Laboratorio Of Genetica

Genova, Italy

Laboratorio Of Genetica

Genova, Italy
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Furuya T.K.,Federal University of São Paulo | Silva P.N.O.,Federal University of São Paulo | Payao S.L.M.,Federal University of São Paulo | Payao S.L.M.,Laboratorio Of Genetica | And 10 more authors.
Neuroscience | Year: 2012

Alzheimer's Disease (AD) is the most common cause of dementia in elderly people. The presynaptic terminal is an important site of pathological changes in AD, leading to synaptic loss in specific brain regions, such as in the cortex and hippocampus. In this study, we investigated synaptosomal-associated protein, 25-kDa (SNAP25) mRNA levels and promoter DNA methylation in post mortem brain tissues (entorhinal and auditory cortices and hippocampus) from healthy elderly and AD subjects as well as in peripheral blood leukocytes of young, healthy elderly and AD patients. mRNA quantification was performed by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) using the ΔΔCT method and promoter DNA methylation was quantified by mass spectrometry using the Sequenom EpiTYPER platform. We observed a significant decrease in SNAP25 expression in AD across all the three brain regions in relation to the healthy elderly subjects, suggesting impairment in synaptic function. The changes in the auditory cortex reflected those observed in the hippocampus and entorhinal cortex, the primary areas affected in AD. However, no AD-associated differences in SNAP25 promoter DNA methylation were observed suggesting that other mechanisms may be involved in mediating the observed gene expression changes. © 2012 IBRO.


Carino-Cortes R.,Institute Ciencias Of La Salud | Carino-Cortes R.,Laboratorio Of Genetica | Alvarez-Gonzalez I.,Laboratorio Of Genetica | Martino-Roaro L.,Laboratorio Of Genetica | Madrigal-Bujaidar E.,Institute Ciencias Of La Salud
Biological and Pharmaceutical Bulletin | Year: 2010

Naringin (Nar) is a flavonoid that has shown antigenotoxic effect against the chromosome damage induced by various compounds. The aims of the present investigation on Nar were threefold: a) to determine its DNA breaking potential in mouse hepatocytes and cardiocytes, b) to evaluate its capacity to inhibit the DNA damage induced by daunorubicin (Dau) in the same tissues, and c) to determine its capacity to trap free radicals in vitro using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) method. For the two first purposes we applied the comet assay to three groups of animals administered with Nar by the oral route (50, 250, 500mg/kg), and made the observations before the chemical administration and at 3, 12, and 21h postadministration. Other three groups of mice were given equal doses of Nar, and 1h later they were intraperitoneally injected with 1mg/kg of Dau. The results showed that Nar did not induce DNA breakage in both types of studied cells, in contrast with the significant damage induced by Dau in hepatocytes and cardiocytes. Moreover, the administration of Nar protected the DNA damage produced by Dau, showing a maximum reduction of 71.3% and 51.1% in hepatocytes and cardiocytes, respectively. With respect to the antioxidant potential, 20mM of Nar produced a free radical scavenging activity as high as 95%. Our study established a high DNA breaking potential of Dau, and a protective effect by Nar, probably related with its capacity to trap free radicals. © 2010 Pharmaceutical Society of Japan.


Araldi R.P.,Laboratorio Of Genetica | Araldi R.P.,University of Sao Paulo | Modolo D.G.,Laboratorio Of Genetica | de Sa Junior P.L.,Laboratorio Of Genetica | And 5 more authors.
Biomedicine and Pharmacotherapy | Year: 2016

Cancer is a group of highly complex and heterogeneous diseases with several causes. According to the stochastic model, cancer initiates from mutation in somatic cells, leading to genomic instability and cell transformation. This canonical pathway of carcinogenesis is related to the discovery of important mechanisms that regulate cancer initiation. However, there are few studies describing genetic and metabolic alterations that deregulate transformed cells, resulting in epithelial-mesenchymal transition (EMT) and its most dramatic consequence, the metastasis. This review summarizes the main genetics and metabolic changes induced by reactive oxygen species (ROS) that lead to EMT. © 2016 Elsevier Masson SAS.


