Laboratorio Of Recursos Geneticos

Fortaleza, Brazil

Laboratorio Of Recursos Geneticos

Fortaleza, Brazil
SEARCH FILTERS
Time filter
Source Type

dos Santos J.M.,Federal University of Alagoas | Barbosa G.V.S.,Federal University of Alagoas | Neto C.E.R.,Laboratorio Of Genomica E Proteomica | Almeida C.,Laboratorio Of Recursos Geneticos
Crop Breeding and Applied Biotechnology | Year: 2014

Sugarcane has hermaphrodite flowers, however, selfing and cross pollination may occur, resulting in selfed or hybrid progeny. The aim of this study was to analyze the paternity of progenies from biparental crosses, in order to identify true hybrids or progenies originating from pollen of unknown origin. Seventy-six progenies from four crosses were analyzed using three highly polymorphic microsatellite markers (SSR). Progenies showed moderate genetic similarity and were grouped into four distinct groups, according to the crosses. Transmission of alleles from parents to offspring was clearly observed, in which selfed individuals were not observed, and only true hybrids or progeny resulting from fertilization with pollen uncommon to both parents were. Results showed that there was contamination with pollen from unknown parents in sugarcane crosses, suggesting that errors in the pedigree may occur, and adjustment in the crossing procedure would decrease progenies from pollen of unknown origin.


Pessoa A.C.B.P.,Laboratorio Of Recursos Geneticos | De Castro A.C.R.,Laboratorio Of Recursos Geneticos | Gallao M.I.,Federal University of Ceará
Acta Horticulturae | Year: 2013

Anthurium bonplandii and A. plowmanii belong to the Araceae family, which have high potential for use as ornamental plants. They are native to Brazil, and their cultivation and sale may represent an alternative source of novelties to the national floriculture. The aim of this work was to understand the leaf anatomy of these plants. Fully expanded leaves of plants selected from the Embrapa Germplasm Bank of Ornamental Tropical Plants located in Fortaleza-CE were used. The leaves were subjected to sodium hypochlorite for analysis of the epidermis in paradermic order. For the cross section analysis, the leaves were fixed in FAA50 (formaldehyde, acetic acid and alcohol), dehydrated in ethanol, subjected to pre-infiltration solution, infiltration solution and inserted in Leica historesin. The material was cut into semiautomatic microtome with a thickness of 10μm and the sections were stained with Toluidine Blue for structural analysis. It was also made free hand cuts to materials fixed for histochemical analysis. Phloroglucinol acid was used for detection of lignin and phenolic compounds, Sudan III was used for detection of lipids, and Lugol was used for starch detection. The two species had similar anatomical features. Both have a uniseriate epidermis with paracytic stomata in abaxial face and are completely covered by a cuticle. The mesophyll consists of chlorenchyma. In A. bonplandii the vascular bundles are of the collateral type, completely surrounded by sclerenchyma. In the midrib and petiole, they are surrounded by parenchyma cells rich in starch. Idioblasts containing phenolic compounds and calcium oxalate crystals are also found. In A. plowmanii the parenchyma cells have fewer starch grains than A. bonplandii and there are no phenolic compounds idioblasts dispersed in the ground parenchyma. These features may help understand many aspects of this leaves, such as external appearance and adaptation to environmental conditions.


Pessoa A.C.B.P.,Laboratorio Of Recursos Geneticos | De Castro A.C.R.,Laboratorio Of Recursos Geneticos | Sousa D.J.L.,Federal University of Ceará | Gallao M.I.,Federal University of Ceará
Acta Horticulturae | Year: 2013

Anthurium affine Schott and Anthurium raimundii Mayo, Haigh & Nadruz are two native species of northeast Brazil and have high potential for use as ornamental plants. They belonging to the Araceae family and can survive in stressful environmental conditions. The aim of this work was to describe the anatomy of these plants and possible correlation to environmental aspects which may be important factors in the leaf durability. Fully expanded leaves of plants selected from the Embrapa Germplasm Bank of Ornamental Tropical Plants located in Fortaleza-CE were fixed in FAA, dehydrated in ethanol, subjected to pre-infiltration solution, infiltration solution and inserted in Leica Historesin. The material was cut into semiautomatic microtome with a thickness of 10 μm and the sections were stained with Toluidine Blue for structural analysis. For histochemical analysis, free hand cuts were obtained from the fixed material. Phloroglucinol acid was used for detection of lignin and phenolic compounds, Sudan III was used for detection of lipids, and for the detection of starch Lugol was used. The leaves were subjected to sodium hypochlorite for analysis of the epidermis in paradermic order. The two species had similar anatomical features. Both have a uniseriate epidermis with paracytic stomata in abaxial face and are completely covered by a thick cuticle. The mesophyll consists of chlorenchyma. In A. affine and A. raimundii the vascular bundles are of the collateral type, completely surrounded by fibers, which are concentrated more strongly in front of the phloem. The parenchyma of the midrib and petiole are rich in starch. Many idioblasts were observed, containing phenolic compounds and calcium oxalate crystals. The thick cuticle may prevent excessive water loss by evapotranspiration and the large amount of stored energy reserves can help the plant to survive periods of dryness, typical of northeastern Brazil.

Loading Laboratorio Of Recursos Geneticos collaborators
Loading Laboratorio Of Recursos Geneticos collaborators