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Belo Horizonte, Brazil

Terra M.F.,Federal University of Lavras | Prado G.,Laboratorio Of Micotoxinas | Pereira G.E.,Embrapa Uva e Vinho Semiarido | Ematne H.J.,Federal University of Lavras | Batista L.R.,Federal University of Lavras
Journal of the Science of Food and Agriculture | Year: 2013

BACKGROUND: Ochratoxin A (OTA) is the main mycotoxin found in grapes, wines and grape juices and is considered one of the most harmful contaminants to human health. In this study, samples of tropical wines and grape juices from different grape varieties grown in Brazil were analysed for their OTA content by high-performance liquid chromatography. RESULTS: The detection and quantification limits for OTA were 0.01 and 0.03 μg L-1 respectively. OTA was detected in 13 (38.24%) of the samples analysed, with concentrations ranging from < 0.03 to 0.62 μg L-1. OTA was not detected in any of the grape juice samples. Most of the red wine samples proved to be contaminated with OTA (75%), while only one white wine sample was contaminated. However, the OTA levels detected in all samples were well below the maximum tolerable limit (2 μg L-1) in wine and grape juice established by the European Community and Brazilian legislature. CONCLUSION: The results of this study indicate a low risk of exposure to OTA by consumption of tropical wines and grape juices from Brazil. © 2012 Society of Chemical Industry. Source

Prado G.,Laboratorio Of Micotoxinas | Altoe A.F.,Programa de Iniciacao Cientifica Institucional | Gomes T.C.B.,Brazilian Nuclear Technology Development Center (CDTN) | Leal A.S.,Brazilian Nuclear Technology Development Center (CDTN) | And 8 more authors.
Brazilian Journal of Microbiology | Year: 2012

The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on extraction through acetone:water (85:15), immunoaffinity column purification followed by separation and detection in high efficiency liquid chromatography. Under the conditions of analysis, the Limits of Detection and Quantification were 0.6 μg kg-1 and 1.0 μg kg-1 respectively. The complete sets of analyses were carried out in duplicate. Aflatoxin B1 was noticed in a single sample (< 1.0 μg kg-1). The results revealed low aflatoxin B1 contamination in the products under analysis. However, it is required to establish a broad monitoring program in order to obtain additional data and check up on the actual extension of contamination. Source

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