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De Melo J.F.,Compiegne University of Technology | De Melo J.F.,Federal University of Pernambuco | De Melo J.F.,Laboratorio Of Imunopatologia Keizo Asami | Aloulou N.,Compiegne University of Technology | And 6 more authors.
European Journal of Nutrition | Year: 2011

Aim To investigate the effects of a neonatal low-protein diet on the morphology of myotubes in culture and the expression of key proteins that regulate myogenesis in young and adult rats. Methods Male Wistar rats (n = 18) were suckled by mothers fed diets containing 17% protein (controls, C) or 8% protein (undernourished, UN). All rats were fed a normal protein diet after weaning. Muscles were removed from the legs of 42-, 60- and 90-day-old rats. Muscle cells were cultured to assess cell number, morphology and the expression of major proteins involved in myogenesis (Pax7, cadherins, β1 integrin, IL-4Rα and myogenin) by western blotting. IL-4 levels in culture supernatants were measured by ELISA. Results Offspring from mothers fed a low-protein diet showed a lower body weight gain. Cell number and myotube expansion were reduced in cultured muscle cells from UN, but the expression of myogenic marker proteins was unaltered. Conclusions Dietary restriction during lactation had no impact on the synthesis of myogenic marker proteins, and myocyte differentiation occurred normally in the muscles of offspring aged 42, 60 or 90 days. Nevertheless, the number and morphology of the myotubes are altered. © Springer-Verlag 2010. Source

Soria F.,National University of Salta | Ellenrieder G.,National University of Salta | Oliveira G.B.,Federal University of Reconcavo da Bahia | Cabrera M.,National University of Salta | Carvalho Jr. L.B.,Laboratorio Of Imunopatologia Keizo Asami
Applied Microbiology and Biotechnology | Year: 2012

α-l-Rhamnosidase from Aspergillus terreus was covalently immobilized on the following ferromagnetic supports: polyethylene terephthalate (Dacron-hydrazide), polysiloxane/polyvinyl alcohol (POS/PVA), and chitosan. The powdered supports were magnetized by thermal coprecipitation method using ferric and ferrous chlorides, and the immobilization was carried out via glutaraldehyde. The activity of the Dacron-hydrazide (0.53 nkat/μg of protein) and POS/PVA (0.59 nkat/μg of protein) immobilized enzyme was significantly higher than that found for the chitosan derivative (0.06 nkat/μg of protein). The activity-pH and activity-temperature profiles for all immobilized enzymes did not show difference compared to the free enzyme, except the chitosan derivative that presented higher maximum temperature at 65°C. The Dacron-hydrazide derivative thermal stability showed a similar behavior of the free enzyme in the temperature range of 40-70°C. The POS/PVA and chitosan derivatives were stable up to 60°C, but were completely inactivated at 70°C. The activity of the preparations did not appreciably decrease after ten successive reuses. Apparent K m of α-l-rhamnosidase immobilized on magnetized Dacron-hydrazide (1.05∈±∈0.22 mM), POS/PVA (0.57∈±∈0.09 mM), and chitosan (1.78∈±∈0.24 mM) were higher than that estimated for the soluble enzyme (0.30∈±∈0.03 mM). The Dacron-hydrazide enzyme derivative showed better performance than the free enzyme to hydrolyze 0.3% narigin (91% and 73% after 1 h, respectively) and synthesize rhamnosides (0.116 and 0.014 mg narirutin after 1 h, respectively). © 2011 Springer-Verlag. Source

Silva H.D.,IBB Institute for Biotechnology And Bioengineering | Cerqueira M.A.,IBB Institute for Biotechnology And Bioengineering | Souza B.W.S.,IBB Institute for Biotechnology And Bioengineering | Ribeiro C.,IBB Institute for Biotechnology And Bioengineering | And 6 more authors.
Journal of Food Engineering | Year: 2011

Nanoemulsions of β-carotene were prepared using a high-energy emulsification-evaporation technique based on a 23 level factorial design. Results show that it is possible to obtain dispersions at a nanoscale range. Process parameters such as time and shear rate of homogenization affected significantly particle size distribution in terms of volume-weighted mean diameter and surface-weighted mean diameter. The obtained nanoemulsions presented a volume-surface diameter ranging from 9 to 280 nm immediately after the production of particles, displaying in all cases a monomodal size distribution. Those nanoemulsions showed a good physical stability during 21 days storage. The stability was evaluated by the maintenance of size distribution. However, β-carotene retention inside the micelles and color were affected by storage. Processing conditions also influenced storage stability. © 2010 Elsevier Ltd. All rights reserved. Source

Albuquerque A.C.C.,Bairro Universitario 55016 400 Caruaru Pe | Silva D.M.,Bairro Universitario 55016 400 Caruaru Pe | Rabelo D.C.C.,Bairro Universitario 55016 400 Caruaru Pe | Lucena W.A.T.,Bairro Universitario 55016 400 Caruaru Pe | And 3 more authors.
Ciencia e Saude Coletiva | Year: 2014

Prison inmates are more vulnerable to HIV and other Sexually Transmitted Infections (STIs) due to risk factors such as needle sharing and unprotected sex with homosexuals. The aim of this work was to determine the seroprevalence and risk factors associated with the human immunodeficiency virus (HIV-1/2) and syphilis among male inmates in Caruaru, State of Pernambuco, Brazil. A cross-sectional study was performed between May and July 2011, when 1,097 inmates at a prison in Caruaru were assessed by means of interviews and blood sample collection for performing the respective tests. The prevalence was 1.19% for HIV infection and 3.92% for syphilis. HIV infection showed a statistically significant association (p <0.05), with injected drug use, homosexuality and blood transfusions. With respect to HIV status and syphilis, factors related to sex life were statistically significant (p <0.05). The prison population is a high risk group for the diseases investigated. The prevalence rates identified indicate the need to implement prevention programs, helping to contain such diseases in this particular population group. Source

Mourato F.A.,Circulo do Coracao de Pernambuco | Nadruz W.,Laboratorio Of Imunopatologia Keizo Asami | Moser L.R.,Circulo do Coracao de Pernambuco | de Lima Filho J.L.,Laboratorio Of Imunopatologia Keizo Asami | Mattos S.S.,Circulo do Coracao de Pernambuco
American journal of hypertension | Year: 2015

BACKGROUND: The blood pressure to height ratio (BP:HT) has been proposed as a simple method for identifying children with elevated BP. This procedure shows good accuracy for the screening of hypertension in adolescents but less so in younger children. Our aim in this study was to modify the BP:HT ratio and determine if this change would increase accuracy when measuring hypertension during childhood.METHODS: BP levels of 4,327 children (aged 5-12 years) were retrospectively obtained from medical charts. The modified ratio (BT:eHT13) was calculated as: BP/(HT + 7 × (13 - age in years)). Receiver operating characteristic curves were used to estimate cutoff points and the accuracy of the conventional and modified ratio to detect prehypertension and hypertension.RESULTS: The prevalences of prehypertension and hypertension were 3.91% and 5.44%, respectively. In general, BP:eHT13 showed higher sensitivity (ranging from 0.95 to 1.00) and specificity (ranging from 0.80 to 0.98) in detecting prehypertension, level I hypertension, and level II hypertension than BP:HT (sensitivity ranging from 0.91 to 1.00; specificity ranging from 0.59 to 0.89).CONCLUSIONS: The modified BP:eHT13 ratio showed better sensitivity and specificity for the screening of BP abnormalities in children aged 5-12 years. © American Journal of Hypertension, Ltd 2014. All rights reserved. For Permissions, please email: journals.permissions@oup.com. Source

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