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Gonzalez A.M.,ADSG Vacasan | Arnaiz I.,Laboratorio Of Sanidad Y Produccion Animal Of Galicia Lasapaga | Yus E.,University of Santiago de Compostela | Sanjuan M.L.,University of Santiago de Compostela | And 2 more authors.
Cattle Practice | Year: 2014

The aim of the paper was to determine the long-term responses in dairy cattle herds after vaccination with inactivated bovine viral diarrhoea (BVD) vaccines with regard to antibodies against p80 protein, as detected by ELISA, in bulk tank milk samples. Thirty-three dairies that were going to be vaccinated for the first time with inactivated vaccines were followed during a 3-year period. During this follow-up, bulk tank milk samples were collected at the time of first vaccination and then every six months. Samples were analysed with a commercial ELISA based on the detection of antibodies against the p80 protein of the virus. Results indicated that the level of antibodies increased with time after vaccination and consequently there is an impact of immunisation on bulk tank milk antibody results. Vaccination status of the farms should be taken into consideration when interpreting these tests. Source


Gonzalez A.M.,ADSG Vacasan | Arnaiz I.,Laboratorio Of Sanidad Y Produccion Animal Of Galicia Lasapaga | Yus E.,University of Santiago de Compostela | Eiras C.,Laboratorio Of Sanidad Y Produccion Animal Of Galicia Lasapaga | And 2 more authors.
Veterinary Journal | Year: 2014

The aim of the present study was to determine the serological response of heifers after vaccination with two inactivated bovine viral diarrhoea virus (BVDV) vaccines by means of various ELISA tests. Three dairy farms were selected from the Galicia region of Spain. In each herd, a batch of heifers to be vaccinated for the first time was selected and followed for 15. months. Heifers from farm 1 (n= 25) were vaccinated with Vaccine A, whereas heifers from farm 2 (n= 16) were vaccinated with Vaccine B. Heifers from farm 3 (n= 17), where no BVDV vaccines were used, acted as controls. Blood samples were analyzed periodically for BVDV antibodies, using five commercial ELISAs, based on BVDV p80 antigen or whole virus.At the end of the study, none of the animals vaccinated with Vaccine A seroconverted according to p80 antibody status, whereas up to 80% tested positive by ELISA against whole virus antigen. For the animals vaccinated with Vaccine B, 2/16 animals seroconverted according to p80 antibody ELISAs, whereas all had seroconverted according to the ELISA against whole virus antigen. In most cases, based on the use of ELISAs to detect specific antibodies against the p80 protein, at 15. months post-vaccination with inactivated BVDV vaccines the responses did not seem to interfere with detection of antibody to BVDV infection. However, the finding of a small proportion of vaccinated animals seropositive against BVDV p80 antigen suggests that antibodies that interfere with diagnosis of BVDV infection within the herd could exist, even when using p80 ELISAs. © 2013 Elsevier Ltd. Source

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