Brasília, Brazil
Brasília, Brazil

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Cancellier L.L.,Federal University of Lavras | Afferri F.S.,Laboratorio Of Sementes | de Carvalho E.V.,Programa de Mestrado em Producao Vegetal | Dotto M.A.,Programa de Mestrado em Producao Vegetal | Leao F.F.,Federal University of Tocantins
Revista Ciencia Agronomica | Year: 2011

The objective of this study was to evaluate the nitrogen efficiency use and phenotypic correlation of 24 maize populations in Tocantins. Two experiments were conducted, each corresponding to a level of fertilization (high and low N) planted on 21/11/2009, treatments consisted of 24 populations obtained from the Maize Breeding Program of Federal University of Tocantins and a commercial variety, BR 106, used as control. The experimental design in both experiments was randomized blocks with two replicates, and evaluated 100 seeds weight, hectoliter weight, yield and nitrogen use efficiency according to the methodology by Fischer et al. (1983) and Moll et al. (1982). The genotypes 12-6; 25-5; 35-5; 12-2; 12-3 and 1-5 formed the group of genotypes of higher yields, independent of soil N, with averages ranging from 6,348 to 5,774 kg ha-1. By the methodology of Moll was possible to differentiate populations for nitrogen use efficiency in environment of low availability of soil N. The characteristics evaluated were influenced by different levels of nitrogen, except for 100 seeds weight. The populations studied differ itself in all characteristics, indicating variability. Moll index was significantly associated with all characteristics studied except for the Fischer index, indicating possible to obtain genotypes efficient in nitrogen use by selection of these characteristics.

Souza E.R.,São Paulo State University | Ribeiro V.G.,Bahia State University | Dantas B.F.,Laboratorio Of Sementes | Filho J.M.P.L.,Laboratorio Of Sementes
Revista Brasileirade Ciencias Agrarias | Year: 2013

The objective of this study was to evaluate the variation of carbohydrates, proteins and free amino acids in vine leaves in cv. Italy under different levels in different phases phenological defoliation. The experimental design was randomized blocks in factorial scheme 4 (5, 10, 15 and 20 sheets left in the industry) X 4 (phenological phases at 60, 72, 84 and 106 days after pruning) and three replications. We selected leaves were exposed to sunlight, fully extended, opposite the bunch, with no signs of senescence and healthy for the indirect measurement of chlorophyll (chlorophyll index) in SPAD readings using portable chlorophyll meter SPAD-502. The leaves were subjected to quantitative analysis of proteins, amino acids, starch, total sugars, reducing sugars and sucrose. The starch content was high in the initial softening of berries and sheets 15 left on the field of production and low at the start of the ripening of fruits. 15 The amount of leaves left on the branches of production in grapevine cv. Italy is recommended, therefore, these plants showed greater efficiency in both transmission and reserve accumulation and this accumulation was influenced by the phenological stage of grapevine.

Inglis P.W.,Laboratorio Of Genetica Vegetal | Ciampi A.Y.,Laboratorio Of Genetica Vegetal | Salomao A.N.,Laboratorio Of Sementes | Costa T.S.A.,Laboratorio Of Quimica Of Produtos Naturais | Azevedo V.C.R.,Laboratorio Of Genetica Vegetal
Genetics and Molecular Biology | Year: 2014

Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the microgravity-treated seedlings compared to terrestrially germinated controls. Suppression subtractive hybridization of cDNA and EST analysis were used to detect differential gene expression in the microgravity-treated seedlings in comparison to those initially grown in normal gravity (forward subtraction). Despite their return to, and growth in normal gravity, the subtracted library derived from microgravity-treated seedlings was enriched in known microgravity stress-related ESTs, corresponding to large and small heat shock proteins, 14-3-3-like protein, polyubiquitin, and proteins involved in glutathione metabolism. In contrast, the reverse-subtracted library contained a comparatively greater variety of general metabolism-related ESTs, but was also enriched for peroxidase, possibly indicating the suppression of this protein in the microgravity-treated seedlings. Following continued growth for 30 days, higher concentrations of total chlorophyll were detected in the microgravity-exposed seedlings. © 2014, Sociedade Brasileira de Genética. Printed in Brazil.

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