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Laura S.,Marine Microbiology Laboratory Of The Expt Zooprophylactic Institute Of Piemonte Liguria E Valle Dao | Laura S.,Laboratorio Of Microbiologia Marina | Irene R.,Marine Microbiology Laboratory Of The Expt Zooprophylactic Institute Of Piemonte Liguria E Valle Dao | Roberta B.,Marine Microbiology Laboratory Of The Expt Zooprophylactic Institute Of Piemonte Liguria E Valle Dao | And 4 more authors.
Food and Environmental Virology | Year: 2012

In this study, we investigated the presence of enteric viruses such as norovirus (NoV), hepatitis A virus (HAV), hepatitis E virus (HEV), and adenovirus (HAdV), in vegetables available on the Italian markets. For this aim, 110 national and international "ready to eat" samples were collected and analyzed by biomolecular tests and positive samples were confirmed by sequencing. All samples (100 %) were negative for HAV, HEV, and HAdV, while 13.6 % (15/110) were positive for NoV. Actually there is not a formal surveillance system for NoV infections in Italy but we clearly demonstrated a potential risk associated with the consumption of "ready to eat" vegetables. This study confirmed for the first time in Italy the presence of norovirus in semi-dried tomatoes by PCR technique. © 2012 Springer Science + Business Media, LLC.


Serracca L.,Laboratorio Of Microbiologia Marina | Rossini I.,Laboratorio Of Microbiologia Marina | Battistini R.,Laboratorio Of Microbiologia Marina | Garrone A.,Laboratorio Microbiologia Molecolare e Analisi Genomiche | And 2 more authors.
Industrie Alimentari | Year: 2010

Regulation (EC) No 2073/2005 highlights the need to develop reliable detection methods for pathogenic Vibrio strains. To this purpose, we have evaluated the application of an integrated identification protocol by means ol biochemical and biomolecular methods to identity isolates from one hundred sea fish samples. Isolates previously positive to biochemical identification were subjected to species-specific PCR tor the identification of V. vulnificus, V. cholerae and V. parahaemolyticus toxigenic strains. The analyzed samples revealed the presence of 5 strains as V. vulnificus and 5 as V. cholerae, none of these were positive for V. parahaemolyticus. Sequencing confirmed only one sample as V. cholerae, allowing to correctly identify false positive samples. We can conclude that the biochemical tests should always be confirmed by molecular tests to obtain a correct identification.

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