Genova, Italy
Genova, Italy

Time filter

Source Type

PubMed | Laboratorio Of Genetica, University of Campinas, University of Sao Paulo and University of Naples Federico II
Type: | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2016

Cancer is a group of highly complex and heterogeneous diseases with several causes. According to the stochastic model, cancer initiates from mutation in somatic cells, leading to genomic instability and cell transformation. This canonical pathway of carcinogenesis is related to the discovery of important mechanisms that regulate cancer initiation. However, there are few studies describing genetic and metabolic alterations that deregulate transformed cells, resulting in epithelial-mesenchymal transition (EMT) and its most dramatic consequence, the metastasis. This review summarizes the main genetics and metabolic changes induced by reactive oxygen species (ROS) that lead to EMT.


PubMed | Hospital Infantil Federico Gomez and Laboratorio Of Genetica
Type: Journal Article | Journal: Gaceta medica de Mexico | Year: 2016

To estimate the evaluation and intervention thresholds using FRAX in Mexican population.Probabilities for a mayor fracture using the Mexican FRAX version to estimate the evaluation and intervention thresholds using clinical scenarios were obtained in both sexes 40 years and over. Projections for 2020 were done to estimate the number of patients at the intervention level taking the osteoporosis prevalence in Mexicans aged 50 years and over.The cutoffs for the intervention thresholds were 2.6%-20.0%. Individuals with thresholds above these probabilities are amenable for intervention. The assessment thresholds range from 1.2-3.2% to 12.5-24.4% for 40 to 90 years. According to projections of our population, approximately three million people are susceptible to intervention by 2020.The use of FRAX thresholds of intervention and evaluation developed in this study will be useful in the primary care level for case detection at high risk of fragility fracture.


Piccione M.,University of Palermo | Piro E.,University of Palermo | Serraino F.,University of Palermo | Cavani S.,Laboratorio Of Genetica | And 6 more authors.
European Journal of Medical Genetics | Year: 2012

We report two individuals with developmental delay and dysmorphic features, in whom array-based comparative genomic hybridization (array CGH) led to the identification of a 2p15p16.1 . de novo deletion. In the first patient (Patient 1) a familial deletion of 6q12, inherited from her father, was also detected. In the second patient (Patient 2) in addition to the 2p15p16.1 microdeletion a . de novo deletion in Xq28 was detected. Both individuals shared dysmorphic features and developmental delay with the six reported patients with a 2p15p16.1 microdeletion described in medical literature. Conclusion: in the first patient a 642 kb 2p16.1 deletion (from 60.604 to 61.246 Mb), and a 930 kb 6q12 familial deletion, was detected and in the second a 2.5 Mb 2p15p16.1 deletion (from 60.258 to 62.763 Mb), with a Xq28 deletion, was discovered. The common dysmorphic features and neurodevelopmental delay found in these patients are in agreement with the clinical phenotype of a microdeletion syndrome involving 2p15p16.1. Our data confirm the hypothesis suggesting that 2p15p16.1 deletion is a contiguous gene syndrome. © 2012 Elsevier Masson SAS.


Alvarez-Gonzalez I.,Laboratorio Of Genetica | Mojica R.,Laboratorio Of Genetica | Madrigal-Bujaidar E.,Laboratorio Of Genetica | Camacho-Carranza R.,National Autonomous University of Mexico | And 2 more authors.
Food and Chemical Toxicology | Year: 2011

We determined the capacity of grapefruit juice (GJ) to inhibit the rate of micronucleated polychromatic erythrocytes (MNPE) in mice treated with benzo(a)pyrene (BaP), an environmental contaminant that is biotransformed by Cyp1a1 and is a strong genotoxic agent. For this study, we administered 4.1, 20.8, and 41.6. μl/g body weight (b.w.) of GJ to BaP-treated mice (340 mg/kg). We found a significant decrease in the frequency of MNPE at 48 and 72 h compared to BaP-only treated animals. In turn, no prevention of the cytotoxic damage induced by BaP was found. We next explored whether GJ's antigenotoxic mechanism of action was related to an inhibitory effect on the activity of the Cyp1a1 enzyme. A reduction in microsomal hepatic and intestinal ethoxyresorufin-O-deethylase (EROD) activity of 20% and 44%, respectively, was found in mice treated with BaP and GJ compared to BaP-only treated animals. Furthermore, when EROD inhibition was tested in vitro, we found a concentration-dependent EROD inhibition by GJ, which reached 85% of the maximum level. Together, these results suggest that the protective effect of GJ against the genotoxicity of BaP may be related to the inhibition of Cyp1a1 enzyme activity. © 2010 Elsevier Ltd.


