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De la Rosa-Ramos M.A.,Laboratorio Of Microbiologia Veterinaria | De la Rosa-Ramos M.A.,Laboratorio Of Bacteriologia Medica | Rodriguez-Cruz M.,Laboratorio Of Microbiologia Veterinaria | Lopez-Villegas E.O.,National Polytechnic Institute of Mexico | And 3 more authors.
Avian Pathology | Year: 2015

Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism. © 2015 Houghton Trust Ltd.

de Faria A.C.S.,Federal University of Mato Grosso | da Silva M.C.,Laboratorio Of Microbiologia Veterinaria | Filho J.X.O.,Federal University of Mato Grosso | de Oliveira J.T.,Federal University of Mato Grosso | And 4 more authors.
Ciencia Rural | Year: 2010

Streptococcus suis is a pathogen that affects the industrial production of swine worldwide. It is extremely important, because it is associated with pigs and humans diseases. The aim of this study was to determine the prevalence of Streptococcus suis type 2 in 201 samples of tonsils from clinically healthy animals by the PCR technique. The samples positive for S. suis type 2 were tested for the gene encoding extracellular factors (ef). The results showed that the prevalence (23.38%) was higher than other recent survey in the State, demonstrating that the PCR is a more sensitive method in relation to the bacterial isolation. There was a low occurrence of ef* gene in samples (1.49%) showing great importance to local swine population, because negative strains are potentially less virulent that positive strains.

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