Laboratorio Of Parasitoides

Londrina, Brazil

Laboratorio Of Parasitoides

Londrina, Brazil
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Queiroz A.P.,Instituto Agronomico do Parana IAPAR | de Freitas Bueno A.,Laboratorio Of Parasitoides | Pomari-Fernandes A.,Federal University of Paraná | Grande M.L.M.,State University Londrina | And 2 more authors.
Bulletin of Entomological Research | Year: 2017

Egg parasitoid rearing on factitious hosts is an important step to reduce costs and increase availability of the biocontrol agent but it requires quality control to achieve success in field conditions. To this end, this study evaluated the quality of Telenomus remus (Hymenoptera: Platygastridae) reared on Corcyra cephalonica (Lepidoptera: Pyralidae) for until 45 generations. In the first bioassay, we evaluated the body size of the laboratory-produced parasitoids. In the second bioassay, flight activity was examined, measuring the percentage of ‘flyers’, ‘walkers’ and ‘deformed’ parasitoids. The third bioassay assessed parasitism on Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs. Our data indicate that the laboratory-reared parasitoid neither lost its ability to fly nor to parasitize S. frugiperda eggs. In conclusion, quality did not decrease significantly during 45 generations, and therefore rearing of T. remus on C. cephalonica as factitious host promises to be successful. Copyright © Cambridge University Press 2017


Silva G.V.,Federal University of Paraná | de Freitas Bueno A.,Laboratorio Of Parasitoides | Bortolotto O.C.,Federal University of Paraná | dos Santos A.C.,Dow AgroSciences | Pomari-Fernandes A.,University Federal da Fronteira Sul
Revista Brasileira de Entomologia | Year: 2016

This study aimed to evaluate the development and reproduction of the black armyworm, Spodoptera cosmioides when larvae fed on leaves of Bt-corn hybrids, expressing a single Cry1F and also Cry1F, Cry1A.105 and Cry2Ab2 in pyramided corn and their non-Bt-isoline (hybrid 2B688), as well as on leaves of two soybean isolines expressing the Cry1Ac protein and its non-Bt isoline (A5547-227). We also assessed the effect of these Bt and non-Bt plants on the leaf consumption rate of S. cosmioides larvae. This pest was unable to develop when fed on any of the corn isolines (Bt and non-Bt). When both 1st and 3rd instar larvae were fed on corn leaf, mortality was 100% in both Bt and non-Bt corn. In contrast, when corn leaves were offered to 5th instar larvae, there were survivors. Defoliation and leaf consumption was higher with non-Bt corn than with both of the Bt corn isolines. There was no negative effect of Bt soybean leaves on the development and reproduction of S. cosmioides with respect to all evaluated parameters. Our study indicates that both Bt and non-Bt corn adversely affect the development of S. cosmioides while Bt soybean did not affect its biology, suggesting that this lepidopteran has major potential to become an important pest in Bt soybean crops. © 2016 Sociedade Brasileira de Entomologia


Silva G.V.,Federal University of Paraná | Pasini A.,State University Londrina | de Freitas Bueno A.,Laboratorio Of Parasitoides | Bortolotto O.C.,Federal University of Paraná | And 2 more authors.
Revista Brasileira de Entomologia | Year: 2014

No impact of Bt soybean that express Cry1Ac protein on biological traits of Euschistus heros (Hemiptera, Pentatomidae) and its egg parasitoid Telenomus podisi (Hymenoptera, Platygastridae). Biological traits of the stink bug Euschistus heros and its main biological control agent Telenomus podisi were evaluated under controlled environmental conditions (25 ± 2°C; 60 ± 10% RH; and 14/10 h photoperiod) by placing first instar nymphs into Petri dishes with pods originating from two soybean isolines (Bt-soybean MON 87701 × MON 89788, which expresses the Cry1Ac protein, and its near non-Bt isoline A5547) where they remained until the adult stage. Due to gregarious behavior exhibited by first instar nymphs, they were individualized only when at the second instar. Adults were separated by sex and weighed, and pronotum width of each individual was subsequently measured. They were placed into plastic boxes containing soybean grains of the same soybean isoline as food source. Egg viability and female fecundity were assessed in adult individuals. Adult females of T. podisi (up to 24h old) were placed with eggs of E. heros from mothers reared on both soybean isolines. Nymphal development time, insect weight, pronotum width, sex ratio, female fecundity, and egg viability (% emergence) of Euschistus heros did not differ between treatments. Eggto- adult development time, female longevity, sex ratio, and percentage of parasitized eggs were not impacted by the Bt-soybean (expressing Cry1Ac protein). Results indicate that the Bt-soybean, MON 87701 × MON 89788, has no direct significant impact on the two studied species.


Bortolotto O.C.,Federal University of Paraná | Bortolotto O.C.,Laboratorio Of Parasitoides | Silva G.V.,Federal University of Paraná | Silva G.V.,Laboratorio Of Parasitoides | And 7 more authors.
Bulletin of Entomological Research | Year: 2014

Genetically modified crops with insect resistance genes from Bacillus thuringiensis Berliner (Bt-plants) are increasingly being cultivated worldwide. Therefore, it is critical to improve our knowledge of their direct or indirect impact not only on target pests but also on non-target arthropods. Hence, this study evaluates comparative leaf consumption and performance of Spodoptera eridania (Cramer), a species that is tolerant of the Cry1Ac protein, fed with Bt soybean, MON 87701×MON 89788 or its non-Bt isoline. We also assessed the comparative performance of the egg parasitoid Telenomus remus Nixon on eggs of S. eridania produced from individuals that fed on these two soybean isolines as larvae. Results showed that Bt soybean reduced by 2 days larval development and increased by 3 days adult male longevity. Therefore, we conclude that the effect of Bt soybean MON 87701×MON 89788 on S. eridania development and reproduction is small, and favorable to pest development. These differences are less likely to directly result from the toxin presence but indirectly from unintended changes in plant characteristics caused by the insertion of the transgene. Our results should be viewed as an alert that S. eridania populations may increase in Bt soybeans, but on the other hand, no adverse effects of this technology were observed for the egg parasitoid T. remus which can help to prevent S. eridania outbreaks on these crops. © Cambridge University Press 2014.


PubMed | Federal University of Paraná, Laboratorio Of Parasitoides and Monsanto Corporation
Type: Journal Article | Journal: Bulletin of entomological research | Year: 2014

Genetically modified crops with insect resistance genes from Bacillus thuringiensis Berliner (Bt-plants) are increasingly being cultivated worldwide. Therefore, it is critical to improve our knowledge of their direct or indirect impact not only on target pests but also on non-target arthropods. Hence, this study evaluates comparative leaf consumption and performance of Spodoptera eridania (Cramer), a species that is tolerant of the Cry1Ac protein, fed with Bt soybean, MON 87701MON 89788 or its near [corrected] non-Bt isoline. Using this species as a model, we assessed [corrected] the comparative performance of the egg parasitoid Telenomus remus Nixon on eggs of S. eridania produced from individuals that fed on these two soybean genotypes [corrected] as larvae. Results showed that Bt soybean did not affect pest foliage consumption, but did reduce larvel duration by two days despite larvae in both treatments having six instars. Nevertheless, survival of S. eridania larvae, pupal weight, sex ratio, fecundity and longevity of female moths, and egg viability did not differ between Bt and non-Bt soybeans. Adult longevity of S. eridania males was increased when caterpillars were fed with Bt soybean versus the near isoline. No adverse effects of this technology were observed for the egg parasitoid T. remus. [corrected].

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