Garcia-Medina S.,Laboratorio Of Toxicologia Acuatica | Razo-Estrada C.,Laboratorio Of Toxicologia Acuatica | Galar-Martinez M.,Laboratorio Of Toxicologia Acuatica | Cortez-Barberena E.,Metropolitan Autonomous University | And 3 more authors.
Comparative Biochemistry and Physiology - C Toxicology and Pharmacology | Year: 2011

Few studies have been made in regard to the effect of aluminum on the molecular and cellular structure and function of aquatic organisms; therefore, in the present report we determined the genotoxic and cytotoxic effects induced by the metal on the lymphocytes of carp (Cyprinus carpio). Three groups of fish were exposed to 0.05, 120, and 239 mg/L of aluminum (Al), respectively, by using Al2 (SO4)3•7H2O, and another group was included as control. The cells obtained were studied with the comet assay, flow cytometry, and the TUNEL method. With the first method we found a concentration and time dependent, significant increase in the amount of DNA damage induced by Al, and a higher damage when we evaluated the level of oxidized DNA. By applying flow cytometry we established that the metal induced a DNA content increase and ploidy modifications as well as apoptosis and disturbances of the cell cycle progression. With the last method we determined a significant increase in the amount of apoptotic cells, mainly in the 72-96 h period. Our results established that Al caused deleterious DNA and cellular effects in the tested organism, and they suggested the pertinence of evaluating toxicity induced by the metal in organisms living in contaminated water bodies. © 2010 Elsevier Inc.


Teixeira D.H.L.,Federal University of Lavras | de Oliveira M.S.P.,Laboratorio Of Genetica | Goncalves F.M.A.,Federal University of Lavras | Nunes J.A.R.,Federal University of Lavras
Revista Brasileira de Fruticultura | Year: 2012

The knowledge of the genetics correlations among the main characters used in plant breeding helps to elaborate optimized strategies of selection. The procedure of path analysis allows refining these correlations and partitioning them into direct and indirect effects on a main variable. The objective of this study was to partition the genetic correlations of traits concerning to bunch and yield of half-sib progenies into direct and indirect effects on total fruit production. It was installed an experiment with 25 half-sib progenies in random blocks with four repetitions and linear plots of five plants. The data obtained over the crop years 2005, 2006 and 2007 was analyzed by mixed models approach and genetics correlations submitted to path analysis. The fruits production showed correlation with number of months in production, total number of bunch, fruit weight per bunch and number of rachilles per bunch. However, by path analysis, the main determinants characters of variation on fruit production of assai palm were fruits weight per bunch, number of bunches and number of rachilles per bunch. Among these characters, the number of rachilles per bunch is less affected by the environment, and therefore, is the most promising for indirect gains in total fruit production.


Garcia-Cegarra A.,Laboratorio Of Genetica | Merlo M.A.,Laboratorio Of Genetica | Ponce M.,Laboratorio Of Biologia Molecular | Portela-Bens S.,Laboratorio Of Genetica | And 3 more authors.
Cytogenetic and Genome Research | Year: 2013

This article presents the first physical mapping carried out in the Senegalese sole (Solea senegalensis), an important marine fish species of Southern Europe. Eight probes were designated to pick up genes of interest in aquaculture (candidate genes) from a bacterial artificial chromosome (BAC) library using a method of rapid screening based on a 4-dimension PCR. Seven known and 3 unknown clones were isolated and labeled. The 10 BAC clones were used as probes to map the karyotype of the species by fluorescence in situ hybridization (FISH). Nine out of the 10 clones were localized in only 1 chromosome pair, whereas the remaining one hybridized on 2 chromosome pairs. The 2-color FISH experiments showed colocation of 4 probes in 2 chromosome pairs. In addition, 2-color FISH was carried out both with 5S rDNA and the BAC containing the lysozyme gene published previously. This first genetic map of the Senegalese sole represents a starting point for future studies of the sole genome. In addition, 7 out of the 10 BAC clones were sequenced using next-generation sequencing, and bioinformatic characterization of the sequences was carried out. Hence the anchoring of the sequences to specific chromosomes or chromosome arms is now possible, leading to an initial scaffold of the Senegalese sole genome. © 2013 S. Karger AG, Basel.


