Laboratorio Of Imunopatologia Keizo Asami

Rio de Janeiro, Brazil

Laboratorio Of Imunopatologia Keizo Asami

Rio de Janeiro, Brazil

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De Melo J.F.,Compiègne University of Technology | De Melo J.F.,Federal University of Pernambuco | De Melo J.F.,Laboratorio Of Imunopatologia Keizo Asami | Aloulou N.,Compiègne University of Technology | And 6 more authors.
European Journal of Nutrition | Year: 2011

Aim To investigate the effects of a neonatal low-protein diet on the morphology of myotubes in culture and the expression of key proteins that regulate myogenesis in young and adult rats. Methods Male Wistar rats (n = 18) were suckled by mothers fed diets containing 17% protein (controls, C) or 8% protein (undernourished, UN). All rats were fed a normal protein diet after weaning. Muscles were removed from the legs of 42-, 60- and 90-day-old rats. Muscle cells were cultured to assess cell number, morphology and the expression of major proteins involved in myogenesis (Pax7, cadherins, β1 integrin, IL-4Rα and myogenin) by western blotting. IL-4 levels in culture supernatants were measured by ELISA. Results Offspring from mothers fed a low-protein diet showed a lower body weight gain. Cell number and myotube expansion were reduced in cultured muscle cells from UN, but the expression of myogenic marker proteins was unaltered. Conclusions Dietary restriction during lactation had no impact on the synthesis of myogenic marker proteins, and myocyte differentiation occurred normally in the muscles of offspring aged 42, 60 or 90 days. Nevertheless, the number and morphology of the myotubes are altered. © Springer-Verlag 2010.


Neri D.F.M.,Federal University of Vale do São Francisco | Neri D.F.M.,Laboratorio Of Imunopatologia Keizo Asami | Bernardino D.P.B.,Laboratorio Of Imunopatologia Keizo Asami | Bernardino D.P.B.,Federal University of Pernambuco | And 4 more authors.
Applied Catalysis A: General | Year: 2011

Bovine milk xanthine oxidase (XOD, E.C. 1.17.3.2) was covalently immobilized, via glutaraldehyde, on magnetic polysiloxane-polyvinyl alcohol (mPOS-PVA) particles yielding a preparation containing 9.5 ± 0.5 μg of protein per mg of support and specific activity of 36.3 ± 7.8 mU/mg of protein (55.0 ± 11.7% of the free enzyme). Optimal pH (8.8) and temperature (60 °C) were slightly higher than those established for the free enzyme (8.2 and 55 °C, respectively). No decrease of activity was observed after five reuses and only 17% was lost at the tenth reuse. The apparent Michaelis constant estimated for the mPOS-PVA-XOD (8.86 ± 0.88 μM) was not statistically different from the free enzyme (7.48 ± 1.01 μM). The 6-mercaptopurine oxidation catalyzed by the mPOS-PVA-XOD followed the same pathway described for the free enzyme, namely, 6-mercaptopurine → 6-mercapto-8-hydroxypurine → 6-thiouric acid, and no 6-thioxanthine was formed. © 2011 Elsevier B.V.


Lira M.C.B.,Federal University of Pernambuco | Lira M.C.B.,Laboratorio Of Imunopatologia Keizo Asami | Lira M.C.B.,University Paris - Sud | Lira M.C.B.,French National Center for Scientific Research | And 11 more authors.
European Journal of Pharmaceutics and Biopharmaceutics | Year: 2011

The aim was to synthesize and characterize fucoidan-coated poly(isobutylcyanoacrylate) nanoparticles. The nanoparticles were prepared by anionic emulsion polymerization (AEP) and by redox radical emulsion polymerization (RREP) of isobutylcyanoacrylate using fucoidan as a new coating material. The nanoparticles were characterized, and their cytotoxicity was evaluated in vitro on J774 macrophage and NIH-3T3 fibroblast cell lines. Cellular uptake of labeled nanoparticles was investigated by confocal fluorescence microscopy. Results showed that both methods were suitable to prepare stable formulations of fucoidan-coated PIBCA nanoparticles. Stable dispersions of nanoparticles were obtained by AEP with up to 100% fucoidan as coating material. By the RREP method, stable suspensions of nanoparticles were obtained with only up to 25% fucoidan in a blend of polysaccharide composed of dextran and fucoidan. The zeta potential of fucoidan-coated nanoparticles was decreased depending on the percentage of fucoidan. It reached the value of -44 mV for nanoparticles prepared by AEP with 100% of fucoidan. Nanoparticles made by AEP appeared more than four times more cytotoxic (IC 50 below 2 μg/mL) on macrophages J774 than nanoparticles made by RREP (IC 50 above 9 μg/mL). In contrast, no significant difference in cytotoxicity was highlighted by incubation of the nanoparticles with a fibroblast cell line. On fibroblasts, both types of nanoparticles showed similar cytotoxicity. Confocal fluorescence microscopy observations revealed that all types of nanoparticles were taken up by both cell lines. The distribution of the fluorescence in the cells varied greatly with the type of nanoparticles. © 2011 Elsevier B.V. All rights reserved.


