Laboratorio Of Inmunobiologia
Laboratorio Of Inmunobiologia
Montiel-Davalos A.,Instituto Nacional Of Cardiologia Ignacio Chavez |
Gonzalez-Villava A.,Laboratorio Of Inmunobiologia |
Rodriguez-Lara V.,Laboratorio Of Inmunobiologia |
Montano L.F.,Laboratorio Of Inmunobiologia |
And 2 more authors.
Journal of Applied Toxicology | Year: 2012
Vanadium is a transition metal released into the atmosphere, as air-suspended particles, as a result of the combustion of fossil fuels and some metallurgic industry activities. Air-suspended particle pollution causes inflammation-related processes such as thrombosis and other cardiovascular events. Our aim was to evaluate the effect of vanadium pentoxide (V 2O 5) on endothelial cells since they are key participants in the pathogenesis of several cardiovascular and inflammatory diseases. Cell adhesion, the expression of adhesion molecules and oxidative stress, as well as proliferation, morphology and cell death of human umbilical vein endothelial cells (HUVECs) exposed to V 2O 5, were evaluated. Vanadium pentoxide at a 3.12μg cm -2 concentration induced an enhanced adhesion of the U937 macrophage cell line to HUVECs, owing to an increased expression of late adhesion molecules. HUVECs exposed to V 2O 5 showed an increase in ROS and nitric oxide production, and a diminished proliferation. These changes in vanadium-treated HUVECs were accompanied by severe morphological changes and apoptotic cell death. Vanadium pentoxide induced serious endothelial cell damage, probably related to the increased cardiovascular morbidity and mortality observed in individuals living in highly air-polluted areas. © 2011 John Wiley & Sons, Ltd.
Dominguez-Melendez V.,National Autonomous University of Mexico |
Silvestre-Santana O.,National Autonomous University of Mexico |
Aguiniga-Sanchez I.,National Autonomous University of Mexico |
Martinez L.,National Autonomous University of Mexico |
And 8 more authors.
Inflammation Research | Year: 2012
Objective and design Sodium caseinate (CasNa) induces differentiation and M-CSF production in mouse band granulocytes in vitro; however, it is not yet known if this molecule can also induce the proliferation and activation of the granulocyte lineage in vivo. In this work we evaluated the induction in vivo of granulopoiesis and the activation of granulocytes in mice treated with CasNa. Material or subjects BALB/c male mice 8-12 weeks old were used. Treatment The animals were inoculated intraperitoneally with 1 ml of CasNa (10% in PBS p/v) four times (every 48 h). Methods Granulocyte proliferation was evaluated by flow cytometry; activation was evaluated by phagocytic indices. The cytokine was measured using an ELISA assay. Results We show that CasNa increased bone marrow granulopoiesis percentage (38.35 ± 10.88 vs. 64.94 ± 34.14 BrdU±/Gr-1± cells) and the granulocytes generated presented increased phagocytic indices (0.3 ± 0.1 vs. 0.6 ± 0.11, p\0.05). We also show that G-CSF (974 ± 411 vs. 3189 ± 350 pg/ml, p\0.05) and GM-CSF increased in serum, but only G-CSF in bone marrow plasma. Conclusions CasNa induces granulopoiesis with functional granulocytes, suggesting that this molecule could be an innate immune system activator. copyright © Springer Basel AG 2012.
Monroy-Garcia A.,Laboratorio Of Inmunobiologia |
Gomez-Lim M.A.,CINVESTAV |
Weiss-Steider B.,Laboratorio Of Inmunobiologia |
Hernandez-Montes J.,Laboratorio Of Inmunobiologia |
And 4 more authors.
Archives of Virology | Year: 2014
HPV L1-based virus-like particles vaccines (VLPs) efficiently induce temporary prophylactic activity through the induction of neutralizing antibodies; however, VLPs that can provide prophylactic as well as therapeutic properties for longer periods of time are needed. For this purpose, we generated a novel HPV 16 L1-based chimeric virus-like particle (cVLP) produced in plants that contains a string of T-cell epitopes from HPV 16 E6 and E7 fused to its C-terminus. In the present study, we analyzed the persistence of specific IgG antibodies with neutralizing activity induced by immunization with these cVLPs, as well as their therapeutic potential in a tumor model of C57BL/6 mice. We observed that these cVLPs induced persistent IgG antibodies for over 12 months, with reactivity and neutralizing activity for VLPs composed of only the HPV-16 L1 protein. Efficient protection for long periods of time and inhibition of tumor growth induced by TC-1 tumor cells expressing HPV-16 E6/E7 oncoproteins, as well as significant tumor reduction (57 %), were observed in mice immunized with these cVLPs. Finally, we discuss the possibility that chimeric particles of the type described in this work may be the basis for developing HPV prophylactic and therapeutic vaccines with high efficacy. © 2013 Springer-Verlag Wien.
Partida-Perez M.,Institute Genetica Humana |
De La Luz Ayala-Madrigal M.,Institute Genetica Humana |
Peregrina-Sandoval J.,Laboratorio Of Inmunobiologia |
MacIas-Gomez N.,Laboratorio Of Genetica Humana |
And 7 more authors.
