Laboratorio Of Genomica Funcional

Laboratorio Of Genomica Funcional

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Pacheco-Lugo L.,Federal University of Paraná | Pacheco-Lugo L.,Simón Bolívar University of Colombia | Diaz-Olmos Y.,Laboratorio Of Genomica Funcional | Saenz-Garcia J.,Federal University of Paraná | And 2 more authors.
Parasitology International | Year: 2017

New opportunities have raised to study the gene function approaches of Trypanosoma cruzi after its genome sequencing in 2005. Functional genomic approaches in Trypanosoma cruzi are challenging due to the reduced tools available for genetic manipulation, as well as to the reduced efficiency of the transient transfection conducted through conventional methods. The Amaxa nucleofector device was systematically tested in the present study in order to improve the electroporation conditions in the epimastigote forms of T. cruzi. The transfection efficiency was quantified using the green fluorescent protein (GFP) as reporter gene followed by cell survival assessment. The herein used nucleofection parameters have increased the survival rates (> 90%) and the transfection efficiency by approximately 35%. The small amount of epimastigotes and DNA required for the nucleofection can turn the method adopted here into an attractive tool for high throughput screening (HTS) applications, and for gene editing in parasites where genetic manipulation tools remain relatively scarce. © 2017 Elsevier B.V.


Mangeon A.,Laboratorio Of Genomica Funcional | Junqueira R.M.,Laboratorio Of Genomica Funcional | Sachetto-Martins G.,Laboratorio Of Genomica Funcional
Plant Signaling and Behavior | Year: 2010

The first plant glycine-rich proteins (GRPs) have been isolated more than 20 years ago based on their specific expression pattern and/or modulation by several biotic and abiotic factors. This superfamily is characterized by the presence of a glycinerich domain arranged in (Gly)n-X repeats. The presence of additional motifs, as well as the nature of the glycine repeats, groups them in different classes. The diversity in structure as well as in expression pattern, modulation and sub cellular localization have always indicated that these proteins, although classified as members of the same superfamily, would perform different functions in planta. Only now, two decades later, with the first functional characterizations of plant GRPs their involvement in diverse biological and biochemical processes are being uncovered. Here, we review the so far ascribed functions of plant GRPs. © 2010 Landes Bioscience.


Salas A.,Autonomous University of Baja California | Diaz F.,Research Center Cientifica Educacion Superior Of Ensenada | Re A.D.,Research Center Cientifica Educacion Superior Of Ensenada | Galindosanchez C.E.,Laboratorio Of Genomica Funcional | And 5 more authors.
Journal of Shellfish Research | Year: 2014

The preferred temperature of Tegula regina was determined in a horizontal thermal gradient with organisms acclimated to 16°C, 19°C, and 22°C using to acute method, with a preferred temperature of 19.7 ± 1.8°C for the day cycle and 18.8 ± 1.2°C for the night cycle. The final preferred temperature determined for marine snails was 19.2 ± 1.5°C. The displacement velocity decreased to an interval from 99.1 cm/h to 62.5 cm/h. During the third hour, when organisms detected the preferred temperature, the velocity diminished gradually from 25.8 cm/h to 7.5 cm/h. Critical temperature maxima (CTMax), which refers to the temperature at which at least 50% of the experimental group displays a loss of attachment, was measured at three acclimation temperatures (16°C, 19°C, and 22°C). At the acclimation temperature (16°C), 50% of the experimental group had an attachment loss at a CTMax of 29.3°C. At a warmer acclimation temperature (22°C), the observed CTMax was 31.2°C. Marine snail oxygen consumption rate increased significantly (P < 0.05, 31%) from 16°C to 22°C.


PubMed | Simón Bolívar University of Colombia, Federal University of Paraná and Laboratorio Of Genomica Funcional
Type: | Journal: Parasitology international | Year: 2017

New opportunities have raised to study the gene function approaches of Trypanosoma cruzi after its genome sequencing in 2005. Functional genomic approaches in Trypanosoma cruzi are challenging due to the reduced tools available for genetic manipulation, as well as to the reduced efficiency of the transient transfection conducted through conventional methods. The Amaxa nucleofector device was systematically tested in the present study in order to improve the electroporation conditions in the epimastigote forms of T. cruzi. The transfection efficiency was quantified using the green fluorescent protein (GFP) as reporter gene followed by cell survival assessment. The herein used nucleofection parameters have increased the survival rates (>90%) and the transfection efficiency by approximately 35%. The small amount of epimastigotes and DNA required for the nucleofection can turn the method adopted here into an attractive tool for high throughput screening (HTS) applications, and for gene editing in parasites where genetic manipulation tools remain relatively scarce.

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