Laboratorio Of Higado

D.F., Mexico

Laboratorio Of Higado

D.F., Mexico
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Gutierrez-Herrera E.,National Autonomous University of Mexico | Perez-Garcia A.,Servicio de Cirugia Experimental | Perez-Garcia A.,Laboratorio Of Higado | Ortega-Martinez A.,Harvard University | And 2 more authors.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2017

Non-subjective, minimally-invasive, and quantifying techniques may support development and evaluation of a fibrosis regression treatment. The build-up of extracellular matrix in liver fibrosis may result on changes of the endogenous fluorescence of tissue. In this work, we evaluate the fluorescence excitation/emission matrix in the UV range for several bulk samples of murine hepatic tissue preserved in different media. Chemical changes on tissue, caused by formaldehyde preservation, alter the endogenous fluorescence spectra. To avoid these drawbacks, phosphate-buffered saline (PBS) or Iscove's Modified Dulbecco's Medium were used. PBS buffer showed to be the less harmful and cost-effective preservation medium to study the endogenous fluorescence in fibrotic tissue. © 2017 SPIE.


Duarte-Rojo A.,Instituto Nacional Of Ciencias Medicas Y Nutricion Salvador Zubiran | Sosa-Lozano L.A.,Instituto Nacional Of Ciencias Medicas Y Nutricion Salvador Zubiran | Saul A.,Instituto Nacional Of Ciencias Medicas Y Nutricion Salvador Zubiran | Herrera-Caceres J.O.,Instituto Nacional Of Ciencias Medicas Y Nutricion Salvador Zubiran | And 3 more authors.
Alimentary Pharmacology and Therapeutics | Year: 2010

Background Obesity increases the risk for severe acute pancreatitis, although abdominal obesity may be a better prognostic marker. Aim To determine if a single anthropometric parameter best predicts severe acute pancreatitis and correlates with intra-abdominal fat. Methods Ninety-nine patients with acute pancreatitis were studied prospectively. Anthropometry included body mass index (BMI) and girths (umbilical/minimum waist, iliac/trochanter hip, thigh). Several waist-to-hip/waist-to-thigh ratios (WHR/WTR) were constructed. A CT-scan with calculation of cross-sectional abdominal fat areas was obtained in 37 cases. Results Severe acute pancreatitis occurred in 25 patients. Waist circumference (WC), WHR and WTR - all using the umbilical reference - most accurately predicted severe acute pancreatitis. Only umbilical WC was retained in multivariate analysis: the risk for severe acute pancreatitis increased 16% with every 1 cm (OR 1.16, 95%CI: 1.1-1.3). Abdominal obesity caused a 6-fold increase in risk. Umbilical WC correlated best with subcutaneous fat area (r = 0.791, P < 0.001), whereas WHR with intra-abdominal (r = 0.594, P < 0.001). Conclusions Abdominal obesity according to umbilical WC is a better predictor for development of severe acute pancreatitis than BMI, minimum WC, WHR and WTR. The protocol for anthropometry must be standardized as it may affect results. Both subcutaneous and intra-abdominal fat appears to affect the likelihood of a severe outcome. © 2010 Blackwell Publishing Ltd.

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