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Braga M.V.,Laboratorio Of Entomologia Medica E Forense | Mendonca P.M.,Laboratorio Of Entomologia Medica E Forense | Barbosa R.R.,Laboratorio Of Entomologia Medica E Forense | Blomquist G.J.,University of Nevada, Reno | And 5 more authors.
Journal of Natural History | Year: 2015

Insect puparia were found adhered to the ribs and other tissues in the abdominal cavity of a natural male mummy found in Itacambira (State of Minas Gerais, Brazil) dating to the Colonial Period. They were collected for identification by scanning electron microscopy, and for comparison of several morphological features with those described in the literature. The puparia were found open and dorsoventrally flattened, making it difficult to visualize the dorsal projections. The tegument is covered by tapered spines and contains rows of small tubercles on the dorsal and lateral regions of the puparium. The posterior spiracle consists of four parallel openings arranged in pairs. These results are indicative that the specimens belong to the species Megaselia scalaris (Loew, 1866) (Diptera: Phoridae). Additionally, cuticular hydrocarbons of the puparia were analysed by gas chromatography coupled to mass spectrometry and compared with the profile of M. scalaris reared in the laboratory. © 2015 Taylor & Francis

Carramaschi I.N.,Laboratorio Of Entomologia Medica E Forense | Pereira L.A.,Laboratorio Of Entomologia Medica E Forense | Queiroz M.M.C.,Laboratorio Of Entomologia Medica E Forense | Zahner V.,Laboratorio Of Entomologia Medica E Forense
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2015

Introduction: This study evaluated whether different strains of Brevibacillus laterosporus could be used to control larvae of the blowfly Chrysomya megacephala, a pest that affects both human and animal health. Methods: Mortality rates were recorded after 1-mL suspensions of sporulated cells of 14 different strains of B. laterosporus were added to 2.5g of premixed diet consisting of rotting ground beef fed to first instar larvae of C. megacephala. All bioassays were performed using 10 larvae per strain, with a minimum of three replicates for each bioassay. Larval mortality was recorded daily up to seven days. Results: Strains Bon 707, IGM 16-92, and Shi 3 showed the highest toxicity toward the larvae producing 70.5%, 64.5%, and 51.6% of larval mortality, respectively, which was significantly higher than that in the control group (p < 0.05). In contrast, strains NRS 1642, NRS 661, NRS 590 BL 856, NRS 342, ATCC 6457, Bon 712, and NRS 1247 showed limited or no pathogenic activity against the target larvae. Conclusions: Our preliminary data indicated that B. laterosporus could be used to develop bioinsecticides against C. megacephala. © 2015, Sociedade Brasileira de Medicina Tropical. All rights reserved.

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