Madrigal-Bujaidar E.,Laboratorio Of Genetica | Cardenas Garcia Y.,Laboratorio Of Genetica | Alvarez-Gonzalez I.,Laboratorio Of Genetica
Human and Experimental Toxicology | Year: 2010

Imipramine (IMI) and desipramine (DES) are two drugs widely used for the treatment of depression as well as for other diseases. In the present study, we determined their capacity to induce chromosomal aberrations in mouse bone marrow cells. Three doses of each compound were tested and their results were compared with the frequency of chromosomal aberrations obtained in a control group as well as with a group treated with cyclophosphamide. Our results showed a significant increase in chromosome damage with the doses tested for each compound: 7, 20, and 60 mg/kg in the case of IMI, and 2, 20, and 60 mg/kg as regards DES. This last drug induced stronger chromosomal damage than IMI. Our results agree with previous studies regarding the induction of micronuclei and sister chromatid exchanges by the drugs in mouse and suggest caution with respect to their use in long-term treatments.


Alvarez-Gonzalez I.,Laboratorio Of Genetica | Madrigal-Bujaidar E.,Laboratorio Of Genetica | Sanchez-Garcia V.Y.,Laboratorio Of Genetica
Plant Foods for Human Nutrition | Year: 2010

The consumption of grapefruit juice (GJ) has been associated with various activities potentially beneficial for human health, including protection against the DNA damage produced by various compounds. In the present report, we evaluated the capability of this juice to inhibit the rate of micronucleated polychromatic erythrocytes (MNPE) and sister chromatid exchanges (SCE) induced by the antineoplastic alkylating agent ifosfamide (IF). We tested the effect of administering 100, 500, and 1000 mg/kg of GJ in mouse and obtained the following results: a) with the high dose, a reduction of 72% in the rate of SCE induced by IF; b) a mean reduction of 65.3% in the rate of MNPE compared with the damage induced by IF at 48 h post-administration; c) no modification induced by GJ either on the cellular proliferation kinetics or in the mitotic index; and d) neither induction of bone marrow cytotoxicity by GJ nor a protective effect of the juice against the cytotoxicity induced by IF. These data showed a significant inhibitory effect of GJ against the chromosome damage induced by the tested agent; they also suggest the relevance of carrying out studies to clarify the involved mechanism of action as well as to determine the cancer chemopreventive capacity of the juice. © 2010 Springer Science+Business Media, LLC.


PubMed | Laboratorio Of Genetica
Type: Journal Article | Journal: World journal of gastrointestinal pharmacology and therapeutics | Year: 2016

Helicobacter pylori (H. pylori) has long been found to cause gastric diseases such as gastritis, gastric ulcers and gastric cancer. The transmission medium of this bacterium has yet to be determined, though several studies have speculated that the oral cavity is a reservoir for H. pylori. Others have also reported that the oral cavity may be a source of both transmission and gastric reinfection; however, such results are controversial. We reviewed the literature and selected studies that report an association among H. pylori detections in the oral cavity (dental plaque, saliva, tongue, tonsil tissue, root canals, oral mucosa) in humans and in animals, as well as in the human stomach. The oral cavity may be considered the main reservoir for H. pylori. There are a correlations between H. pylori infection in the oral cavity and periodontal disease, oral tissue inflammation, H. pylori transmission, and gastric reinfection. We believe that the mouth is a reservoir and that it plays a crucial role in both H. pylori transmission and gastric infection.

Loading Laboratorio Of Genetica collaborators
Loading Laboratorio Of Genetica collaborators