Laboratorio Of Misure Ambientali E Tossicologiche

Pavia, Italy

Laboratorio Of Misure Ambientali E Tossicologiche

Pavia, Italy
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Sturchio E.,DIPIA Inc | Amadori A.,L.N. Age srl. | Businaro J.,Laboratorio Of Misure Ambientali E Tossicologiche | Ficociello B.,DIPIA Inc | And 3 more authors.
Giornale Italiano di Medicina del Lavoro ed Ergonomia | Year: 2012

Exposure to asbestos is the predominant cause of pleural mesothelioma (PM). The PM is a tumor difficult to diagnose, chemoresistant, and with rising Incidence. The long latency periods and the lack of preventive and therapeutic strategies for the MP, suggest that asbestos will be a social and health issue in the near future. Therefore, this overview focuses on current knowledge of epigenetic alterations and on the key role of microRNAs, small RNA s that negatively regulate gene expression, as biomarkers in PM development. Dysregulated microRNA expression pattern is specific for different cancers, including MP. MicroRNA expression analysis is a promising tool for diagnosis, typing of MP than normal tissue and other lung tumors and monitoring of new therapies. However, a better knowledge of miRNA signatures in PM is still necessary to verify the contribution of specific miRNAs as diagnostic biomarkers, also compared to different asbestos forms, exposure and subject work history. © PI-ME, Pavia 2012.


Turci R.,Laboratorio Of Misure Ambientali E Tossicologiche | Businaro J.,University of Pavia | Poma P.,University of Pavia | Minoia C.,Laboratorio Of Misure Ambientali E Tossicologiche
Giornale Italiano di Medicina del Lavoro ed Ergonomia | Year: 2012

Polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCs) are lipophilic and persistent compounds that were widely used in industrial and consumer products for decades until the late 70s. Due to their persistence and bioaccumulation, they are globally spread in the environment and may still be found in all environmental and biological media. Some have been classified as xenoestrogens or endocrine disruptors. Some others are known to have adverse effects for human health, including cancer. With the objective of establishing reference values, a fast and reliable method, previosuly developed and validated using single quadrupole mass spectrometry, was properly modified in order to simultaneously detect 10 organochlorine pesticides and 15 PCBs by using triple quadrupole. Use of triple quadrupole mass spectrometry has further increased the reliability of the method, so that it is now possible to collect more significant data concerning general population, which is the first step before the assessment of occupational exposure levels. © PI-ME, Pavia 2012.


Minoia C.,Laboratorio Of Misure Ambientali E Tossicologiche | Minoia C.,University of Pavia | Sturchio E.,1NAIL ex ISPESL | Porro B.,Laboratorio Of Misure Ambientali E Tossicologiche | And 3 more authors.
Giornale Italiano di Medicina del Lavoro ed Ergonomia | Year: 2011

Malignant mesothelioma is an aggressive cancer refractory to current therapies, the incidence of which is expected to rise in the next decades. Exposure to asbestos is a well known risk factor, as International Agency for Research and Cancer (IARC) classified this compound as group I (carcinogenic to humans). The lack of tumor biomarkers for diagnosis and medical survey plays a fundamental role for the development of a universally accepted therapeutic approach. In this review we evaluated the mechanism of asbestos carcinogenesis by analyzing activated oncogenes, genetic predisposition, and SV40 infection as cofactors. Therefore, interest has focused on microRNAs, 19-25 nucleotide-long single-stranded RNAs that negatively regulate gene expression by modulating translational efficiency of target genes involved in numerous cellular processes including development, differentiation, proliferation, apoptosis, and stress response. The analysis revealed a differential expression of miRNAs between mesothelioma and mesothelial cells, suggesting their potential role as oncogenes or tumor suppressor genes in mesothelioma oncogenesis. We have also investigated the role of polymorphism in the etiology and pathogenesis of mesothelioma, in order to evaluate the association between disease linked to asbestos exposure and genetic variability. The identification of dysregulated miRNAs or frequent genetic polymorphisms as potential diagnostic biomarkers or as prognostic factors for malignant mesothelioma could facilitate the surveillance procedure of subjects exposed to asbestos. © PI-ME, Pavia 2011.


PubMed | Laboratorio Of Misure Ambientali E Tossicologiche
Type: Journal Article | Journal: Giornale italiano di medicina del lavoro ed ergonomia | Year: 2012

Malignant mesothelioma is an aggressive cancer refractory to current therapies, the incidence of which is expected to rise in the next decades. Exposure to asbestos is a well known risk factor, as InternationalAgency for Research and Cancer (IARC) classified this compound as group I (carcinogenic to humans). The lack of tumor biomarkers for diagnosis and medical survey plays a fundamental role for the development of a universally accepted therapeutic approach. In this review we evaluated the mechanism of asbestos carcinogenesis by analyzing activated oncogenes, genetic predisposition, and SV40 infection as cofactors. Therefore, interest has focused on microRNAs, 19-25 nucleotide-long single-stranded RNAs that negatively regulate gene expression by modulating translational efficiency of target genes involved in numerous cellular processes including development, differentiation, proliferation, apoptosis, and stress response. The analysis revealed a differential expression of miRNAs between mesothelioma and mesothelial cells, suggesting their potential role as oncogenes or tumor suppressor genes in mesothelioma oncogenesis. We have also investigated the role of polymorphism in the etiology and pathogenesis of mesothelioma, in order to evaluate the association between disease linked to asbestos exposure andgenetic variability. The identification of dysregulated miRNAs or frequent genetic polymorphisms as potential diagnostic biomarkers or as prognostic factors for malignant mesothelioma could facilitate the surveillance procedure of subjects exposed to asbestos.

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