Planaltina, Brazil
Planaltina, Brazil

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Perez D.J.,Instituto Nacional de Tecnologia Agropecuaria | Perez D.J.,Laboratorio Of Ecotoxicologia | Perez D.J.,CONICET | Lukaszewicz G.,Laboratorio Of Ecotoxicologia | And 4 more authors.
Environmental Pollution | Year: 2011

The wetland macrophyte Bidens laevis possesses suitable cytological characteristics for genotoxicity testing. To test its sensitivity as compared to terrestrial plants species currently in use in standardized assays, Methyl Methanesulfonate (MMS), N-ethyl-N-nitrosourea (ENU) and Maleic Hydrazide (HM) were used. On the other hand, the insecticide Endosulfan (ES) - an environmentally relevant contaminant - was assayed in seeds and two-month old plants. Mitotic Index (MI), frequency of Chromosome Aberrations in Anaphase-Telophase (CAAT) and frequency of Abnormal Metaphases (AM) were analyzed. MH, MMS and ENU caused a significant decrease of the MI. MMS was aneugenic whereas MH and ENU were both aneugenic and clastogenic. ES caused a significant concentration-dependent increase of total- and aneugenic-CAAT in roots and a significant high frequency of AM at high concentrations. Because of its sensitivity to mutagenic substances, B. laevis can be regarded as a reliable and convenient species for genotoxicity assays especially if aquatic contaminants are evaluated. © 2010 Elsevier Ltd. All rights reserved.


Oliveira-Filho E.C.,Laboratorio Of Ecotoxicologia | De Freitas Muniz D.H.,Laboratorio Of Ecotoxicologia | Ferreira M.F.N.,University of Brasilia | Grisolia C.K.,University of Brasilia
Bulletin of Environmental Contamination and Toxicology | Year: 2010

The pyrometallurgical process of mining for obtaining ferronickel involves a stage of calcinations. At this stage a residue is generated described as a calcination dust of fine black grains. Analysis of this material revealed a significant presence of Fe, around 53,000 ppm and Ni, around 14,000, beyond of other metals as Al, Mn, and Cr. Adults and larvae of Oreochromis niloticus were used to evaluate acute toxicity, cytotoxicity and genotoxicity, and histopathological effects. The data obtained show absence of toxicity in concentrations of 5, 10 and 50% but a considerable potential for bioaccumulation in the fish's body. © 2010 Springer Science+Business Media, LLC.


Oliveira-Filho E.C.,Laboratorio Of Ecotoxicologia | Oliveira-Filho E.C.,Centro Universitario Of Brasilia | Filho J.S.,University of Brasilia | Novais L.A.,Centro Universitario Of Brasilia | And 3 more authors.
Environmental Science and Pollution Research | Year: 2016

This study aims to evaluate the effects of maghemite nanoparticles (γ-Fe2O3) coated with meso-2, 3-dimercaptosuccinic acid (DMSA) stabilizer on the survival and reproduction of the aquatic snail Biomphalaria glabrata. The cumulative means of egg masses and eggs per individual in the control group at the end of 4 weeks were 18.8 and 326.7, respectively. These values at the concentration of 1 mg/L were 17.2 and 291.6; at 10 mg/L, they were 19.6 and 334.4 ,and at 100 mg/L, they were 14.3 and 311.1. Results showed no significant differences between the tested and the control groups at the level of p < 0.05. Exposure of embryos for 10 days showed absence of mortality, malformation, or hatching delay. X-ray microtomography confirmed the presence of nanoparticles in exposed individuals and showed the complete elimination of the nanoparticles after 30 days in clean water. In the studied conditions, it is clear that γ-Fe2O3 coated with stabilizing DMSA did not alter the fecundity or the fertility of the snail B. glabrata after 4 weeks of exposure, and accumulation was not present after 30 days in clean water. © 2016 Springer-Verlag Berlin Heidelberg


Rivero-Wendt C.L.G.,University of Brasilia | Borges A.C.,University of Brasilia | Oliveira-Filho E.C.,Laboratorio Of Ecotoxicologia | Miranda-Vilela A.L.,University of Brasilia | And 2 more authors.
Genetics and Molecular Research | Year: 2014

