Laboratorio Of Enterobacterias

Rio de Janeiro, Brazil

Laboratorio Of Enterobacterias

Rio de Janeiro, Brazil
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Dias M.K.R.,Federal University of Amapá | Sampaio L.S.,Federal University of Amapá | Proietti A.A.,Federal University of Amapá | Yoshioka E.T.O.,Federal University of Amapá | And 9 more authors.
Veterinary Microbiology | Year: 2016

Aeromonas hydrophila is causing substantial economic losses in world aquaculture. This study determined the tolerance limit (LD50-96h) of A. hydrophila in Arapaima gigas, and also investigated the clinical signs after intradermal inoculation. Arapaima gigas fingerlings were inoculated intraperitoneally with 0 (control), 1.0 × 105, 1.0 × 106, 1.0 × 107, 1.0 × 109 and 1.0 × 1010 CFU/mL of A. hydrophila for the determination of LD50-96h, which was 1.8 × 108 CFU/mL. In another trial with intradermal inoculation of 1.8 × 108 CFU/mL A. hydrophila, there was a 91.6% of mortality between 8 and 23 h, and several clinical signs were found. As follows: depigmentation in the tegument, lesions in the tail and fins, loss of balance, reduction of respiratory movements, hemorrhagic foci, necrotic hemorrhages in the kidney, liver and swim bladder, splenomegaly, ascites in the abdominal cavity and hyperemia, enlargement of the gall bladder, among other clinical signs observed. The results showed that A. gigas has a relative tolerance to A. hydrophila when compared to other Neotropical fish species. © 2016 Elsevier B.V.


PubMed | Abel Salazar Biomedical Sciences Institute, Laboratorio Of Enterobacterias, Federal University of Amapá and Federal University of Acre
Type: | Journal: Veterinary microbiology | Year: 2016

Aeromonas hydrophila is causing substantial economic losses in world aquaculture. This study determined the tolerance limit (LD50-96h) of A. hydrophila in Arapaima gigas, and also investigated the clinical signs after intradermal inoculation. Arapaima gigas fingerlings were inoculated intraperitoneally with 0 (control), 1.010(5), 1.010(6), 1.010(7), 1.010(9) and 1.010(10)CFU/mL of A. hydrophila for the determination of LD50-96h, which was 1.810(8)CFU/mL. In another trial with intradermal inoculation of 1.810(8)CFU/mL A. hydrophila, there was a 91.6% of mortality between 8 and 23h, and several clinical signs were found. As follows: depigmentation in the tegument, lesions in the tail and fins, loss of balance, reduction of respiratory movements, hemorrhagic foci, necrotic hemorrhages in the kidney, liver and swim bladder, splenomegaly, ascites in the abdominal cavity and hyperemia, enlargement of the gall bladder, among other clinical signs observed. The results showed that A. gigas has a relative tolerance to A. hydrophila when compared to other Neotropical fish species.


Morales A.S.,University of Sao Paulo | Fragoso De Araujo J.,University of Sao Paulo | De Moura Gomes V.T.,University of Sao Paulo | Reis Costa A.T.,Institute Pesquisas Veterinarias Especializadas IPEVE | And 5 more authors.
The Scientific World Journal | Year: 2012

Reports about acquired resistance to colistin in different bacteria species are increasing, including E. coli of animal origin, but reports of resistance in wild S. enterica of different serotypes from swine are not found in the literature. Results obtained with one hundred and twenty-six E. coli strains from diseased swine and one hundred and twenty-four S. enterica strains from diseased and carrier swine showed a frequency of 6.3 and 21 of colistin-resistant strains, respectively. When comparing the disk diffusion test with the agar dilution test to evaluate the strains, it was confirmed that the disk diffusion test is not recommended to evaluate colistin resistance as described previously. The colistin MIC 90 and MIC 50 values obtained to E. coli were 0.25 μg/mL and 0.5 μg/mL, the MIC 90 and MIC 50 to S. enterica were 1 μg/mL and 8 μg/mL. Considering the importance of colistin in control of nosocomial human infections with Gram-negative multiresistant bacteria, and the large use of this drug in animal production, the colistin resistance prevalence in enterobacteriaceae of animal origin must be monitored more closely. © 2012 Adriano Savoia Morales et al.


Lima A.L.,Federal Rural University of Rio de Janeiro | Rodrigues D.P.,Laboratorio Of Enterobacterias | Araujo M.S.,Laboratorio Of Enterobacterias | Reis E.M.F.,Laboratorio Of Enterobacterias | And 3 more authors.
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia | Year: 2016

We analyzed a total of 1824 strains of Salmonella isolated from swine-origin foods from January/2005 to June/2010. The strains from different regions of the country were received by Labent/IOC/FIOCRUZ for conclusive antigenic characterization. We identified 41 serovars, of which these stood out: Typhimurium, Derby, Enteritidis, Panama, Infantis and Anatum. Bacteriological and epidemiological aspects related to these serovars were discussed. The antimicrobial susceptibility test was performed on 357 samples, 257 (72%) were resistant to one or more of these drugs and 31,9% were multiresistant. A variety of serovars were identified reinforcing the swine as an important reservoir of Salmonella in the food chain. The high rates of antimicrobial resistance obtained in this evaluation may represent a risk condition to human health.

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