Laboratorio Of Oncologia Genomica

Unidad San Miguel Jagüeyes, Mexico

Laboratorio Of Oncologia Genomica

Unidad San Miguel Jagüeyes, Mexico
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Gonzalez-Herrera A.L.,Laboratorio Of Oncologia Molecular | Salgado-Bernabe M.,Laboratorio Of Oncologia Molecular | Velazquez-Velazquez C.K.,Laboratorio Of Oncologia Molecular | Salcedo-Vargas M.,Laboratorio Of Oncologia Genomica | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2015

Background: Cervical cancer (CeCa) is the second most common cancer in women in developing countries, and human papilloma virus (HPV) is the primary etiological factor. Aberrant expression of HOX transcription factors has been observed in several types of cancer. To date, however, no reports exist on the expression of HOXB2 and HOXB13 proteins during neoplastic progression in CeCa and its correlation with HPV infection. Materials and Methods: Expression of HOXB2 and HOXB13 proteins was assessed in tissue microarrays from normal cervical epithelium, cervical intraepithelial neoplasias grade 1-3, and CeCa. HPV was detected by PCR and sequencing. Expression of HOX-positive cells was determined in each diagnostic group. Results: Percentage of HOXB2- and HOXB13-positive cells gradually increased from means of 10.9% and 16.7%, respectively, in samples from healthy women, to 75.2% and 88.6% in those from CeCa patients. Frequency of HPV infection also increased from 13% in healthy tissue samples to 92.3% in CeCa. Both HOXB2 and HOXB13 proteins were preferentially expressed in HPV+ samples. Conclusions: The present study represents the first report on the expression of both HOXB2 and HOXB13 proteins through cervix tumorigenesis, providing evidence that increased expression of such proteins is a common event during progression to CeCa.

Pacheco-Rivera R.A.,Laboratorio Of Diagnostico Molecular | Pacheco-Rivera R.A.,Laboratorio Of Oncologia Genomica | Hernandez-Zamora E.,Servicio de Genetica | Gonzalez-Yebra B.,University of Guanajuato | And 5 more authors.
Clinical and Experimental Medicine | Year: 2011

The most important mutation associated with Multiple Endocrine Neoplasia type 2B (MEN 2B) is the change of thymine to cytosine in codon 918 of exon 16 in the RET oncogene (ATG → ACG). The aim of this work was to develop a single oligoarray by using tandem hybridization to detect the T918C/RET mutation for MEN 2B patients. Two genetically non-related families were studied; each family had a member affected by MEN2B. Both patients presented the T918C/RET mutation in a heterozygous fashion. None of the relatives was positive for this mutation; thus, these cases arose de novo. The proper mutation was confirmed by with different tools, PCR-Fok I endonuclease, direct sequencing, and also using our oligoarray. In this case, it is suitable to use a DNA target smaller than 150 bases with single- or doublestranded DNA and short probes of 7-mer. It was also possible to detect the mutation by employing different sources of DNA, fresh or paraffin-embedded tissues. Therefore, the present oligoarray can identify the most common M918T mutation of RET oncogene from a variety of DNA sources with good specificity and be a good alternative in the molecular diagnosis for MEN 2B cases. © Springer-Verlag 2011.

Peralta-Rodriguez R.,Laboratorio Of Oncologia Genomica | Romero-Morelos P.,Laboratorio Of Oncologia Genomica | Villegas-Ruiz V.,Laboratorio Of Oncologia Genomica | Mendoza-Rodriguez M.,Laboratorio Of Oncologia Genomica | And 4 more authors.
Infectious Agents and Cancer | Year: 2012

Background: Human Papillomavirus (HPV) in cervical epithelium has been identified as the main etiological factor in the developing of Cervical Cancer (CC), which has recently become a public health problem in Mexico. This finding has allowed for the development of vaccines that help prevent this infection. In the present study, we aimed to determine the prevalence and HPV type-distribution in Mexican women with CC, high-grade squamous intraepithelial lesion (HSIL), low-grade squamous intraepithelial lesion (LSIL), and Normal cytology (N) to estimate the impact of the HPV vaccines. Methods. The PubMed database was used to identify and review all articles that reported data on HPV prevalence in CC, precursor lesions, and normal cytology of Mexican women. Results: A total of 8,706 samples of the tissues of Mexican women were stratified according to diagnosis as follows: 499 for CC; 364 for HSIL; 1,425 for LSIL, and 6,418 for N. According to the results, the most prevalent genotypes are the following: HPV16 (63.1%), -18 (8.6%), -58, and -31 (5%) for CC; HPV-16 (28.3%), 58 (12.6%), 18 (7.4%), and 33 (6.5%) for HSIL; HPV-16 (13.1%), 33 (7.4%), 18 (4.2%), and 58 (2.6%) for LSIL, and HPV-16 (3.4%), 33 (2.1%), 18, and 58 (1.2%) for N. Conclusions: Taken together, genotypes 58 and 31 (10%) are more common than type 18 (8.6%) in CC. Therefore, the inclusion of these two genotypes in a second-generation vaccine would provide optimal prevention of CC in Mexico. © 2012 Peralta-Rodríguez et al.

Herrera-Romano L.,Instituto Mexicano del Seguro Social IMSS | Herrera-Romano L.,University Puebla | Herrera-Romano L.,National Polytechnic Institute of Mexico | Fernandez-Tamayo N.,Hospital Institute Seguridad Y Servicios Sociales Of Los Trabajadores Al Servicio Of Los Poderes | And 9 more authors.
Medical Oncology | Year: 2012

The role of human papillomavirus (HPV) in breast cancer is controversial. We evaluated 118 breast carcinomas and two paraffin-embedded tissues of lesions of the nipple of Mexican patients for HPV sequences. No carcinoma sample exhibited koilocytosis, in contrast to lesions of the nipple. We subjected purified DNAs to PCR employing two HPV16/E6 or GP5/6 primer set oligonucleotides. Results showed that HPV DNA sequences were absent in the breast tissues. Absence of HPV in breast carcinoma failed to support an association between HPV infection and this carcinoma. © Springer Science+Business Media, LLC 2011.

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