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Santa Tecla, El Salvador

Francez P.A.C.,Laboratorio Of Genetica Forense | Francez P.A.C.,Federal University of Para | Ramos L.P.V.,Faculdade SEAMA | Palha T.J.B.F.,Federal University of Para | dos Santos S.E.B.,Federal University of Para
Genetics and Molecular Biology | Year: 2012

The allelic and haplotype frequencies of 17 Y-STR loci most commonly used in forensic testing were estimated in a ample of 138 unrelated healthy males from Macapá, in the northern Amazon region of Brazil. The average gene diversity as 0.6554 ± 0.3315. 134 haplotypes of the 17 loci were observed, 130 of them unique and four present in two ndividuals each. The haplotype diversity index was 0.9996 ± 0.0009, with the most frequent haplogroups being R1b 52.2%), E1b1b (11.6%), J2 (10.1%) and Q (7.2%). Most haplogroups of this population belonged to European male ineages (89.2%), followed by Amerindian (7.2%) and African (3.6%) lineages. © 2012, Sociedade Brasileira de Genética. Source


Francez P.A.D.C.,Laboratorio Of Genetica Forense | Francez P.A.D.C.,Federal University of Para | Ribeiro-Rodrigues E.M.,Federal University of Para | Dos Santos S.E.B.,Federal University of Para
Forensic Science International: Genetics | Year: 2012

Allelic frequencies of 48 informative insert-delete (INDEL) loci were obtained from a sample set of 130 unrelated individuals living in Macapá, a city located in the northern Amazon region, in Brazil. The values of heterozygosity (H), polymorphic information content (PIC), power of discrimination (PD), power of exclusion (PE), matching probability (MP) and typical paternity index (TPI) were calculated and showed the forensic efficiency of these genetic markers. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 50%, 21% and 29%. Comparing these allele frequencies with those of other Brazilian populations and the parental populations, statistically significant distances were found. The interpopulation genetic distance (F ST coefficients) to the present database ranged from F ST = 0.0431 (p < 0.00001) between Macapá and Belém to F ST = 0.266 (p < 0.00001) between Macapá and the Native American group. © 2011 Elsevier Ireland Ltd. All rights reserved. Source


Da Costa Francez P.A.,Laboratorio Of Genetica Forense | Da Costa Francez P.A.,Federal University of Para | Rodrigues E.M.R.,Federal University of Para | De Velasco A.M.,Laboratorio Of Antropologia | Dos Santos S.E.B.,Federal University of Para
International Journal of Legal Medicine | Year: 2012

Insertion-deletion (INDEL) markers are very frequent in the human genome and present several advantages for population and forensic studies, such as low mutation rates, easy interpretation, small amplicons, easy genotyping, and the possibility of using multiplex PCR. The great adaptability of INDELs for amplification of low copy number or degraded DNA allows its using as an interesting platform of genetic identity by DNA in forensic cases. In the present study, we tested the ability of 48 diallelic INDEL markers on genotyping forensic samples collected from different biological samples related to criminal cases. Moreover, we evaluated the lowest DNA concentration with which there was amplification of all markers from each one of three indel-plex panels. When comparing the performances obtained by the indel-plex panels described in this study with results obtained using Identifiler® kit (Applied Biosystems) related to forensic samples, as well as to control samples with different concentrations of DNA, we observed superior efficiency on samples with low copy number or in the presence of inhibitors. © 2012 Springer-Verlag. Source


Juarez-Velazquez R.,Instituto Mexicano del Seguro Social | Juarez-Velazquez R.,Instituto Nacional Of Pediatria | Canto P.,Institute Seguridad Y Servicios Sociales Of Los Trabajadores Del Estado | Rangel-Villalobos H.,University of Guadalajara | And 8 more authors.
Disease Markers | Year: 2010

Several polymorphisms related to hypertension, thrombophilia, and oxidative stress has been associated with the development of cardiovascular disease. We analyzed the frequency of M235T angiotensinogen (AGT), A222V 5,10 methylenete-trahydrofolate reductase (MTHFR), L33P glycoprotein IIIa (GPIIIa), and I105V glutathione S-transferase P1 (GSTP1)} polymorphisms in 285 individuals belonging to Mexican-Mestizo and five Amerindian population from México, by real time PCR allelic discrimination. Allele and genotype frequencies were compared using χ2 tests. All populations followed the Hardy Weinberg equilibrium for assay markers with the exception of the Triki, whose were in Hardy Weinberg dysequilibrium for the glutathione S-transferase P1 polymorphism. Interestingly, according to all the analyzed single nucleotide polymorphisms (SNPs), the Triki population was the most differentiated and homogeneous group of the six populations analyzed. A comparison of our data with those previously published for some Caucasian, Asian and Black populations showed quite significant differences. These differences were remarkable with all the Mexican populations having a lower frequency of the 105V allele of the glutathione S-transferase P1 and reduced occurrence of the 222A allele of the 5,10 methylenetetrahydrofolate reductase. Our results show the genetic diversity among different Mexican populations and with other racial groups. © 2010 - IOS Press and the authors. All rights reserved. Source


da Francez P.A.C.,Laboratorio Of Genetica Forense | da Francez P.A.C.,Federal University of Para | Rodrigues E.M.R.,Federal University of Para | Frazao G.F.,Faculdade SEAMA | And 2 more authors.
Genetics and Molecular Biology | Year: 2011

The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (FST coefficients) to the present database ranged from FST = 0.0016 between Macapá and Belém to FST = 0.0036 between Macapá and the Iberian Peninsula. © 2011, Sociedade Brasileira de Genética. Source

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