Laboratorio Of Salud Publica
Laboratorio Of Salud Publica
Gutierrez L.A.,University of Antioquia |
Gomez G.F.,University of Antioquia |
Gonzalez J.J.,Laboratorio Of Salud Publica |
Castro M.I.,Laboratorio Of Salud Publica |
And 3 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2010
Anopheles darlingi is an important vector of Plasmodium spp. in several malaria-endemic regions of Colombia. This study was conducted to test genetic variation of An. darlingi at a microgeographic scale (approximately 100 km) from localities in Córdoba and Antioquia states, in western Colombia, to better understand the potential contribution of population genetics to local malaria control programs. Microsatellite loci: nuclear white and cytochrome oxidase subunit I (COI) gene sequences were analyzed. The northern white gene lineage was exclusively distributed in Córdoba and Antioquia and shared COI haplotypes were highly represented in mosquitoes from both states. COI analyses showed these An. darlingi are genetically closer to Central American populations than southern South American populations. Overall microsatellites and COI analysis showed low to moderate genetic differentiation among populations in northwestern Colombia. Given the existence of high gene flow between An. darlingi populations of Córdoba and Antioquia, integrated vector control strategies could be developed in this region of Colombia. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.
Calizaya-Anco J.,Private University of Tacna |
Avendano-Caceres M.,Laboratorio Of Salud Publica |
Delgado-Vargas I.,Laboratorio Of Salud Publica
Revista Peruana de Medicina Experimental y Salud Publica | Year: 2013
In order to evaluate the quality of the water of the Locumba river, Tacna (Peru), water samples were taken from ten stations located along the Locumba river basin, during six periods in an annual cycle. The diversity and number of diatoms was also evaluated, together with eleven physiochemical parameters in order to determine the degree of water contamination. We found that as the basin advanced down the mountain, the diversity of diatoms decreased from 2.37 bits cell-1 to 0.71 bits cell-1 and the gradient of contaminants increased. In addition to this increase, the number of species tolerant to high levels of environmental disturbance rose. An increase in all physiochemical parameters used to evaluate the degree of contamination was observed. These results suggest that diatoms can be adequate bioindicators when evaluating the quality of water in this basin.
Gonzalez-Parra G.,University of Los Andes, Venezuela |
Arenas A.J.,University of Córdoba, Colombia |
Aranda D.F.,Laboratorio Of Salud Publica |
Segovia L.,Coordinacion de Epidemiologia de la Corporacion de Salud
Spatial and Spatio-temporal Epidemiology | Year: 2011
The 2009 swine flu pandemic was a global outbreak of a new strain of H1N1 influenza virus and there are more than 14,000 confirmed deaths worldwide. The aim of this paper is to propose new mathematical models to study different dynamics of H1N1 influenza virus spread in selected regions around the world. Spatial and temporal elements are included in these models to reproduce the dynamics of AH1N1/09 virus. Different models are used since H1N1 influenza virus spread in regions with different contact structures are not the same. We rely on time series notifications of individuals to estimate some of the parameters of the models. We find that, in order to reproduce the time series data and the spread of the disease, it is convenient to suggest spatio-temporal models. Regions with only one wave are modeled with the classical SEIR model and regions with multiple waves using models with spatio-temporal elements. These results help to explain and understand about potential mechanisms behind the spread of AH1N1 influenza virus in different regions around the world. © 2011 Elsevier Ltd.
Maestre-Serrano R.,Laboratorio Of Salud Publica |
Maestre-Serrano R.,Simón Bolívar University of Colombia |
Eyes-Escalante M.,University of the Atlantic
Boletin de Malariologia y Salud Ambiental | Year: 2012
The department of Atlántico in Colombia is considered non-endemic for Chagas disease, however there are risk factors associated with the presence of vector species. According to the last record of distribution of triatomine species in Colombia, this department only reported the presence of Triatoma maculata. The objective of this article a is to update the presence and distribution of triatomine species in urban and rural areas in Atlántico-Colombia. A retrospective study was performed based on Atlántico's records of triatomines between the years 2003 to 2010. During the period studied the following species were recorded: Panstrongylus geniculatus in the municipalities of Piojó, Tubará, Puerto Colombia, Barranquilla, Soledad and Luruaco; Eratyrus cuspidatus in the municipalities of Piojó and Tubará and T. maculata in Puerto Colombia. These results broaden the distribution of triatomines in Atlántico department and Colombian Caribbean region.
