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Fonseca L.,University of Coimbra | Cardoso J.M.S.,University of Coimbra | Lopes A.,University of Coimbra | Pestana M.,Laboratorio Of Qualidade Agricola | And 4 more authors.
Helminthologia | Year: 2012

Summary: The environmental conditions in Madeira Island are favorable for the presence and dissemination of the pinewood nematode (PWN), Bursaphelenchus xylophilus. Five hundred Pinus pinaster wood samples were collected in several forest areas and PWN was detected in 22. 8 % of the samples. Bursaphelenchus xylophilus isolates from Madeira Island displayed the species-specific diagnostic characters. A morphological variation in the female tail terminus was detected. In most females, the tail presented a broadly rounded terminus and, occasionally, a digitate terminus with a terminal nipple-like extension resembling a mucro. PCR ITS-RFLP analysis revealed that Madeira Island isolates exhibited patterns specific to the species B. xylophilus and similar to virulent isolates. Amplified ITS regions were further sequenced and no genetic diversity was found for this genomic region among 17 Portuguese isolates (Madeira Island and Continental Portugal). Phylogenetic analysis revealed that Portuguese isolates grouped with isolates from China, Korea and one isolate from Japan. © 2012 Versita Warsaw and Springer-Verlag Wien.


Rota J.,University of Turku | Aguiar A.M.F.,Laboratorio Of Qualidade Agricola | Karsholt O.,Copenhagen University
Nota Lepidopterologica | Year: 2014

We review and illustrate the four species of Choreutidae recorded from Madeira - Anthophila threnodes (Walsingham), A. fabriciana (Linnaeus), Choreutis nemorana (Hubner), and Tebenna micalis (Mann) - and describe and illustrate for the first time the male of A. threnodes, as well as the biology of this Madeiran endemic. We provide brief notes on each of the species and give short diagnoses for correctly identifying them. Finally, we discuss previous misidentifications of Madeiran choreutids and the occurrence of choreutids on other oceanic islands.


Augustinos A.A.,University of Ioannina | Augustinos A.A.,University of Western Greece | Santos-Garcia D.,University of Valencia | Dionyssopoulou E.,University of Ioannina | And 17 more authors.
PLoS ONE | Year: 2011

Aphids are a serious threat to agriculture, despite being a rather small group of insects. The about 4,000 species worldwide engage in highly interesting and complex relationships with their microbial fauna. One of the key symbionts in arthropods is Wolbachia, an α-Proteobacterium implicated in many important biological processes and believed to be a potential tool for biological control. Aphids were thought not to harbour Wolbachia; however, current data suggest that its presence in aphids has been missed, probably due to the low titre of the infection and/or to the high divergence of the Wolbachia strains of aphids. The goal of the present study is to map the Wolbachia infection status of natural aphids populations, along with the characterization of the detected Wolbachia strains. Out of 425 samples from Spain, Portugal, Greece, Israel and Iran, 37 were found to be infected. Our results, based mainly on 16S rRNA gene sequencing, indicate the presence of two new Wolbachia supergroups prevailing in aphids, along with some strains belonging either to supergroup B or to supergroup A. © 2011 Augustinos et al.


Aguiar A.M.,Laboratorio Of Qualidade Agricola | Pombo D.A.,University of Porto | Goncalves Y.M.,Museu de Historia Natural do Funchal
Journal of insect science (Online) | Year: 2014

Two species of stick insects are currently known to be present in the Macaronesian archipelagos: Clonopsis gallica (Charpentier) (Phasmatodea: Bacillidae) on the Canary Islands and in the Azores and Carausius morosus (Sinéty) (Phasmatidae) in the Azores. Here, we provide the first reliable records of the presence and distribution of C. gallica and C. morosus on Madeira Island. Egg and adult stages are briefly described along with some notes on the life history of these species in captivity. Data on islandwide distribution are based on specimens donated by the public in response to an article published in a daily newspaper. This method of data collection raised great popular interest in stick insects. The role of newspapers as a means of communicating awareness in biodiversity issues is discussed. This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.


Aguiar A.M.F.,Laboratorio Of Qualidade Agricola | Pombo D.A.,University of Madeira | Pombo D.A.,University of Porto | Goncalves Y.M.,Museu de Historia Natural Do Funchal
Journal of Insect Science | Year: 2014

Two species of stick insects are currently known to be present in the Macaronesian archipelagos: Clonopsis gallica (Charpentier) (Phasmatodea: Bacillidae) on the Canary Islands and in the Azores and Carausius morosus (Sinéty) (Phasmatidae) in the Azores. Here, we provide the first reliable records of the presence and distribution of C. gallica and C. morosus on Madeira Island. Egg and adult stages are briefly described along with some notes on the life history of these species in captivity. Data on island-wide distribution are based on specimens donated by the public in response to an article published in a daily newspaper. This method of data collection raised great popular interest in stick insects. The role of newspapers as a means of communicating awareness in biodiversity issues is discussed. © This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.


PubMed | University of Porto, Museu de Historia Natural do Funchal and Laboratorio Of Qualidade Agricola
Type: | Journal: Journal of insect science (Online) | Year: 2014

Two species of stick insects are currently known to be present in the Macaronesian archipelagos: Clonopsis gallica (Charpentier) (Phasmatodea: Bacillidae) on the Canary Islands and in the Azores and Carausius morosus (Sinty) (Phasmatidae) in the Azores. Here, we provide the first reliable records of the presence and distribution of C. gallica and C. morosus on Madeira Island. Egg and adult stages are briefly described along with some notes on the life history of these species in captivity. Data on islandwide distribution are based on specimens donated by the public in response to an article published in a daily newspaper. This method of data collection raised great popular interest in stick insects. The role of newspapers as a means of communicating awareness in biodiversity issues is discussed.


Tennent W.J.,Natural History Museum in London | Aguiar A.M.F.,Laboratorio Of Qualidade Agricola | Healey D.,Bracklinn | Ferreira A.,Rua da Escola
Entomologist's Gazette | Year: 2013

Occurrence of the migrant nymphalid butterfly Hypolimnas misippus (Linnaeus, 1764), on the islands of Madeira and Porto Santo (Portugal) in November 2012 is reported and discussed. Historical records of H. misippus on the islands of Macaronesia (Azores, Canary Islands, Madeira, Cape Verde Islands) are briefly examined; the appearance of the migrant pierid butterfly Catopsilia florella Fabricius, 1775, and its apparent failure to persist on Madeira between 1999 and 2003 is also considered. Anecdotal evidence regarding the reason occasional small-scale migrations may fail to persist is briefly explored.

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