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Schweinberger B.M.,Laboratorio Of Neuroprotecao E Doencas Metabolicas | Schweinberger B.M.,Federal University of Rio Grande do Sul | Schwieder L.,Laboratorio Of Neuroprotecao E Doencas Metabolicas | Scherer E.,Laboratorio Of Neuroprotecao E Doencas Metabolicas | And 5 more authors.
Metabolic Brain Disease | Year: 2014

In the present study we developed a chemically induced experimental model for gestational hypermethioninemia in rats and evaluated in the offspring the activities of Na+,K+-ATPase and Mg2+-ATPase, as well as oxidative stress parameters, namely sulfhydryl content, thiobarbituric acid-reactive substances and the antioxidant enzymes superoxide dismutase and catalase in encephalon. Serum and encephalon levels of methionine and total homocysteine were also evaluated in mother rats and in the offspring. Pregnant Wistar rats received two daily subcutaneous injections of methionine throughout the gestational period (21 days). During the treatment, a group of pregnant rats received dose 1 (1.34 μmol methionine/g body weight) and the other one received dose 2 (2.68 μmol methionine/g body weight). Control group received saline. After the rats give birth, a first group of pups was killed at the 7th day of life and the second group at the 21th day of life for removal of serum and encephalon. Mother rats were killed at the 21th day postpartum for removal of serum and encephalon. Both doses 1 and 2 increased methionine levels in encephalon of the mother rats and dose 2 increased methionine levels in encephalon of the offspring. Maternal hypermethioninemia also decreased the activities of Na+,K+-ATPase, Mg2+-ATPase and catalase, as well as reduced total sulfhydryl content in the encephalon of the pups. This chemical model seems to be appropriate for studies aiming to investigate the effect of maternal hypermethioninemia on the developing brain during gestation in order to clarify possible neurochemical changes in the offspring. © 2013 Springer Science+Business Media.


Tagliari B.,Laboratorio Of Neuroprotecao E Doencas Metabolicas | Tagliari B.,Laboratorio Of Erros Inatos Do Metabolismo | Noschang C.G.,Federal University of Rio Grande do Sul | Ferreira A.G.K.,Laboratorio Of Neuroprotecao E Doencas Metabolicas | And 9 more authors.
Metabolic Brain Disease | Year: 2010

Since chronic stress has been used widely for studying clinical depression and that brain energy metabolism and oxidative stress might be involved in the pathophysiology of this illness, the objective of this study was investigate the activities of pyruvate kinase, complex II and IV (cytocrome c oxidase) in hippocampus and prefrontal cortex of rats submitted to chronic variable stress. We also evaluated if vitamins E and C administration could prevent such effects. During 40 days adult rats from the stressed group were subjected to one stressor per day, at a different time each day, in order to minimize predictability. The stressed group had gained less weight while its immobilization time in the forced swimming test was greater than that of the control group. Results showed that stressed group presented an inhibition in the activities of complex II and cytochrome c oxidase in prefrontal cortex, while in hippocampus just complex IV was inhibited. Pyruvate kinase activity was not altered in stressed group when compared to control. Vitamins E and C administration prevented the alterations on respiratory chain caused by stress. These data suggest that the impairment of energy metabolism and oxidative stress could be related with the pathogenic pathways in stress related disorders. © 2010 Springer Science+Business Media, LLC.

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