Rojas L.J.,University of Los Andes, Colombia |
Jaramillo C.A.,University of Los Andes, Colombia |
Mojica M.F.,University of Los Andes, Colombia |
Escalante M.P.,Laboratorio Of Salud Publica |
Delgado P.,University of Los Andes, Colombia
Epidemiology and Infection | Year: 2012
Human adenoviruses (HAdV) cause a broad spectrum of diseases including acute respiratory infection (ARI), and are responsible for 5% of cases requiring hospitalization in children aged <5 years in Colombia; however, little is known about the circulating types, partly due to the lack of reliable typing tests. In order to evaluate a VA gene PCR-sequencing approach for identification of HAdV circulating types in a Colombian population, 52 nasopharyngeal aspirates/swabs from children with ARI were processed. After a BLAST analysis, matches with species B (41/48, 8542%), C (6/48, 125%), and D (1/48, 208%) were found; and at the type level, type 3 (22/48, 4583%) was the most frequent. This initial effort to expand our knowledge about the molecular epidemiology of HAdV circulating in Bogota, Colombia, showed that HAdV-B was the predominant circulating species in the study period and reports, for the first time in Colombia, the presence of HAdV-D in a respiratory sample. © Copyright Cambridge University Press 2011.
PubMed | University of Buenos Aires, Programa Nacional de Chagas and Laboratorio Of Salud Publica
Type: Journal Article | Journal: Cadernos de saude publica | Year: 2017
Residual insecticide spraying still is the main tool used to suppress house infestations with Chagas disease vectors. While manual compression sprayers (MCS) have traditionally been used in Latin America, Mendozas vector control program from Argentina introduced the use of a modified motorized vehicle-mounted sprayer (VMS) with apparent advantages over MCS. We conducted a randomized intervention trial to evaluate the effectiveness and selected components of the performance of MCS and VMS. We assessed house infestation by Triatoma infestans in 76 previously-infested houses at 0, 1, 4 and 12 months postintervention. Infestations were reduced substantially, with no significant differences between treatments. End-point infestations were restricted to peridomiciles. Although VMS required less time to complete the house spraying than MCS, both treatments had similar performance and did not suppress infestations completely. The main relative advantages of VMS were a reduced physical effort, especially under harsh field conditions, and potential gains in spray coverage per unit of time.
Patino Reyes N.,National University of Colombia |
Duarte Portocarrero E.,Laboratorio Of Salud Publica
One Century of the Discovery of Arsenicosis in Latin America (1914-2014): As 2014 - Proceedings of the 5th International Congress on Arsenic in the Environment | Year: 2014
In Bogota, Colombia, the District Department of Health ensures potable water consumption, and performs special tests for arsenic and mercury in rural aqueducts in the periphery of the city. These elements are important chemical parameters to monitor the quality of drinking water in the water supply network of the city. We analyzed 319 samples during the last half of 2010 and the first half of 2011 as a requirement of the Regulation 1575 of 2007. In the 99.38% of the samples, arsenic was no detectable, and the remaining 0.62% was below the reference value (0.01 mg L-1). Regarding to mercury, out of 319 water samples that were analyzed, in 274 (85%) the element was not detectable, and in 45 (15%) results were close to the reference value. In conclusion, the water samples analyzed for mercury and arsenic in 2010 and 2011 yielded no detectable levels that may affect the health of the population in Bogotá. © 2014 Taylor & Francis Group.
Argudin M.A.,University of Oviedo |
Mendoza M.C.,University of Oviedo |
Gonzalez-Hevia M.A.,Laboratorio Of Salud Publica |
Bances M.,Laboratorio Of Salud Publica |
And 2 more authors.
