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De La Cruz J.P.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | Del Rio S.,Service of Ophthalmology | Lopez-Villodres J.A.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | Villalobos M.A.,University of Malaga | And 2 more authors.
British Journal of Nutrition | Year: 2010

The aim of the present study is to evaluate the possible influence of virgin olive oil (VOO) on the effect of acetylsalicylic acid (ASA) in platelet aggregation, prostanoid and NO production and retinal vascular pattern in rats with experimental type 1-like diabetes. We used 100 male Wistar rats that were distributed into five groups: (1) non-diabetic rats (NDR); (2) untreated diabetic rats (DR); (3) DR treated with ASA (2mg/kg per d per os (p.o.);(5) DR treated with VOO (05ml/kg per d p.o.); (5) DR treated with ASA plus VOO. The duration of diabetes was 3 months, and each treatment was administered from the first day of diabetes. Variables that were quantified were platelet aggregation (Imax), thromboxane B2 (TxB2), aortic prostacyclin (6-keto-PGF1) and NO, and the percentage of retina with horseradish peroxidase-permeable vessels (HRP-PV). Diabetic rats showed a higher Imax (35%) and TxB2 (63%) than NDR, and a lower 6-keto-PGF11α, NO and HRP-PV than NDR (746%). ASA and VOO administration reduced these differences and prevented the percentage of HRP-PV (597% with ASA and 467% with VOO). The administration of ASA plus VOO showed a strong platelet inhibition (802 v. 234% for VOO and 506% for ASA+VOO, P<00001), and reduced HRP-PV differences to 316% (P<0001 with respect to DR and P<00001 with respect to DR treated with ASA). In conclusion, the administration of VOO to rats with type 1-like diabetes mellitus improves the pharmacodynamic profile of ASA, and increases its retinal anti-ischaemic effect. Copyright © The Authors 2010.


Cabrerizo S.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | De La Cruz J.P.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | Lopez-Villodres J.A.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | Munoz-Marin J.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | And 4 more authors.
Journal of Nutritional Biochemistry | Year: 2013

The aim of this study was to analyze the mechanism of the neuroprotective effect of hydroxytyrosol (HT) in an experimental model of hypoxia-reoxygenation in rat brain slices. After reoxygenation the increase in lactate dehydrogenase efflux was inhibited by HT in a concentration-dependent manner and dose-dependent inhibition after oral administration to rats for 7 days (1, 5 and 10 mg/kg per day). Maximum inhibition was 57.4% in vitro and 38.7% ex vivo. Hydroxytyrosol reduced oxidative stress parameters: it inhibited lipid peroxidation and increased enzymatic activities related with the glutathione system both in vitro and after oral administration to rats. The increase in prostaglandin E2 and interleukin 1β after reoxygenation were inhibited after incubation of brain slices with HT and after oral administration. The accumulation of nitric oxide in brain slices was reduced in a concentration-dependent manner. In conclusion, HT exerts a neuroprotective effect in a model of hypoxia-reoxygenation in rat brain slices, both in vitro and after 7 days of oral administration to rats. HT exerts an antioxidant activity and lowered some inflammatory markers in this model. © 2013 Elsevier Inc.


Munoz-Marin J.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | Munoz-Marin J.,University of Malaga | De La Cruz J.P.,Laboratorio Of Investigaciones Antitromboticas E Isquemia Tisular Liait | De La Cruz J.P.,University of Malaga | And 13 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

This study was designed to determine whether the oral administration of hydroxytyrosol (HT) alkyl ether derivatives has a neuroprotective effect in rats. The animals were treated for 7 days with HT or ethyl, butyl, hexyl, octyl, and dodecyl HT ether. A method of in vitro hypoxia-reoxygenation in brain slices was used. Hexyl, octyl, and dodecyl HT derivatives reduced brain cell death (LDH efflux). Lipid peroxidation and nitrite concentrations were inhibited most by hexyl, octyl, and dodecyl derivatives. Concentrations of 3-nitrotyrosine were reduced by HT butyl, hexyl, octyl, and dodecyl ether derivatives. Interleukin-1β was significantly reduced in brain slices from rats treated with all HT ether derivatives. LDH efflux showed a linear correlation with brain concentrations of lipid peroxides, nitrites plus nitrates, and interleukin 1β. The reduction in oxidative and nitrosative stress and decreased production of pro-inflammatory interleukins may be the basis for the observed neuroprotective effects. © 2012 American Chemical Society.

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