PubMed | University of Bari, Agenzia Regionale Protezione e Ambiente, Fondazione IRCCS Policlinico S. Matteo, Laboratorio Of Sanita Pubblica and 4 more.
Type: Journal Article | Journal: Diagnostic microbiology and infectious disease | Year: 2016
Legionella quantification in environmental samples is overestimated by qPCR. Combination with a viable dye, such as Propidium monoazide (PMA), could make qPCR (named then vPCR) very reliable. In this multicentre study 717 artificial water samples, spiked with fixed concentrations of Legionella and interfering bacterial flora, were analysed by qPCR, vPCR and culture and data were compared by statistical analysis. A heat-treatment at 55 C for 10 minutes was also performed to obtain viable and not-viable bacteria. When data of vPCR were compared with those of culture and qPCR, statistical analysis showed significant differences (P < 0.001). However, although the heat-treatment caused an abatement of CFU/mL 1 to 1 log10 unit, the comparison between untreated and heat-treated samples analysed by vPCR highlighted non-significant differences (P > 0.05). Overall this study provided a good experimental reproducibility of vPCR but also highlighted limits of PMA in the discriminating capability of dead and live bacteria, making vPCR not completely reliable.
Epis S.,University of Milan |
Epis S.,University of Camerino |
Matinato C.,Laboratorio Of Sanita Pubblica |
Gentili G.,Laboratorio Of Sanita Pubblica |
And 3 more authors.
Mycologia | Year: 2010
Amanita phalloides, Lepiota cristata, Lepiota brunneoincarnata and Inocybe asterospora are among the most important species responsible of mushroom poisoning in northern Italy. A real time PCR method for the identification of samples containing DNA from each of these species was developed. To test specificity all protocols were applied on DNA extract- ed from various mushroom species; sensitivity was assessed performing serial dilutions on all samples; versatility of the protocols was evaluated performing tests on DNA extracted from different matrices. The protocols showed high sensitivity (32 ng dried mushroom), high specificity and sensitive detection of DNA extracted from difficult samples, including pasta with mushroom, cooked mushrooms and gastric aspirates. © 2010 by The Mycological Society of America.
Fortes C.,Instituto Dermopatico dellImmacolata |
Mastroeni S.,Instituto Dermopatico dellImmacolata |
Pilla M.A.,Instituto Dermopatico dellImmacolata |
Antonelli G.,Instituto Dermopatico dellImmacolata |
And 2 more authors.
Food and Chemical Toxicology | Year: 2013
Concerns about pesticide exposure through food consumption have increased during the past several years. Pyrethroids are applied as insecticides throughout the world. Human metabolism of pyrethroids results in urinary metabolites that are suitable for biological monitoring. The objective of our study was to investigate the relation between food consumption and urinary levels of 3-phenoxybenzoic acid (3-PBA), a general metabolite of pyrethroids, in a non-occupational exposed adult population from the IDI-IRCCS, Rome, Italy. Information on socio-demographic characteristics, smoking, diet and self-reported household pesticide exposure was collected. Urinary 3-PBA level of each subject was measured and adjusted by urinary creatinine. We found that people consuming both raw and cooked vegetables five times weekly or more had higher mean levels of 3-PBA in urine (1.03 μg/g creatinine versus 0.52 μg/g creatinine; p= 0.009 and 0.99 μg/g creatinine versus 0.58 μg/g creatinine; p= 0.01, respectively) than subjects consuming less than five times weekly. In a multivariate model, after adjusting for age, sex, BMI, smoking and household insecticide exposure, high intake of raw vegetables (OR: 5.31; 95%CI: 1.32-21.3) and high intake of cooked vegetables, in particular cruciferous (OR: 4.67; 95%CI: 1.07-20.5) and leafy vegetables (OR: 6.88; 95%CI: 1.50-31.7), were associated with high urine 3-PBA levels (≥0.70 μg/g creatinine). The results of this study suggest that part of the variation in pyrethrois intake is explained by vegetable intake. © 2012 Elsevier Ltd.
Dose and effect biomarkers in the diagnosis of asbestos-related diseases and health surveillance of exposed workers [Gli indicatorl biologici di dose ed effetto nella diagnostica delle patologie asbesto-correlate e nella sorveglianza sanitaria degli ex-esposti]
Montomoli L.,University of Siena |
Romeo R.,University of Siena |
Sisinni A.G.,University of Siena |
Serio A.C.,University of Siena |
And 5 more authors.
