,State University of Norte Fluminense | Baldotto M.A.,Laboratorio Of Solos | Olivares F.L.,Laboratorio Of Biologia Celular E Tecidual | Viana A.P.,Laboratorio Of Genetica E Melhoramento Of Plantas | Bressan-Smith R.,Laboratorio Of Genetica E Melhoramento Of Plantas
Revista Brasileira de Ciencia do Solo | Year: 2010

Pineapple (Ananas comosus L. Merrill) in vitro propagation results in the production of a large quantity of healthy and homogeneous plantlets. Despite these advantages, the long time required for acclimatization makes this agricultural practice too costly. Growth acceleration of plantlets by inoculation with endophytic and epiphytic diazotrophic bacteria may be useful to reduce this period. The objectives of this study were to evaluate the potential of 20 diazotrophic bacteria strains in synthesizing indole, solubilizing Ca phosphate and Zn oxide and acting against the fungus Fusarium subglutinans f. sp. ananas, and subsequently, evaluate the performance of pineapple 'Vitória' propagated by tissue culture in response to the application of these isolates during the acclimatization period in a greenhouse. Shoot and root growth characteristics and leaf nutrient content of pineapple were evaluated. Results showed differences in the ability of the bacteria strains to synthesize indole, solubilize Ca phosphate and Zn oxide and act against Fusarium. Differences in the growth-promoting capacity of the shoot and root system of bacteria and leaf accumulation of N, P, K, Ca and Mg in pineapple were also shown. Inoculation with diazotrophic bacteria can promote the growth of plantlets during the acclimatization period, improving the adaptation of pineapple to the exvitro environment.


Ribeiro E.C.,Diretoria de Agrotecnologia DITEC | Carvalho D.F.D.,Federal Rural University of Rio de Janeiro | Santos L.A.D.F.,Federal Rural University of Rio de Janeiro | Guerra J.G.M.,Laboratorio Of Solos
Ciencia Rural | Year: 2016

The present study was carried out in order to evaluate the effects of irrigation depths applied through drip and presence/ absence of soil cover in onion yield, under agroecological farming. The experiment was conducted in Seropédica, RJ, Brazil, from May to September 2012. The experimental design was a randomized complete block with split-plot design with 10 treatments and 7 replications, characterized by the presence (+S) and absence (-S) of soil cover and five irrigation depths according to percentages of the crop evapotranspiration (ETc): 0, 22, 45, 75 and 100% (0, 155, 220.6, 320.5 and 372,7mm in +S condition and 0, 145.5, 207.6, 285, 351,4mm in -S condition). The irrigation influenced by second-order polynomial regression the total yield of bulbs, percentage distribution of bulbs in diameter classes and water use efficiency (WUE) in the -S condition, and in the linear regression the WUE in +S condition. The soil cover promoted an increase on the total bulb yield, WUE and the percentage of bulbs classified in classes of greater diameter. © 2016, Universidade Federal de Santa Maria. All rights reserved.


da Silva D.F.,State University of Montes Claros | Pegoraro R.F.,State University of Montes Claros | Medeiros A.C.,State University of Montes Claros | Lopes P.A.P.,State University of Montes Claros | And 2 more authors.
Pesquisa Agropecuaria Tropical | Year: 2015

The appropriate use of nitrogen fertilization and higher population densities increase the pineapple yield and can improve economic returns. This study aimed to evaluate the economic return and postharvest characteristics of irrigated Vitória pineapple under different nitrogen (N) sources and doses, as well as the increase in population density. A randomized blocks design with three replications was installed in a 2 x 5 x 4 factorial scheme, corresponding to two N sources (urea and cattle manure), five N doses (0 g plant−1, 5 g plant−1, 10 g plant−1, 15 g plant−1 and 20 g plant−1) and four densities (51,282 plants ha−1, 76,923 plants ha−1, 90,909 plants ha−1 and 126,984 plants ha−1). The N sources did not affect the pH, total titratable acidity and total soluble solids/total titratable acidity ratio (TSS/TTA). However the TSS content was higher in plants fertilized with cattle manure. The plants density did not affect the quality of postharvest fruit. The increase of N doses supplied by urea and cattle manure influenced only the total titratable acidity of the fruits. The combination of 20 g plant−1 of N-manure and a population of 126,984 plants ha−1 resulted in the maximum partial net income of R$ 132,096.48, however, the dose of 19.75 g plant−1 of N-urea, in the population of 119,686.00 plants ha−1, reached a partial net income of R$ 154,997.15. © 2015, Universidade Federal De Goias (UFG). All rights reserved.

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