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Las Palmas de Gran Canaria, Spain

Brandariz-Fontes C.,CSIC - Donana Biological Station | Brandariz-Fontes C.,University of Panama | Leonard J.A.,EBD Group | Vega-Pla J.L.,Laboratorio Of Investigacion Aplicada | And 4 more authors.
PLoS ONE | Year: 2013

Several studies based on a variety of genetic markers have attempted to establish the origins of horse domestication. Thus far a discrepancy between the results of mitochondrial DNA analysis, which show high levels of diversity, and results from the Y-chromosome, with almost no genetic variability, has been identified. Most previous work on the horse Y-chromosome has focused on widespread, popular breeds or local Asian breeds. It is possible that these breeds represent a reduced set of the genetic variation present in the species. Additional genetic variation may be present in local breeds and ancient feral populations, such as the Retuertas horse in Spain. In this study we analyzed the Y-chromosome of the Retuertas horse, a feral horse population on the Iberian Peninsula that is at least several hundred years old, and whose genetic diversity and morphology suggests that it has been reproductively isolated for a long time. Data from the Retuertas horse was compared to another 11 breeds from the region (Portugal, Spain and France) or likely of Iberian origin, and then to data from 15 more breeds from around the globe. We sequenced 31 introns, Zinc finger Y-chromosomal protein (ZFY) and anonymous Y-linked fragments and genotyped 6 microsatellite loci found on the Y-chromosome. We found no sequence variation among all individuals and all breeds studied. However, fifteen differences were discovered between our data set and reference sequences in GenBank. We show that these likely represent errors within the deposited sequences, and suggest that they should not be used as comparative data for future projects. © 2013 Brandariz-Fontes et al. Source

Aviles D.,Technical University of Ambato | Aviles D.,University of Cordoba, Spain | Landi V.,University of Cordoba, Spain | Vega-Pla J.L.,Laboratorio Of Investigacion Aplicada | Martinez A.,University of Cordoba, Spain
Italian Journal of Animal Science | Year: 2015

The domestic guinea pig is a valuable genetic resource because it is part of local folklore and food tradition in many South American countries. The economic importance of the guinea pig is due to its high feed efficiency and the quality of animal protein produced. For these reasons, our study is aimed to design a complete dinucleotide microsatellite marker set following international recommendation to assess the genetic diversity and genealogy management of guinea pigs. We selected a total of 20 microsatellites, looking for laboratory efficiency and good statistical parameters. The set was tested in 100 unrelated individuals of guinea pigs from Ecuador, Peru, Colombia, Bolivia and Spain. Our results show a high degree of polymorphisms with a total of 216 alleles and a mean number of 10.80±3.49 for markers with a combined exclusion probability of 0.99. © D. Aviles et al., 2015. Source

Miro-Arias M.,University of Cordoba, Spain | Vallecillo A.,University of Cordoba, Spain | Leon J.M.,Delegacion de Medio Ambiente y Promocion Agropecuario | Vega-Pla J.L.,Laboratorio Of Investigacion Aplicada
Archivos de Zootecnia | Year: 2011

Geographical concentration and scarce census of the Retuertas horse force to design a complete conservation plan. One of the used tools as a mean of ex situ conservation was the creation of a germplasm bank. Previously, a study of the seminal quality and its aptitude for freezing was developed. Results were: volume 23.25±12.72 ml; concentration 335.83±135.60x106 spermatozoa/ ml; basal motility 3.83±0.98 (1-5) and progressive motility 60.42±15.42 (0-100%); all of them shown significant differences among stallions. Source

Gama L.T.,University of Lisbon | Martinez A.M.,University of Cordoba, Spain | Carolino I.,INIAV | Landi V.,University of Cordoba, Spain | And 6 more authors.
Genetics Selection Evolution | Year: 2013

Background: Native pig breeds in the Iberian Peninsula are broadly classified as belonging to either the Celtic or the Mediterranean breed groups, but there are other local populations that do not fit into any of these groups. Most of the native pig breeds in Iberia are in danger of extinction, and the assessment of their genetic diversity and population structure, relationships and possible admixture between breeds, and the appraisal of conservation alternatives are crucial to adopt appropriate management strategies. Methods. A panel of 24 microsatellite markers was used to genotype 844 animals representing the 17 most important native swine breeds and wild populations existing in Portugal and Spain and various statistical tools were applied to analyze the results. Results: Genetic diversity was high in the breeds studied, with an overall mean of 13.6 alleles per locus and an average expected heterozygosity of 0.80. Signs of genetic bottlenecks were observed in breeds with a small census size, and population substructure was present in some of the breeds with larger census sizes. Variability among breeds accounted for about 20% of the total genetic diversity, and was explained mostly by differences among the Celtic, Mediterranean and Basque breed groups, rather than by differences between domestic and wild pigs. Breeds clustered closely according to group, and proximity was detected between wild pigs and the Mediterranean cluster of breeds. Most breeds had their own structure and identity, with very little evidence of admixture, except for the Retinto and Entrepelado varieties of the Mediterranean group, which are very similar. Genetic influence of the identified breed clusters extends beyond the specific geographical areas across borders throughout the Iberian Peninsula, with a very sharp transition from one breed group to another. Analysis of conservation priorities confirms that the ranking of a breed for conservation depends on the emphasis placed on its contribution to the between- and within-breed components of genetic diversity. Conclusions: Native pig breeds in Iberia reveal high levels of genetic diversity, a solid breed structure and a clear organization in well-defined clusters. © 2013 Gama et al.; licensee BioMed Central Ltd. Source

Promerova M.,Uppsala University | Andersson L.S.,Swedish University of Agricultural Sciences | Juras R.,Texas A&M University | Penedo M.C.T.,University of California at Davis | And 17 more authors.
Animal Genetics | Year: 2014

For centuries, domestic horses have represented an important means of transport and served as working and companion animals. Although their role in transportation is less important today, many horse breeds are still subject to intense selection based on their pattern of locomotion. A striking example of such a selected trait is the ability of a horse to perform additional gaits other than the common walk, trot and gallop. Those could be four-beat ambling gaits, which are particularly smooth and comfortable for the rider, or pace, used mainly in racing. Gaited horse breeds occur around the globe, suggesting that gaitedness is an old trait, selected for in many breeds. A recent study discovered that a nonsense mutation in DMRT3 has a major impact on gaitedness in horses and is present at a high frequency in gaited breeds and in horses bred for harness racing. Here, we report a study of the worldwide distribution of this mutation. We genotyped 4396 horses representing 141 horse breeds for the DMRT3 stop mutation. More than half (2749) of these horses also were genotyped for a SNP situated 32 kb upstream of the DMRT3 nonsense mutation because these two SNPs are in very strong linkage disequilibrium. We show that the DMRT3 mutation is present in 68 of the 141 genotyped horse breeds at a frequency ranging from 1% to 100%. We also show that the mutation is not limited to a geographical area, but is found worldwide. The breeds with a high frequency of the stop mutation (>50%) are either classified as gaited or bred for harness racing. © 2014 Stichting International Foundation for Animal Genetics. Source

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