Cancer Biomarkers | Year: 2010
Leptin and adiponectin are cytokines produced by adipose tissue with opposite effects on tumor growth: the former stimulate whereas the latter inhibit it. The objective was to analyze the association of LEP A19G and ADIPOQ+45 T/G and +276 G/T polymorphisms in Mexican patients with colorectal cancer (CRC). 68 unrelated patients with CRC (study group) and 102 blood donors (control group); all subjects were Mestizos from western Mexico. The polymorphisms were established by PCR-RFLP on DNA samples obtained from peripheral blood. The LEP A19G polymorphism showed significant differences between CRC patients and control group (p= 0.01 for G/A genotype and p= 0.02 for the recessive model G/G +G/A); yet, in the analysis stratified by gender, this difference remained significant only in males. The ADIPOQ polymorphisms did not shown any significant differences. Our results suggest that the A19G LEP polymorphism is associated with CRC in Mexican patients. © 2010-IOS Press and the authors. All rights reserved.
Uribe-Campero L.,CINVESTAV |
Monroy-Garcia A.,Laboratorio Of Inmunobiologia |
Duran-Meza A.L.,Autonomous University of San Luis Potosi |
Villagrana-Escareno M.V.,Autonomous University of San Luis Potosi |
And 4 more authors.
Research in Veterinary Science | Year: 2015
Porcine reproductive and respiratory syndrome virus (PRRSV) significantly affects the swine industry worldwide. An efficient, protective vaccine is still lacking. Here, we report for the first time the generation and purification of PRRSV virus like particles (VLPs) by expressing GP5, M and N genes in Nicotiana silvestris plants. The particles were clearly visible by transmission electron microscopy (TEM) with a size of 60-70 nm. Hydrodynamic diameter of the particles was obtained and it was confirmed that the VLPs had the appropriate size for PRRS virions and that the VLPs were highly pure. By measuring the Z potential we described the electrophoretic mobility behavior of VLPs and the best conditions for stability of the VLPs were determined. The particles were immunogenic in mice. A western blot of purified particles allowed detection of three coexpressed genes.These VLPs may serve as a platform to develop efficient PRRSV vaccines. © 2015 Elsevier Ltd.
Reyes Garcia M.G.,Laboratorio Of Inmunobiologia |
Garcia Tamayo F.,Laboratorio Of Inmunobiologia
BioMed Research International | Year: 2013
T lymphocytes from the immune system are bone marrow-derived cells whose development and activities are carefully supervised by two sets of accessory cells. In the thymus, the immature young T lymphocytes are engulfed by epithelial "nurse cells" and retained in vacuoles, where most of them (95%) are negatively selected and removed when they have an incomplete development or express high affinity autoreactive receptors. The mature T lymphocytes that survive to this selection process leave the thymus and are controlled in the periphery by another subpopulation of accessory cells called "regulatory cells," which reduce any excessive immune response and the risk of collateral injuries to healthy tissues. By different times and procedures, nurse cells and regulatory cells control both the development and the functions of T lymphocyte subpopulations. Disorders in the T lymphocytes development and migration have been observed in some parasitic diseases, which disrupt the thymic microenvironment of nurse cells. In other cases, parasites stimulate rather than depress the functions of regulatory T cells decreasing T-mediated host damages. This paper is a short review regarding some features of these accessory cells and their main interactions with T immature and mature lymphocytes. The modulatory role that neurotransmitters and hormones play in these interactions is also revised. © 2013 María Guadalupe Reyes García and Fernando García Tamayo.
Moreno-Eutimio M.A.,Laboratorio Of Inmunobiologia |
Moreno-Eutimio M.A.,National Polytechnic Institute of Mexico |
Acosta-Altamirano G.,National Polytechnic Institute of Mexico
Cirugia y Cirujanos | Year: 2014
Sedentary lifestyle leads to the accumulation of visceral fat. This is accompanied by the infiltration of immune cells with pro-inflammatory characteristics in adipose tissue, causing an increased release of cytokines and generating a low-grade inflammatory state. It has been associated with the development of insulin resistance, atherosclerosis, neurodegeneration, and development of tumors. Exercise can be used as a treatment to improve symptoms of many of these conditions because it promotes an anti-inflammatory effect. In this review we analyze the pro-inflammatory factors present in obesity and the induction of antiinflammatory factors that occur with exercise.
PubMed | CINVESTAV, University of Guanajuato, Laboratorio Of Inmunobiologia, Research Center En Alimentacion sarrollo Ac and Autonomous University of San Luis Potosi
Type: | Journal: Research in veterinary science | Year: 2015
Porcine reproductive and respiratory syndrome virus (PRRSV) significantly affects the swine industry worldwide. An efficient, protective vaccine is still lacking. Here, we report for the first time the generation and purification of PRRSV virus like particles (VLPs) by expressing GP5, M and N genes in Nicotiana silvestris plants. The particles were clearly visible by transmission electron microscopy (TEM) with a size of 60-70 nm. Hydrodynamic diameter of the particles was obtained and it was confirmed that the VLPs had the appropriate size for PRRS virions and that the VLPs were highly pure. By measuring the Z potential we described the electrophoretic mobility behavior of VLPs and the best conditions for stability of the VLPs were determined. The particles were immunogenic in mice. A western blot of purified particles allowed detection of three coexpressed genes. These VLPs may serve as a platform to develop efficient PRRSV vaccines.