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Mexico City, Mexico

Santos R.C.V.,Centro Universitario Franciscano | Santos R.C.V.,Laboratorio Of Nanotecnologia | Lopes L.Q.S.,Centro Universitario Franciscano | Lopes L.Q.S.,Laboratorio Of Nanotecnologia | And 14 more authors.
Journal of Asia-Pacific Entomology | Year: 2014

Paenibacillus larvae and Melissococcus plutonius are the primary bacterial pathogens of honeybees and the causative agents of American and European foulbrood disease (AFB and EFB) respectively. Such diseases have been gaining importance since there are few therapeutic options beyond the reporting of microorganisms resistant to conventional antibiotics. Due to the inefficiency and/or low efficacy of some antibiotics, researches with nanotechnology represent, possibly, new therapeutic strategies. Nanostructured drugs have presented some advantages over the conventional medicines, such as slow, gradual and controlled release, increased bioavailability, and reduced side-effects, among others. In this study, in vitro antimicrobial activity of tea tree oil (TTO) nanoparticles against Paenibacillus species, including P. larvae and M. plutonius strains was evaluated. Minimal inhibitory concentration (MIC) in Mueller-Hinton or KSBHI broth by the microdilution method was assessed. TTO registered MIC values of 0.18-6.25%, while the MIC values obtained for the TTO nanoparticle were of 0.01-0.93%. The possible toxic effect of TTO and TTO nanoparticle has been assessed by the spraying application method in the concentrations higher than the MICs. Bee mortality was evident only in treatment with TTO and the TTO nanoparticles show no toxic effects after 7. days of observation. Our results showed for the first time that TTO nanoencapsulation presented a high activity against Paenibacillus species and M. plutonius strains showing that the use of nanotechnology may represent one alternative way for the treatment or prevention of AFB and EFB. •TTO nanoencapsulation presented a high activity against Paenibacillus species and M. plutonius strains showing that the use of nanotechnology may represent one alternative way for the treatment or prevention of AFB and EFB. © 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Source


Fabila D.A.,National Polytechnic Institute of Mexico | De La Rosa J.M.,National Polytechnic Institute of Mexico | Stolik S.,National Polytechnic Institute of Mexico | Moreno E.,National Polytechnic Institute of Mexico | And 7 more authors.
IEEE Latin America Transactions | Year: 2011

In this issue the development of a portable system for measuring fluorescence and reflectance signals of biological tissue is presented. It includes a mini-spectrometer HR4000CG-UV-NIR, with a working range from 200 to 1100 nm and a resolution of 0.5 nm. A light emitting diode at 365 nm with maximum radiant power of 230 mW was used as an excitation source. A bifurcated fiber optic with six 400 μm diameter irradiation fibers and one 400 μm collection fiber is used. The system is powered and controlled by a laptop, avoiding the use of an external power supply. A program to control the light source and to measure the spectra in real time was developed using LabVIEW. Preliminary results of ex vivo and in vivo fluorescence measurements of skin and brain tissues are presented and show that fluorescence and reflection could be used to diagnose and demarcate cancer boundaries. © 2005 IEEE. Source

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