PubMed | Laboratorio Of Genetica, University of Campinas, University of Sao Paulo and University of Naples Federico II
Type: | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2016

Cancer is a group of highly complex and heterogeneous diseases with several causes. According to the stochastic model, cancer initiates from mutation in somatic cells, leading to genomic instability and cell transformation. This canonical pathway of carcinogenesis is related to the discovery of important mechanisms that regulate cancer initiation. However, there are few studies describing genetic and metabolic alterations that deregulate transformed cells, resulting in epithelial-mesenchymal transition (EMT) and its most dramatic consequence, the metastasis. This review summarizes the main genetics and metabolic changes induced by reactive oxygen species (ROS) that lead to EMT.


Piccione M.,University of Palermo | Piro E.,University of Palermo | Serraino F.,University of Palermo | Cavani S.,Laboratorio Of Genetica | And 6 more authors.
European Journal of Medical Genetics | Year: 2012

We report two individuals with developmental delay and dysmorphic features, in whom array-based comparative genomic hybridization (array CGH) led to the identification of a 2p15p16.1 . de novo deletion. In the first patient (Patient 1) a familial deletion of 6q12, inherited from her father, was also detected. In the second patient (Patient 2) in addition to the 2p15p16.1 microdeletion a . de novo deletion in Xq28 was detected. Both individuals shared dysmorphic features and developmental delay with the six reported patients with a 2p15p16.1 microdeletion described in medical literature. Conclusion: in the first patient a 642 kb 2p16.1 deletion (from 60.604 to 61.246 Mb), and a 930 kb 6q12 familial deletion, was detected and in the second a 2.5 Mb 2p15p16.1 deletion (from 60.258 to 62.763 Mb), with a Xq28 deletion, was discovered. The common dysmorphic features and neurodevelopmental delay found in these patients are in agreement with the clinical phenotype of a microdeletion syndrome involving 2p15p16.1. Our data confirm the hypothesis suggesting that 2p15p16.1 deletion is a contiguous gene syndrome. © 2012 Elsevier Masson SAS.


Alvarez-Gonzalez I.,Laboratorio Of Genetica | Mojica R.,Laboratorio Of Genetica | Madrigal-Bujaidar E.,Laboratorio Of Genetica | Camacho-Carranza R.,National Autonomous University of Mexico | And 2 more authors.
Food and Chemical Toxicology | Year: 2011

We determined the capacity of grapefruit juice (GJ) to inhibit the rate of micronucleated polychromatic erythrocytes (MNPE) in mice treated with benzo(a)pyrene (BaP), an environmental contaminant that is biotransformed by Cyp1a1 and is a strong genotoxic agent. For this study, we administered 4.1, 20.8, and 41.6. μl/g body weight (b.w.) of GJ to BaP-treated mice (340 mg/kg). We found a significant decrease in the frequency of MNPE at 48 and 72 h compared to BaP-only treated animals. In turn, no prevention of the cytotoxic damage induced by BaP was found. We next explored whether GJ's antigenotoxic mechanism of action was related to an inhibitory effect on the activity of the Cyp1a1 enzyme. A reduction in microsomal hepatic and intestinal ethoxyresorufin-O-deethylase (EROD) activity of 20% and 44%, respectively, was found in mice treated with BaP and GJ compared to BaP-only treated animals. Furthermore, when EROD inhibition was tested in vitro, we found a concentration-dependent EROD inhibition by GJ, which reached 85% of the maximum level. Together, these results suggest that the protective effect of GJ against the genotoxicity of BaP may be related to the inhibition of Cyp1a1 enzyme activity. © 2010 Elsevier Ltd.


Teixeira D.H.L.,Federal University of Lavras | de Oliveira M.S.P.,Laboratorio Of Genetica | Goncalves F.M.A.,Federal University of Lavras | Nunes J.A.R.,Federal University of Lavras
Revista Brasileira de Fruticultura | Year: 2012

The knowledge of the genetics correlations among the main characters used in plant breeding helps to elaborate optimized strategies of selection. The procedure of path analysis allows refining these correlations and partitioning them into direct and indirect effects on a main variable. The objective of this study was to partition the genetic correlations of traits concerning to bunch and yield of half-sib progenies into direct and indirect effects on total fruit production. It was installed an experiment with 25 half-sib progenies in random blocks with four repetitions and linear plots of five plants. The data obtained over the crop years 2005, 2006 and 2007 was analyzed by mixed models approach and genetics correlations submitted to path analysis. The fruits production showed correlation with number of months in production, total number of bunch, fruit weight per bunch and number of rachilles per bunch. However, by path analysis, the main determinants characters of variation on fruit production of assai palm were fruits weight per bunch, number of bunches and number of rachilles per bunch. Among these characters, the number of rachilles per bunch is less affected by the environment, and therefore, is the most promising for indirect gains in total fruit production.