17-α-methyltestosterone (MT) is a synthetic hormone used in fish hatcheries to induce male monosex. Snails hold promise as possible test models to assess chemicals acting on the endocrine system. Biomphalaria glabrata is an aquatic gastropod mollusk (Pulmonata, Planorbidae) that can be easily maintained in aquaria, predisposing the species for use in ecotoxicological testing. This study evaluated the reproductive effects of MT on B. glabrata by examining histological changes and its reproductive performance. Ten snails per group were exposed for 4 weeks to different concentrations of MT (0.01, 0.1, and 1.0 mg/L). The total number of laid eggs, egg mass per group, size of type V oocytes, and production of spermatozoids were determined. Reproduction of B. glabrata was affected by MT. At the lowest concentration (0.01 mg/L), MT caused a statistically significant increase in the number of egg mass per snail compared with controls unexposed to MT. Histopathology analyses showed an increase in the sperm production at the higher MT concentrations of 0.1 and 1.0 mg/L. Chromatographic analyses of water samples showed that MT concentrations rapidly declined within a 96-h period. These results highlight the importance of giving more support to regulatory authorities, since MT is not registered for use on fish hatcheries in many countries around the world. Wastewater from fish farms discharged into aquatic ecosystems should be monitored for MT residues, since its presence could compromise the reproduction of other native snail species. © FUNPEC-RP.


Muniz D.H.F.,Laboratorio Of Ecotoxicologia | Moraes A.S.,Centro Universitario Of Brasilia Uniceub | Freire I.S.,Centro Universitario Of Brasilia Uniceub | da Cruz C.J.D.,Laboratorio Of Ecotoxicologia | And 2 more authors.
Acta Limnologica Brasiliensia | Year: 2011

Introduction: Brazil's Federal District (FD) has seen steep population growth in recent years, and this has increased demand for high-quality water. Aim:The present work aims to evaluate the quality of surface water from two water bodies in the FD at six sampling points, three of which are in the Sobradinho River (the receiving body of a sewage treatment plant effluent) and three in the Jardim River (located in an agricultural region). Methods: Analyses were carried out every thirty days, for twelve months, covering rainy and dry seasons. Parameters were analyzed such as temperature, pH, dissolved oxygen (DO), conductivity, turbidity, total hardness, main ions, total and fecal coliforms (E. coli) in water. Ecotoxicological evaluation was also performed, using the micro-crustacean Ceriodaphnia dubia. Results: Data of physical and chemical determinations showed DO, conductivity, Cl -, NO 3 -, SO 4 2- and Na + to be important in indicating contamination by urban effluents. On the subject of biological parameters-E. coli and ecotoxicological evaluation-the former was seen to be more effective in the urban area. However, in the countryside, the assay with C. dubia proved to be the most sensitive, although less than ideal, because the organism is very sensitive to low water hardness. Conclusions: The fecal coliform indicator (E. coli) was the most effective one for comparing water quality in the two basins, mainly for the urbanized one, while ecotoxicity assays with C. dubia were harmed by the natural chemical composition of the water.


Oliveira-Filho E.C.,Laboratorio Of Ecotoxicologia | Oliveira-Filho E.C.,Centro Universitario Of Brasilia | Muniz D.H.F.,Laboratorio Of Ecotoxicologia | Freire I.S.,Laboratorio Of Ecotoxicologia | And 6 more authors.
Ecotoxicology | Year: 2011

Microbial pest control agents or entomopathogens have been considered an interesting alternative to use instead of chemical insecticides. Knowledge of ecotoxicity data is very important to predict the hazard of any product released in the environment and subsidize the regulation of these products by governmental agencies. In the present study four new Brazilian strains of Bacillus and one fungus were tested to evaluate their acute toxicity to the microcrustacean Daphnia similis, the snail Biomphalaria glabrata and the dung beetle Digitonthophagus gazella. The microcrustaceans and the snails were exposed to entomopathogens in synthetic softwater and the beetles were exposed directly in cattle dung. Obtained data reveal low susceptibility of the non-target species to tested microorganisms, with lethal concentrations being observed only at much higher concentrations than that effective against target insects. These results show that the tested strains are selective in their action mode and seem to be non-hazardous to non-target species. © 2011 Springer Science+Business Media, LLC.


Gutierrez M.F.,CONICET | Paggi J.C.,CONICET | Gagneten A.M.,Laboratorio Of Ecotoxicologia
Ecotoxicology | Year: 2012

The purpose of this work was to experimentally analyze the impact of copper, chromium and a commercial pesticide with endosulfan on the escape behavior of two copepods (Notodiaptomus conifer, Argyrodiaptomus falcifer) and three cladocerans (Daphnia magna, Pseudosida variabilis and Ceriodaphnia dubia). The experimental assays were carried out using a novel hydraulic devise designed to mimic three-speed predator capture behavior. Two concentrations, one ''high'' and one ''low'', were employed and the exposure time was 15 (±5) minutes. With two exceptions, the species exposed to heavy metals manifested higher ability to escape than controls. Both concentrations of the pesticide reduced the escape ability of cladocerans but copepods responded, in general, in a similar manner as for heavy metals. The immediate apparent advantage of low and early toxic effects is discussed and the high sensitivity of the escape behavior suggests that it could be a complementary endpoint to be used in future ecotoxicological tests. © 2011 Springer Science+Business Media, LLC.


