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Loiola S.,State University of Rio de Janeiro | Carvalho R.S.,State University of Rio de Janeiro | Bergallo H.G.,State University of Rio de Janeiro | Weksler M.,Laboratorio Of Ecoepidemiologia Da Doenca Of Chagas | And 2 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2015

The identification of species and the assignment of unidentified samples to a voucher reference database are among the most requested forensic analyses involving crimes against wildlife. Genomic DNA from forensic or museum samples, however, are frequently degraded, hampering the analysis of DNA fragments longer than 500. bp. Among studies targeting non-human species identification, one of the most frequently used fragments is the mitochondrial Cytochrome C Oxidase subunit I (COI), in particular a 'barcode' region containing approximately 650 base pairs (bp). The main objective of this work was to develop a set of primers to amplify five shorter overlapping COI fragments for the neotropical marmosets (Callithrix spp.) to be used in samples from museum specimens. Taxidermized skins, bones, and hair tissues were sampled out of 9 museum specimens with a range archival from 81 to 2 years; PCR were performed with five mini-amplicons primers pairs and with the primer pair for the whole barcode COI fragment, followed by Sanger sequencing. As results, no amplification was observed using the primer pair for the longer COI sequence; however, using the new set of mini-amplicon primers, all types of samples were amplified. We established optimal PCR conditions for the employed primers and conclude that the mini-amplicon strategy for COI region typing is best suited for hardly degraded samples in forensic research, and in particular for museum samples. © 2015 Elsevier Ireland Ltd. Source


Delciellos A.C.,Federal University of Rio de Janeiro | Aguieiras M.,State University of Rio de Janeiro | Geise L.,State University of Rio de Janeiro | Weksler M.,Laboratorio Of Ecoepidemiologia Da Doenca Of Chagas | Rocha-Barbosa O.,State University of Rio de Janeiro
Check List | Year: 2015

Here we report the first record of Drymoreo­ mys albimaculatus Percequillo, Weksler & Costa, 2011 (Cricetidae, Sigmodontinae) in Rio de Janeiro state, Brazil. One specimen was captured at Serra da Bocaina National Park, municipality of Paraty. The specimen was captured in a pitfall trap, in a mixed habitat of forest and bamboo. The karyotype showed 2n = 62 and FN = 62, which is similar to the previously described for the species. © 2015 Check List and Authors. Source


da Mota F.F.,Laboratorio Of Biologia Computacional E Sistemas | Marinho L.P.,Laboratorio Of Bioquimica E Fisiologia Of Insetos | de Moreira C.J.C.,Laboratorio Of Doencas Parasitarias | Lima M.M.,Laboratorio Of Ecoepidemiologia Da Doenca Of Chagas | And 4 more authors.
PLoS Neglected Tropical Diseases | Year: 2012

Background: Chagas disease is a trypanosomiasis whose agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs known as triatomines. Even though insecticide treatments allow effective control of these bugs in most Latin American countries where Chagas disease is endemic, the disease still affects a large proportion of the population of South America. The features of the disease in humans have been extensively studied, and the genome of the parasite has been sequenced, but no effective drug is yet available to treat Chagas disease. The digestive tract of the insect vectors in which T. cruzi develops has been much less well investigated than blood from its human hosts and constitutes a dynamic environment with very different conditions. Thus, we investigated the composition of the predominant bacterial species of the microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and Dipetalogaster genera. Methodology/Principal Findings: Microbiota of triatomine guts were investigated using cultivation-independent methods, i.e., phylogenetic analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE) and cloned-based sequencing. The Chao index showed that the diversity of bacterial species in triatomine guts is low, comprising fewer than 20 predominant species, and that these species vary between insect species. The analyses showed that Serratia predominates in Rhodnius, Arsenophonus predominates in Triatoma and Panstrongylus, while Candidatus Rohrkolberia predominates in Dipetalogaster. Conclusions/Significance: The microbiota of triatomine guts represents one of the factors that may interfere with T. cruzi transmission and virulence in humans. The knowledge of its composition according to insect species is important for designing measures of biological control for T. cruzi. We found that the predominant species of the bacterial microbiota in triatomines form a group of low complexity whose structure differs according to the vector genus. © 2012 da Mota et al. Source


Gomes T.F.,Laboratorio Of Ecoepidemiologia Da Doenca Of Chagas
World health & population | Year: 2013

Interaction between Chagas disease vectors and man is continuous in vulnerable dwellings, in which the vectors feed on man and find conditions for reproduction. This study explores factors that affect the choice of home construction methods in a rural community in Brazil, emphasizing the rationale for the persistence of dwelling vulnerability. Information on local resident perspectives regarding safety and home construction methods was gathered through domiciliary interviews with open questionnaires. The study revealed a large proportion of vulnerable mud huts, with others under construction. Insecurity over land tenure inhibits the construction of definitive houses. Mud homes are associated with greater structural stability. Cultural and economic factors have clearly been linked to the choice of method for home construction. The economic evolution of family conflicts with traditional aspects as well as the relative increased cost of the materials needed for mud house construction has not completely inhibited building with mud. Copyright © 2013 Longwoods Publishing. Source


Valenca-Barbosa C.,Laboratorio Of Ecoepidemiologia Da Doenca Of Chagas | Valenca-Barbosa C.,Laboratorio Of Estudos Integrados Em Protozoologia | Valenca-Barbosa C.,University Paris - Sud | Valenca-Barbosa C.,Federal University of Paraiba | And 31 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2015

We used the gut contents of triatomines collected from rural areas of Ceará State, northeastern Brazil, to identify their putative hosts via vertebrate cytb gene sequencing. Successful direct sequencing was obtained for 48% of insects, comprising 50 Triatoma brasiliensis, 7 Triatoma pseudomaculata, and 1 Rhodnius nasutus. Basic local alignment search tool (BLAST) procedure revealed that domestic animals, such as chickens (Gallus gallus) and goats (Capra hircus), are the main food source, including in sylvatic environment. Native hosts were also detected in peridomestic environment such as reptiles (Tropidurus sp. and Iguana iguana) and the Galea spixii (Rodentia: Caviidae). The role of goats and Galea spixii in Chagas disease epidemiology calls for further studies, because these mammals likely link the sylvatic and domestic Trypanosoma cruzi cycles. Source

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