Garcia-Cegarra A.,Laboratorio Of Genetica | Merlo M.A.,Laboratorio Of Genetica | Ponce M.,Laboratorio Of Biologia Molecular | Portela-Bens S.,Laboratorio Of Genetica | And 3 more authors.
Cytogenetic and Genome Research | Year: 2013

This article presents the first physical mapping carried out in the Senegalese sole (Solea senegalensis), an important marine fish species of Southern Europe. Eight probes were designated to pick up genes of interest in aquaculture (candidate genes) from a bacterial artificial chromosome (BAC) library using a method of rapid screening based on a 4-dimension PCR. Seven known and 3 unknown clones were isolated and labeled. The 10 BAC clones were used as probes to map the karyotype of the species by fluorescence in situ hybridization (FISH). Nine out of the 10 clones were localized in only 1 chromosome pair, whereas the remaining one hybridized on 2 chromosome pairs. The 2-color FISH experiments showed colocation of 4 probes in 2 chromosome pairs. In addition, 2-color FISH was carried out both with 5S rDNA and the BAC containing the lysozyme gene published previously. This first genetic map of the Senegalese sole represents a starting point for future studies of the sole genome. In addition, 7 out of the 10 BAC clones were sequenced using next-generation sequencing, and bioinformatic characterization of the sequences was carried out. Hence the anchoring of the sequences to specific chromosomes or chromosome arms is now possible, leading to an initial scaffold of the Senegalese sole genome. © 2013 S. Karger AG, Basel.


Madrigal-Bujaidar E.,Laboratorio Of Genetica | Cardenas Garcia Y.,Laboratorio Of Genetica | Alvarez-Gonzalez I.,Laboratorio Of Genetica
Human and Experimental Toxicology | Year: 2010

Imipramine (IMI) and desipramine (DES) are two drugs widely used for the treatment of depression as well as for other diseases. In the present study, we determined their capacity to induce chromosomal aberrations in mouse bone marrow cells. Three doses of each compound were tested and their results were compared with the frequency of chromosomal aberrations obtained in a control group as well as with a group treated with cyclophosphamide. Our results showed a significant increase in chromosome damage with the doses tested for each compound: 7, 20, and 60 mg/kg in the case of IMI, and 2, 20, and 60 mg/kg as regards DES. This last drug induced stronger chromosomal damage than IMI. Our results agree with previous studies regarding the induction of micronuclei and sister chromatid exchanges by the drugs in mouse and suggest caution with respect to their use in long-term treatments.


Alvarez-Gonzalez I.,Laboratorio Of Genetica | Madrigal-Bujaidar E.,Laboratorio Of Genetica | Sanchez-Garcia V.Y.,Laboratorio Of Genetica
Plant Foods for Human Nutrition | Year: 2010

The consumption of grapefruit juice (GJ) has been associated with various activities potentially beneficial for human health, including protection against the DNA damage produced by various compounds. In the present report, we evaluated the capability of this juice to inhibit the rate of micronucleated polychromatic erythrocytes (MNPE) and sister chromatid exchanges (SCE) induced by the antineoplastic alkylating agent ifosfamide (IF). We tested the effect of administering 100, 500, and 1000 mg/kg of GJ in mouse and obtained the following results: a) with the high dose, a reduction of 72% in the rate of SCE induced by IF; b) a mean reduction of 65.3% in the rate of MNPE compared with the damage induced by IF at 48 h post-administration; c) no modification induced by GJ either on the cellular proliferation kinetics or in the mitotic index; and d) neither induction of bone marrow cytotoxicity by GJ nor a protective effect of the juice against the cytotoxicity induced by IF. These data showed a significant inhibitory effect of GJ against the chromosome damage induced by the tested agent; they also suggest the relevance of carrying out studies to clarify the involved mechanism of action as well as to determine the cancer chemopreventive capacity of the juice. © 2010 Springer Science+Business Media, LLC.

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