Faria M.,University of Aveiro | Navas J.M.,Laboratorio Of Ecotoxicologia | Soares A.M.V.M.,University of Aveiro | Barata C.,CSIC - Institute of Environmental Assessment And Water Research
Science of the Total Environment | Year: 2014

There is limited data on the sub-lethal oxidative stress effects of titanium dioxide nanoparticle aggregates (NM-TiO2) and its modulation by simulated solar radiation (SSR) to aquatic organisms. This study aimed to examine sublethal oxidative stress effects of aqueous exposure to three different types of NM-TiO2 differing in their coating or crystal structure but of similar primary size (20nm) plus a micron-sized bulk material to zebrafish embryos without and with SSR. Oxidative stress responses of known model prooxidant (tert-Butyl hydroperoxide) and photoprooxidant (fluoranthene) compounds were also studied. Results evidenced a low bio-availability of NM-TiO2 to embryos with detrimental effects on growth at 1mgml-1. Phototoxicity increased moderately, by 3 and 1.5 fold, under co-exposures to fluoranthene (100μgl-1) and to the NM-TiO2 P25 (1mgml-1), respectively, being unchanged in the other TiO2 aggregates. In vitro exposures under SSR confirmed that the NM-TiO2 P25 had the highest potential to generate reactive oxygen species (ROS). Antioxidant enzyme activities of superoxide dismutase increased shortly after exposure to the studied materials, whereas the levels of glutathione tend to be altered after longer exposures. All compounds were able to produce oxidative stress enhancing the senescence-associated β galactosidase pigment (SA-β-gal). Under SSR radiation the NM-TiO2 P25 affected antioxidant and oxidative stress responses as the phototoxic compound fluoranthene. These results indicated that despite the low bio-availability of NM-TiO2 to zebrafish embryos, P25 was phototoxic due to the production of reactive oxygen species. Nevertheless, overall our results indicated that fish development may not be at high risk in the face of NM-TiO2, even when combined with prooxidant conditions. © 2013 Elsevier B.V.


PubMed | Centro Universitario Of Brasilia, Laboratorio Of Ecotoxicologia, Federal University of Rondônia and University of Brasilia
Type: Journal Article | Journal: Environmental science and pollution research international | Year: 2016

This study aims to evaluate the effects of maghemite nanoparticles (-Fe2O3) coated with meso-2, 3-dimercaptosuccinic acid (DMSA) stabilizer on the survival and reproduction of the aquatic snail Biomphalaria glabrata. The cumulative means of egg masses and eggs per individual in the control group at the end of 4weeks were 18.8 and 326.7, respectively. These values at the concentration of 1mg/L were 17.2 and 291.6; at 10mg/L, they were 19.6 and 334.4 ,and at 100mg/L, they were 14.3 and 311.1. Results showed no significant differences between the tested and the control groups at the level of p<0.05. Exposure of embryos for 10days showed absence of mortality, malformation, or hatching delay. X-ray microtomography confirmed the presence of nanoparticles in exposed individuals and showed the complete elimination of the nanoparticles after 30days in clean water. In the studied conditions, it is clear that -Fe2O3 coated with stabilizing DMSA did not alter the fecundity or the fertility of the snail B. glabrata after 4weeks of exposure, and accumulation was not present after 30days in clean water.


PubMed | State University of Goiás, Laboratorio Of Ecotoxicologia and University of Brasilia
Type: Journal Article | Journal: International journal of environmental research and public health | Year: 2015

The release of domestic sewage in water resources is a practical feature of the urbanization process, and this action causes changes that may impair the environmental balance and the water quality for several uses. The aim of this study was to evaluate the influence of urbanization on the surface water quality of the Preto River throughout the town of Formosa, Gois, Brazil. Samples were collected at five points along the river, spatially distributed from one side to the other of the town of Formosa, from May to October of 2012. Data were subjected to descriptive statistics, as well as variance and cluster analysis. Point P2, the first point after the city, showed the worst water quality indicators, mainly with respect to the total and fecal coliform parameters, as well as nitrate concentrations. These results may be related to the fact that this point is located on the outskirts of the town, an area under urbanization and with problems of sanitation, including absence of sewage collection and treatment. The data observed in this monitoring present a public health concern because the water body is used for bathing, mainly in parts of Feia Lagoon. The excess of nutrients is a strong indicator of water eutrophication and should alert decision-makers to the need for preservation policies.

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