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Pliego-Cardenas R.,Metropolitan Autonomous University | Pliego-Cardenas R.,Laboratorio Of Genetica Y Biologia Molecular | Hochberg F.G.,Santa Barbara Museum of Natural History | Leon F.J.G.D.,Laboratorio Of Ecologia Molecular | Barriga-Sosa I.D.L.A.,Laboratorio Of Genetica Y Biologia Molecular
Journal of Shellfish Research | Year: 2014

The octopuses Octopus hubbsorum and Octopus mimus are two species of octopuses that inhabit shallow waters of the eastern Pacific Ocean. The former species is found in the north, from the Gulf of California to Oaxaca, Mexico, whereas O. mimus lives in the south, from Peru to Chile. To infer the phylogenetic relationships between these species we used the mitochondrial genes cytochrome oxidase subunits I and III, and r16S with the aim of comparing their genetic distances with those of other Octopus sp. The genetic distance values between both species estimated per gene or concatenated were low (0%-1.6%) in comparison with other interspecific distances values (4.6%-18.4%). Application of 10rule showed there is no overlap between intra-and interspecific octopus distances, whereas the application of the 4rule confirmed that both octopuses belong to the same lineage. The resolved topologies with maximum parsimony and Bayesian approaches clustered the specimens of O. hubbsorum and O. mimus in a single clade with high bootstrap and posterior probability values (100 and 1.0, respectively). These results allow us to suggest that O. hubbsorum and O. mimus could represent the same species. Source

Builes J.J.,Laboratorio Genes Ltda | Builes J.J.,University of Antioquia | Aguirre D.,Laboratorio Genes Ltda | Manrique A.,Laboratorio Genes Ltda | And 31 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

Colombian Reference National Laboratory, GENES LTDA, have organized and coordinated for the past two years (2009 and 2010) the Quality Control Exercise for laboratories undertaking paternity, maternity and forensic tests with DNA markers. Twenty-two laboratories have participated in 2009, increasing the number to 27 in 2010. Laboratories in Colombia, Brazil, Ecuador, Peru, Dominican Republic and Panama have participated in these exercises. There have been some similarities in the two controls: A practical exercise, three blood samples on FTA cards were sent to each participating laboratory to be genotyped for DNA markers using the routine methodologies in their laboratories; theoretical exercises including optional and obligatory cases. For the theoretical exercises, the participating laboratories should calculate the partial and final PI or BRI (Biological Relationship Index or Paternity Index). Forty-nine and 52 markers were under consensus for 2009 and 2010, respectively, distributed in autosomal, Y and X chromosomes STR. With respect to 2008, 12 and 15 additional markers were under consensus for 2009 and 2010, respectively. The rate of reporting error was 2.9% in 2009 while in 2010, 4.7% error was reported. The Proficiency Test conducted through the Colombian National Reference Laboratory has become a useful tool for quality assurance of all Colombian laboratories and some of Latin America that do DNA testing to establish biological relationships and an excellent opportunity for ongoing training of experts from the region. © 2011 Elsevier Ireland Ltd. Source

de los Barriga-Sosa I.A.,Laboratorio Of Genetica Y Biologia Molecular | Rojas-Esquivel R.,Laboratorio Of Genetica Y Biologia Molecular | Roldan M.I.,University of Girona
Reviews in Fish Biology and Fisheries | Year: 2011

The taxonomic status of hake (Merluccius spp.) in the northeastern Pacific is unclear. Hakes in this region are Merluccius productus, M. angustimanus, M. hernandezi, and a morphotype known as dwarf hake. Of these, only the first two species are currently valid. Descriptions in previous studies have been limited by overlapping morphological characteristic, lack of biological material, and limited numbers of sampling localities. To clarify their taxonomy, 461 hake were obtained from eight localities along the North American coast for morphological and mitochondrial DNA sequence analyses (cytochrome b, cytochrome c oxidase subunit I, and 16S ribosomal rDNA). Morphological and molecular analyses suggest that hake in this region represent a continuum of a single species with some levels of morphological and genetic intra-specific variation. In light of these results, we propose that M. productus is the only species of hake present along the North American and northern Central American coast. © 2010 Springer Science+Business Media B.V. Source

Hernandez-Romano P.,Centro Estatal Of La Transfusion Sanguinea Del Estado Of Veracruz | Camara-Contreras M.,Laboratorio Of Genetica Y Biologia Molecular | Bravo-Sarmiento E.,Centro Estatal Of La Transfusion Sanguinea Del Estado Of Veracruz | Lopez-Balderas N.,Laboratorio Of Genetica Y Biologia Molecular
Transfusion | Year: 2015

Background Trypanosoma cruzi is the causal agent of Chagas disease. Of the Mexican states, Veracruz is among the most affected by this sickness. However, the actual epidemiologic situation of this disease is not well understood. This study sought to determine the prevalence and risk factors for Chagas disease among Veracruzan blood donors. Study Design and Methods Blood donors from Centro Estatal de la Transfusion Sanguinea de Veracruz were included. Blood units were serologically scrutinized for T.-cruzi antibodies using enzyme-linked immunosorbent assays. To identify risk factors, demographic data were collected from the medical records of positive donors and a representative sample of healthy donors. Results A total of 87,232 donations were analyzed, and the mean prevalence of T.-cruzi was found to be 0.5%. The identified risk factors were living as a couple and in a rural area, having a low level of education, being a farmer, dwelling in a house with earthen or wooden walls and a tile or thatch roof, living with domestic animals, recognition of or exposure to triatomine bugs, and residing in the Huasteca region. An increase of rural-living donors infected with T.-cruzi was observed in the past 3 years of the study period. Conclusion The prevalence to Chagas disease has not decreased in the past decade and the disease appears to be spreading in rural areas of Veracruz. This increases the risk of T.-cruzi transfusion-transmitted infection, not only in Veracruz and Mexico, but also in other nonendemic countries that receive immigrants from Veracruz State. © 2014 AABB. Source

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