Applied and Environmental Microbiology | Year: 2012
Staphylococcal food poisoning, one of the most common food-borne diseases, results from ingestion of one or more staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus in foods. In the present study, 64 S. aureus isolates recovered from foods and food handlers, associated or not associated with food-poisoning outbreaks in Spain, were investigated. They were assigned to 31 strains by spa typing, multilocus sequence typing (MLST), exotoxin gene content, and antimicrobial resistance. The strains belonged to 10 clonal complexes (CCs): CC5 (29.0%), CC30 (25.8%), CC45 (16.1%), CC8, CC15 (two strains each), CC1, CC22, CC25, CC59, and CC121 (one strain each). They contained hemolysin genes (90.3%); lukED (77.4%); exfoliatin genes eta, etd (6.5% each), and etb (3.2%); tst (25.8%); and the following enterotoxin or enterotoxin-like genes or clusters: sea (38.7%), seb (12.9%), sec (16.1%), sed-selj with or without ser (22.9%), selk-selq (6.5%), seh, sell, selp (9.7% each), egc1 (32.3%), and egc2 (48.4%). The number of se and sel genes ranged from zero to 12. All isolates carrying tst, and most isolates with genes encoding classical enterotoxins (SEA, SEB, SEC, and SED), expressed the corresponding toxin(s). Two CC5 isolates from hamburgers (spa type t002, sequence type 5 [ST5]; spa type t2173, ST5) were methicillin resistant and harbored staphylococcal cassette chromosome mec (SCCmec) IVd. Six (19.4%) were mupirocin resistant, and one (spa type t120, ST15) from a food handler carried mupA (MIC, 1,250 μg/ml). Resistance to ampicillin (blaZ) (61.3%), erythromycin (ermA-ermC or ermC) (25.8%), clindamycin (msrAmsrB or msrB) (16.1%), tetracycline (tetK) (3.2%), and amikacin-gentamicin-kanamycin-tobramycin (aphA with aacA plus aphD or aadD) (6.5%) was also observed. The presence of S. aureus strains with an important repertoire of virulence and resistance determinants in the food chain represents a potential health hazard for consumers and merits further observation. © 2012, American Society for Microbiology.
Garcia P.,University of Oviedo |
Guerra B.,Federal Institute for Risk Assessment BfR |
Bances M.,Laboratorio Of Salud Publica |
Mendoza M.C.,University of Oviedo |
Rodicio M.R.,University of Oviedo
Journal of Antimicrobial Chemotherapy | Year: 2011
Objectives: To broaden knowledge of the molecular bases and genetics of multidrug resistance in clinical isolates of Salmonella enterica serotype 4,5,12:i:- belonging to the Spanish clone. Methods: The relatedness of the isolates was determined by phage typing and XbaI-PFGE. Resistance genes, integrons and transposable elements were identified by PCR amplification and sequencing. Plasmids were characterized by alkaline lysis, S1-PFGE, conjugation, replicon typing and Southern blot hybridization. Results: The isolates were closely related and resistant to five to seven antimicrobials (ampicillin, chloramphenicol, gentamicin, streptomycin/spectinomycin, sulphonamides, trimethoprim and tetracycline, arranged in different combinations). Most of the responsible genes were provided by a conventional class 1 integron with the dfrA12-orfF-aadA2 variable region, an atypical class 1 integron containing sul3 next to the estX-pspaadA2-cmlA1-aadA1 variable region and a truncated Tn1721 transposon carrying tet(A). A defective Tn21 with the mer operon and ISVsa3 associated with sul2 were also detected. All resistance genes and mobile genetic elements were located on large, non-conjugative and highly variable plasmids carrying one (A/C) or two (A/C and N) replicons, as well as virulence genes of pSLT. Conclusions: IncA/C plasmids are responsible for multidrug resistance in an increasing number of relevant human and animal bacterial pathogens, and hence are regarded as an important threat to public health. Those found in the Spanish clone of Salmonella 4,5,12:i:- constitute a relevant example of short-term evolution, and could have been involved in the successful adaptation of this pathogen. © The Author 2010. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
PubMed | Laboratorio Of Salud Publica
Type: Journal Article | Journal: Acta cytologica | Year: 2016
To estimate the frequency of human papillomavirus (HPV) infection and the genotype distribution of HPV among women with a Pap smear showing atypical squamous cells of undetermined significance (ASC-US) attending the Program for the Detection and Control of Cervical Cancer in Bogot, Colombia.Cervical samples from 200 women with an ASC-US Pap smear were analyzed for the presence of HPV DNA and genotype distribution using a commercial molecular technique (Linear Array; Roche Molecular Systems, USA).HPV infection was found in 140 women (70%). High-risk HPV types were present in 46.4% of the samples; 16.4% showed a low-risk HPV type, and 37.1% showed both. Of the positive samples, 42.9% were infected with a single viral genotype, whereas 57.1% exhibited multiple HPV infections. The most common HPV genotypes were HPV 16, 53, and 52 with a prevalence of 26.4, 16.4, and 13.6%, respectively.The epidemiological characterization of HPV infections described in this study might guide actions for epidemiological surveillance to strengthen the program in Bogot and to develop appropriate HPV vaccination programs.