PubMed | Hospital Casa Of Saude Bom Jesus, Laboratorio Of Imunopatologia Keizo Asami and Laboratorio Escola
Type: Journal Article | Journal: Ciencia & saude coletiva | Year: 2014

Prison inmates are more vulnerable to HIV and other Sexually Transmitted Infections (STIs) due to risk factors such as needle sharing and unprotected sex with homosexuals. The aim of this work was to determine the seroprevalence and risk factors associated with the human immunodeficiency virus (HIV-1/2) and syphilis among male inmates in Caruaru, State of Pernambuco, Brazil. A cross-sectional study was performed between May and July 2011, when 1,097 inmates at a prison in Caruaru were assessed by means of interviews and blood sample collection for performing the respective tests. The prevalence was 1.19% for HIV infection and 3.92% for syphilis. HIV infection showed a statistically significant association (p <0.05), with injected drug use, homosexuality and blood transfusions. With respect to HIV status and syphilis, factors related to sex life were statistically significant (p <0.05). The prison population is a high risk group for the diseases investigated. The prevalence rates identified indicate the need to implement prevention programs, helping to contain such diseases in this particular population group.


PubMed | University of Santo Amaro and Laboratorio Of Imunopatologia Keizo Asami
Type: | Journal: Clinical neurology and neurosurgery | Year: 2016

Cerebral toxoplasmosis is the most common cause of space occupying brain lesion in patients with HIV/AIDS in Brazil. In the post-HAART era, it is responsible for high rates of morbidity and mortality worldwide.This study consists of a case series of 56 patients diagnosed with cerebral toxoplasmosis whose clinical features, brain imaging and cerebrospinal fluid aspects were analyzed.Cerebral toxoplasmosis led to the diagnosis of infection by the human immunodeficiency virus (HIV) in 27 (48.2%) of the patients, while 29 (51.2%) others already knew to be HIV seropositive. However, at the time of diagnosis of cerebral toxoplasmosis, only 9 (16.6%) reported being under antiretroviral therapy and 5 (8.9%) were receiving primary prophylaxis for toxoplasmosis. Headache, strength deficit and fever were the most frequent signs and symptoms throughout the study. Fifty-three patients showed changes consistent with toxoplasmosis in CT or MRI. Thirty-four (60.7%) CSF samples were positive in the indirect haemagglutination test and for the reaction of Toxoplasma gondii IgG ELISA, while 31 (55.4%) were positive in the direct haemagglutination test. Fifty (89.3%) patients underwent first-line treatment for toxoplasmosis.Cerebral toxoplasmosis is still a very relevant neurological disease in individuals with AIDS admitted to neurology emergency departments. Early diagnosis and initiation of empiric treatment and antiretroviral therapy are important for good prognosis.


Soria F.,National University of Salta | Ellenrieder G.,National University of Salta | Oliveira G.B.,Federal University of Recôncavo da Bahia | Cabrera M.,National University of Salta | Carvalho Jr. L.B.,Laboratorio Of Imunopatologia Keizo Asami
Applied Microbiology and Biotechnology | Year: 2012

α-l-Rhamnosidase from Aspergillus terreus was covalently immobilized on the following ferromagnetic supports: polyethylene terephthalate (Dacron-hydrazide), polysiloxane/polyvinyl alcohol (POS/PVA), and chitosan. The powdered supports were magnetized by thermal coprecipitation method using ferric and ferrous chlorides, and the immobilization was carried out via glutaraldehyde. The activity of the Dacron-hydrazide (0.53 nkat/μg of protein) and POS/PVA (0.59 nkat/μg of protein) immobilized enzyme was significantly higher than that found for the chitosan derivative (0.06 nkat/μg of protein). The activity-pH and activity-temperature profiles for all immobilized enzymes did not show difference compared to the free enzyme, except the chitosan derivative that presented higher maximum temperature at 65°C. The Dacron-hydrazide derivative thermal stability showed a similar behavior of the free enzyme in the temperature range of 40-70°C. The POS/PVA and chitosan derivatives were stable up to 60°C, but were completely inactivated at 70°C. The activity of the preparations did not appreciably decrease after ten successive reuses. Apparent K m of α-l-rhamnosidase immobilized on magnetized Dacron-hydrazide (1.05∈±∈0.22 mM), POS/PVA (0.57∈±∈0.09 mM), and chitosan (1.78∈±∈0.24 mM) were higher than that estimated for the soluble enzyme (0.30∈±∈0.03 mM). The Dacron-hydrazide enzyme derivative showed better performance than the free enzyme to hydrolyze 0.3% narigin (91% and 73% after 1 h, respectively) and synthesize rhamnosides (0.116 and 0.014 mg narirutin after 1 h, respectively). © 2011 Springer-Verlag.