Giornale Italiano di Medicina del Lavoro ed Ergonomia | Year: 2012
The aim of the study was to assess the reliability of the indicators of dose and effect in the health monitoring of asbestos exposed workers. In 49 cases out of 158 studied workers (31%) asbestos-related diseases were diagnosed following ATS criteria (2004). Using nonparametric statistical methods (permutation tests) 6 variables were analyzed with respect to asbestos-related diseases and working sectors, demonstrating a difference in the concentration of amphiboles (p <0.01), greater in patients with asbestosis and workers involved in asbestos removal from railway carriages. There was not a correlation between mesothelin and amphiboles, chrysotile and total fibers concentrations (Spearman test). © PI-ME, Pavia 2012.
Pinto I.,Laboratorio Of Sanita Pubblica |
Stacchini N.,Laboratorio Of Sanita Pubblica
Giornale Italiano di Medicina del Lavoro ed Ergonomia | Year: 2013
Whole Body Vibration (WBV) is the shaking or jolting of the human body through a supporting surface, usually a seat or floor. The risk from vibration is related to the overall time the operator or driver is exposed to the vibration and the number of shocks and jolts they experience each day. In the 27 countries of Europe the EC Physical Agents Directive, effective 6th July 2010, requires all employers to control exposure to a number of hazards including noise and vibration. The EC Vibration Directive sets out regulations for the control of health and safety risks from the exposure of workers to hand arm vibration (HAV) and whole body vibration (WBV) in the workplace. The maritime sector needs to comply: high exposure WBV levels can be found when operating RIBs and High Speed Craft. Marine sectors affected by Whole Body Vibration (WBV) include military, search & rescue, government agencies, local authorities, police, water sports, oil & gas, thrill ride, charter and all organisations operating boats, RIBs (Rigid Inflatable Boat) and High Speed Craft. Port machinery operators can be exposed to whole-body vibration (WBV). Fork lifts and mobile crane drivers can be exposes to relevant WBV: whole-body vibration measurements carried out in several italian ports indicates that around half of the machines would expose operators to vibration that would exceed the exposure action value of the Physical Agents (Vibration) Directive in less than 4 hours. Therefore it is be necessary to implement risk reduction measures, health surveillance training, and minimisation of the vibration exposure for maritime and port workers, that are illustrated in the present paper.
Cianti L.,UF Sanita Pubblica Veterinaria |
Lorini C.,University of Florence |
Santomauro F.,University of Florence |
Bavazzano P.,University of Florence |
And 3 more authors.
Italian Journal of Food Science | Year: 2013
The purpose of this study is to define a new system for assessing the freshness of marine bony fish based on measurable parameters. 151 fish were analysed to determine the concentrations of total volatile basic nitrogen, trimethylamine N-oxide, trimethylamine and malondialdehyde. The results of the determinations were included in an algorithm to calculate the value of the Freshness Index (FI). The most appropriate threshold value of FI that can distinguish fresh from not fresh fish was 0.33 (sensitivity 95.6%, specificity 73.6%). The results demonstrate the possibility of using the index for the evaluation, at low-cost, of consignments of fresh or presumed fresh fish both in the phase of official control and in self-verification systems.
Scapellato M.L.,University of Padua |
Aprea M.C.,Laboratorio Of Sanita Pubblica |
Moretto A.,University of Milan |
Bartolucci G.B.,University of Padua |
Manno M.,University of Naples Federico II
Giornale Italiano di Medicina del Lavoro ed Ergonomia | Year: 2013
Occupational exposure limits and guideline values for the general population proposed for benzene by several international bodies are discussed and compared with the Italian and EU occupational limit values, taking also into account the criteria used for their derivation. Benzene is an environmental pollutant, and the EU guideline value for ambient air is 5 μg/m3, based on carcinogenic risk. Presently, occupational exposures are greatly reduced, and in many instances close to those of the general population. Consequently, it does not seem to be appropriate to maintain the Italian and the EU occupational exposure limits of 1 ppm (3.2 mg/m3), which are inconsistent with the ALARA principle and not justified by technological constraints. It should be pointed out that, in any case, preventive interventions should be carried out, beyond the compliance with the established limit values, in order to ensure the lowest exposure and by carrying out biological monitoring as a tool to verify the appropriateness of risk management measures.