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Santos S.N.,Laboratorio Of Microbiologia Ambiental Embrapa Meio Ambiente Cnpma | Ferraris F.K.,Laboratorio Of Quimica Of Produtos Naturais Farmanguinhos | De Souza A.O.,Instituto Butantan | Henriques M.D.G.,Laboratorio Of Quimica Of Produtos Naturais Farmanguinhos | And 2 more authors.
Symbiosis | Year: 2012

The recent increase in human diseases and cancers requires new drugs to combat them. Sources have been found in rare microorganisms, those from extreme habitats, and from endophytes. In this study, the biological activity of endophytic fungi associated with the Brazilian medicinal plant Combretum leprosum was assessed. Cytotoxic and antiproliferative effects were evaluated using seven human cancer cells lines (HeLa, ECV304, B16F10, J744, P388, Jurkat and k562). In addition the minimum inhibitory concentration (MIC) against pathogenic human fungal was determined using four Candida species and the filamentous fungi Cryptococcus neoformans and Trichophyton rubrum. A compound from extracts of phylotype Aspergillus oryzae CFE108 exhibited the most significant cytotoxicity effect against histiocytic sarcoma J774 (IC 50 of 0.80 μg mL-1), leukemia Jurkat (IC50 of 0.89 μg mL-1), bladder carcinoma ECV304 (IC50 of 3.08 μg mL-1) and cervical cancer HeLa (IC50 of 2.97 μg mL-1). The extract from phylotypes Fusarium oxysporum CFE177 displayed antifungal activity and inhibited the growth of Candida glabrata (4 μg mL-1) as well as that of C. neoformans and T. rubrum with the lowest MIC being 62.5 μg mL-1. In addition, the fractions from A. oryzae CFE108 showed marked morphological activity (rounding up) on endothelial cells (tEnd.1 cells), which is indicative of potential antivascular activity. Our results indicate that the endophytes associated with this medicinal plant may be a source of novel drugs. © 2012 Springer Science+Business Media Dordrecht. Source


Ferhan M.,University of Toronto | Ferhan M.,Laboratorio Of Microbiologia Ambiental Embrapa Meio Ambiente Cnpma | Santos S.N.,Laboratorio Of Microbiologia Ambiental Embrapa Meio Ambiente Cnpma | Melo I.S.,Laboratorio Of Microbiologia Ambiental Embrapa Meio Ambiente Cnpma | And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2013

Fungal species for ligninases production was investigated by 18S ribosomal DNA sequence analysis. Two primer sets were chosen to amplify a major part of the 18S rDNA, which resulted in intense PCR product of approximately 550-820 bp in size per sample. The results suggest that the 18S rDNA-based approach is a useful tool for identification of unknown potential fungal species for ligninases production. The isolated fungal species produces mainly manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related polyphenols. Time course studies showed that maximum production of ligninolytic enzymes MnP (64 IU L-1), lignin peroxidase (26.35 IU L-1), and laccase (5.44 IU L-1), respectively, were achieved after 10 days of cultivation under optimum conditions. Furthermore, the biological decolorization of Remazol Brilliant Blue R dye following 10 days of cultivation was 94 %. NCBI BLAST was used to search for closest matched sequences in the GenBank database and based on sequence homology the first BLAST hit was Dothioraceae sp. LM572 with accession number EF060858.1. © 2013 Springer Science+Business Media Dordrecht. Source

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