Silva H.D.,IBB Institute for Biotechnology And Bioengineering | Cerqueira M.A.,IBB Institute for Biotechnology And Bioengineering | Souza B.W.S.,IBB Institute for Biotechnology And Bioengineering | Ribeiro C.,IBB Institute for Biotechnology And Bioengineering | And 6 more authors.
Journal of Food Engineering | Year: 2011

Nanoemulsions of β-carotene were prepared using a high-energy emulsification-evaporation technique based on a 23 level factorial design. Results show that it is possible to obtain dispersions at a nanoscale range. Process parameters such as time and shear rate of homogenization affected significantly particle size distribution in terms of volume-weighted mean diameter and surface-weighted mean diameter. The obtained nanoemulsions presented a volume-surface diameter ranging from 9 to 280 nm immediately after the production of particles, displaying in all cases a monomodal size distribution. Those nanoemulsions showed a good physical stability during 21 days storage. The stability was evaluated by the maintenance of size distribution. However, β-carotene retention inside the micelles and color were affected by storage. Processing conditions also influenced storage stability. © 2010 Elsevier Ltd. All rights reserved.


PubMed | Circulo do Coracao de Pernambuco, Laboratorio Of Imunopatologia Keizo Asami and University of Pernambuco
Type: Journal Article | Journal: American journal of hypertension | Year: 2015

The blood pressure to height ratio (BP:HT) has been proposed as a simple method for identifying children with elevated BP. This procedure shows good accuracy for the screening of hypertension in adolescents but less so in younger children. Our aim in this study was to modify the BP:HT ratio and determine if this change would increase accuracy when measuring hypertension during childhood.BP levels of 4,327 children (aged 5-12 years) were retrospectively obtained from medical charts. The modified ratio (BT:eHT13) was calculated as: BP/(HT + 7 (13 - age in years)). Receiver operating characteristic curves were used to estimate cutoff points and the accuracy of the conventional and modified ratio to detect prehypertension and hypertension.The prevalences of prehypertension and hypertension were 3.91% and 5.44%, respectively. In general, BP:eHT13 showed higher sensitivity (ranging from 0.95 to 1.00) and specificity (ranging from 0.80 to 0.98) in detecting prehypertension, level I hypertension, and level II hypertension than BP:HT (sensitivity ranging from 0.91 to 1.00; specificity ranging from 0.59 to 0.89).The modified BP:eHT13 ratio showed better sensitivity and specificity for the screening of BP abnormalities in children aged 5-12 years.


Albuquerque A.C.C.,Bairro Universitario 55016 400 Caruaru Pe | Silva D.M.,Bairro Universitario 55016 400 Caruaru Pe | Rabelo D.C.C.,Bairro Universitario 55016 400 Caruaru Pe | Lucena W.A.T.,Bairro Universitario 55016 400 Caruaru Pe | And 3 more authors.
Ciencia e Saude Coletiva | Year: 2014

Prison inmates are more vulnerable to HIV and other Sexually Transmitted Infections (STIs) due to risk factors such as needle sharing and unprotected sex with homosexuals. The aim of this work was to determine the seroprevalence and risk factors associated with the human immunodeficiency virus (HIV-1/2) and syphilis among male inmates in Caruaru, State of Pernambuco, Brazil. A cross-sectional study was performed between May and July 2011, when 1,097 inmates at a prison in Caruaru were assessed by means of interviews and blood sample collection for performing the respective tests. The prevalence was 1.19% for HIV infection and 3.92% for syphilis. HIV infection showed a statistically significant association (p <0.05), with injected drug use, homosexuality and blood transfusions. With respect to HIV status and syphilis, factors related to sex life were statistically significant (p <0.05). The prison population is a high risk group for the diseases investigated. The prevalence rates identified indicate the need to implement prevention programs, helping to contain such diseases in this particular population group.


Mourato F.A.,Circulo do Coracao de Pernambuco | Nadruz W.,Laboratorio Of Imunopatologia Keizo Asami | Moser L.R.,Circulo do Coracao de Pernambuco | de Lima Filho J.L.,Laboratorio Of Imunopatologia Keizo Asami | Mattos S.S.,Circulo do Coracao de Pernambuco
American journal of hypertension | Year: 2015

BACKGROUND: The blood pressure to height ratio (BP:HT) has been proposed as a simple method for identifying children with elevated BP. This procedure shows good accuracy for the screening of hypertension in adolescents but less so in younger children. Our aim in this study was to modify the BP:HT ratio and determine if this change would increase accuracy when measuring hypertension during childhood.METHODS: BP levels of 4,327 children (aged 5-12 years) were retrospectively obtained from medical charts. The modified ratio (BT:eHT13) was calculated as: BP/(HT + 7 × (13 - age in years)). Receiver operating characteristic curves were used to estimate cutoff points and the accuracy of the conventional and modified ratio to detect prehypertension and hypertension.RESULTS: The prevalences of prehypertension and hypertension were 3.91% and 5.44%, respectively. In general, BP:eHT13 showed higher sensitivity (ranging from 0.95 to 1.00) and specificity (ranging from 0.80 to 0.98) in detecting prehypertension, level I hypertension, and level II hypertension than BP:HT (sensitivity ranging from 0.91 to 1.00; specificity ranging from 0.59 to 0.89).CONCLUSIONS: The modified BP:eHT13 ratio showed better sensitivity and specificity for the screening of BP abnormalities in children aged 5-12 years. © American Journal of Hypertension, Ltd 2014. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

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