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Silva L.P.,Laboratorio Of Espectrometria Of Massa
Animal Reproduction | Year: 2014

This review focus on the potential benefits, threats, and challenges of nanotechnology in animal reproduction. The investigation of gamete cells in high-resolution, production of nanobiosensors, and development of nanosystems aiming the sustained release of gonadotropins and steroid hormones are only some few examples of growing interest areas. Current facts and future prospects have highlighted the great potential of nanotechnology in reproduction field. Emerging concepts and technologies will be contextualized, reviewed, and explored in this review.

Ramada M.H.S.,University of Brasilia | Ramada M.H.S.,Laboratorio Of Espectrometria Of Massa | Steindorff A.S.,University of Brasilia | Bloch C.,Laboratorio Of Espectrometria Of Massa | Ulhoa C.J.,Federal University of Goais
Proteomics | Year: 2016

Trichoderma harzianum is a fungus well known for its potential as a biocontrol agent against many fungal phytopathogens. The aim of this study was to characterize the proteins secreted by T. harzianum ALL42 when its spores were inoculated and incubated for 48 h in culture media supplemented with glucose (GLU) or with cell walls from Fusarium solani (FSCW), a phytopathogen that causes severe losses in common bean and soy crops in Brazil, as well as other crop diseases around the world. Trichoderma harzianum was able to grow in Trichoderma Liquid Enzyme Production medium (TLE) and Minimal medium (MM) supplemented with FSCW and in TLE+GLU, but was unable to grow in MM+GLU medium. Protein quantification showed that TLE+FSCW and MM+FSCW had 45- and 30- fold, respectively, higher protein concentration on supernatant when compared to TLE+GLU, and this difference was observable on 2D gel electrophoresis (2DE). A total of 94 out of 105 proteins excised from 2DE maps were identified. The only protein observed in all three conditions was epl1. In the media supplemented with FSCW, different hydrolases such as chitinases, β-1,3-glucanases, glucoamylases, α-1,3-glucanases and proteases were identified, along with other proteins with no known functions in mycoparasitism, such as npp1 and cys. Trichoderma harzianum showed a complex and diverse arsenal of proteins that are secreted in response to the presence of FSCW, with novel proteins not previously described in mycoparasitic-related studies. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Barbosa E.F.,Laboratorio Of Espectrometria Of Massa | Barbosa E.F.,University of Brasilia | Silva L.P.,Laboratorio Of Espectrometria Of Massa | Silva L.P.,University of Brasilia
Journal of Membrane Science | Year: 2012

Synthetic polymeric porous membranes (PPMs) have been widely used in several areas. Here, PPMs were scrutinized in a systematic approach over their mechanostructural features, chemical composition and filtration efficiency. Four types of PPMs (nitrocellulose, nylon, PTFE, and PVDF) were analyzed by single point force spectroscopy (FS), atomic force microscopy (AFM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and energy-dispersive X-ray spectroscopy (EDS). PTFE membrane showed discrepancy between top and bottom parts related to FS parameters. The most irregular topography assessed by AFM was presented by nylon membrane showing out values for roughness parameters (Ra, Rz, and Rp) higher that all other membranes. Determination of filtration efficiency by spectrophotometric measurements did not allow us to visualize tendencies for incrustation of compounds. Nevertheless, AFM and SEM images of PPMs before and after filtration tests showed significant topographical differences. FTIR and EDS analyses also evidenced PPMs chemical and elemental composition, respectively. The use of these approaches focusing on nanometric aspects of each membrane can reveal new possibilities concerning their characterization and applications. It arises as an alternative tool for quality control evaluation, or search for support for biomolecules immobilization. © 2012 Elsevier B.V.

Joanitti G.A.,Laboratorio Of Espectrometria Of Massa | Joanitti G.A.,University of Brasilia | Silva L.P.,Laboratorio Of Espectrometria Of Massa | Silva L.P.,University of Brasilia
Current Drug Targets | Year: 2014

Natural resources are widely used as raw materials by industries. In most cases, abundant byproducts with low economic interest are also generated from agro-industrial supply chains. There are several examples for the rational use of agro-industrial byproducts in the nanobiotechnology field aiming for the development of novel products and high value added processes. Such raw materials include carapaces, pelages, blood, bagasses, and straws. Molecules from such materials (e.g. chitosan, cellulose, and albumin) are used as scaffolds of unprecedented novel nanostructure. Research efforts comprising a combination of sustainability, nanobiotechnology, and nanomedicine have emerged. One major area in nano-biotechnological research of agro-industrial byproducts is represented by the field of drug delivery systems (DDS). Among the main advantages of agro-industrial byproducts used as drug carriers are their abundance; low price; high bio-compatibility; good biodegradability; moderate bioresorbability, associated with reduced systemic toxicity or even no tox-icity; and often bioactivity. The goal of these efforts includes not only the possibility to characterize and manipulate matter on the nanoscale, but also to develop sustainable products and processes, including the development of platforms for drug delivery aiming for the treatment of pathologies such as cancer and diabetes. Indeed, there is great hope that the use of agro-industrial byproducts in nanobiotechnology will increase not only agricultural and livestock productivity, but will also contribute to other areas such as the development of DDS with new properties and low production costs; and sustainable environmental management due to the reuse of industrial discharged byproducts. This review will compile current findings on the use of byproducts as building blocks for modern drug carrier systems, emphasizing the challenges and promising applications. © 2014 Bentham Science Publishers.

Barbosa E.F.,Laboratorio Of Espectrometria Of Massa | Barbosa E.F.,University of Brasilia | Silva L.P.,Laboratorio Of Espectrometria Of Massa | Silva L.P.,University of Brasilia
Surface and Interface Analysis | Year: 2013

A nanoscale characterization of modified and unmodified polypropylene (PP) microtubes internal surface was performed to investigate their structural, chemical, and physical properties. Nanoroughness, stiffness, elasticity, attraction behavior, adhesion forces, and chemical environment were investigated to test some manufacturer statements regarding Axygen MAXYMum Recovery® products. They announced that this class of material presented special features, originated from a modification to the original PP resin and by using a diamond polished mould, providing lower retention and minor interference on laboratorial tests, such as low roughness and little interaction tendency. Then, in this study, modified and control internal surfaces of PP microtubes were compared by atomic force microscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy. Nanoroughness and force spectroscopy parameters assessed by atomic force microscopy showed out as a sensible and high-resolution technique, crucial to discriminate differences between the surfaces. This type of investigation can be considered as a promising approach that can be applied to other polymeric systems, considering nanoscale properties, physical/chemical modifications, and as an alternative route for quality control checking concerning polymeric surfaces. © 2013 John Wiley & Sons, Ltd.

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